Abstract
Lymphatic vessels are essential for the maintenance of normal tissue fluid balance and immune surveillance, but they also provide a pathway for metastasis in many types of cancers (reviewed in Alitalo and Carmeliet 2002). In spite of the importance of lymphatic vessels in medicine, the cell biology of this part of the vascular system has received little attention until recently. Only few lymphatic endothelial cell lines have been available for molecular biological studies, and these were mainly derived from lymphatic tumors. However, the identification of lymphatic specific markers during the past few years and the isolation and maintenance of primary cultures of lymphatic endothelial cells have enabled studies of the molecular properties of these cells.
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© 2004 Springer-Verlag Berlin Heidelberg
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Mäkinen, T., Alitalo, K. (2004). Detection, Isolation and Culture of Lymphatic Endothelial Cells. In: Augustin, H.G. (eds) Methods in Endothelial Cell Biology. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-18725-4_5
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DOI: https://doi.org/10.1007/978-3-642-18725-4_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-21397-0
Online ISBN: 978-3-642-18725-4
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