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Expression of Xenobiotic-Metabolizing Cytochrome P450s in Human Pulmonary Tissues

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Diversification in Toxicology — Man and Environment

Part of the book series: Archives of Toxicology ((TOXICOLOGY,volume 20))

Abstract

The purpose of the study was to obtain a comprehensive picture of the expression of cytochrome P450s (CYP) in the human lung, broncho-alveolar macrophages (BAM), and peripheral blood lymphocytes. The methods used were reverse transcriptase-polymerase chain reaction (RT-PCR) with gene-specific primers and immunohistochemistry with specific anti-peptide antibodies. In RT-PCR, CYPs 1A1, 2B6/7, 2E1, 2F1, 3A5 and 4B1 were detected in cDNA prepared from whole lung tissue. BAMs expressed CYPs 1B1, 2B6/7, 2C, 2E1, 2F1, 3A5 and 4B1. These tissues lacked CYPs IA2, 2A6, 2D6 and 3A7. In peripheral blood lymphocytes, only CYP1B1 and CYP2E1 mRNAs were consistently detected. In immunohistochemistry with anti-CYP3A antibodies, epithelial staining of CYP3A5 was observed in 100% of individuals, while only about 20% exhibited CYP3A4 staining. CYP3A5 protein was localized in the bronchial wall, bronchial glands, bronchiolar epithelium, alveolar epithelium, vascular endothelium and alveolar macrophages. The results indicate that several different xenobiotic-metabolizing CYPs are present in the human lung, possibly contributing to in situ activation of pulmonary procarcinogens.

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References

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© 1998 Springer-Verlag Berlin Heidelberg

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Raunio, H. et al. (1998). Expression of Xenobiotic-Metabolizing Cytochrome P450s in Human Pulmonary Tissues. In: Seiler, J.P., Autrup, J.L., Autrup, H. (eds) Diversification in Toxicology — Man and Environment. Archives of Toxicology, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-46856-8_41

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  • DOI: https://doi.org/10.1007/978-3-642-46856-8_41

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-46858-2

  • Online ISBN: 978-3-642-46856-8

  • eBook Packages: Springer Book Archive

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