Abstract
Cells adhere to the extracellular matrix through interaction with adhesive extracellular matrix glycoproteins, including fibronectin, laminins, vitronectin, thrombospondins, tenascins, entactins (or nidogens), nephronectin, fibrinogen, and others. Most adhesive glycoproteins bind cells through cell surface integrin receptors in conjunction with other cell surface receptors, such as dystroglycans and syndecans, and interact with other extracellular matrix proteins to form an intensive matrix network. Interactions between cells and the extracellular matrix may mediate many cellular responses, such as cell migration, growth, differentiation, and survival. Cells receive and respond to signals from surrounding extracellular matrix, and in turn, modulate surrounding extracellular matrix through control of matrix assembly. This chapter discusses the adhesive glycoproteins and focuses on the interaction between integrins and adhesive glycoproteins.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adams JC (1997) Thrombospondin-1. Int J Biochem Cell Biol 29:861–865
Adams JC, Monk R, Taylor AL, Ozbek S, Fascetti N, Baumgartner S, Engel J (2003) Characterisation of Drosophila thrombospondin defines an early origin of pentameric thrombospondins. J Mol Biol 328:479–494
Akiyama SK, Yamada SS, Chen WT, Yamada KM (1989) Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization. J Cell Biol 109:863–875
Ambort D, Brellier F, Becker-Pauly C, Stocker W, Andrejevic-Blant S, Chiquet M, Sterchi EE (2010) Specific processing of tenascin-C by the metalloprotease meprinbeta neutralizes its inhibition of cell spreading. Matrix Biol 29:31–42
Andrews MR, Czvitkovich S, Dassie E, Vogelaar CF, Faissner A, Blits B, Gage FH, Ffrench-Constant C, Fawcett JW (2009) Alpha9 integrin promotes neurite outgrowth on tenascin-C and enhances sensory axon regeneration. J Neurosci 29:5546–5557
Aota S, Nomizu M, Yamada KM (1994) The short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function. J Biol Chem 269:24756–24761
Bae E, Sakai T, Mosher DF (2004) Assembly of exogenous fibronectin by fibronectin-null cells is dependent on the adhesive substrate. J Biol Chem 279:35749–35759
Bagavandoss P, Wilks JW (1990) Specific inhibition of endothelial cell proliferation by thrombospondin. Biochem Biophys Res Commun 170:867–872
Banno A, Ginsberg MH (2008) Integrin activation. Biochem Soc Trans 36:229–234
Barkalow FJ, Schwarzbauer JE (1991) Localization of the major heparin-binding site in fibronectin. J Biol Chem 266:7812–7818
Baron M, Norman D, Willis A, Campbell ID (1990) Structure of the fibronectin type 1 module. Nature 345:642–646
Bazigou E, Xie S, Chen C, Weston A, Miura N, Sorokin L, Adams R, Muro AF, Sheppard D, Makinen T (2009) Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis. Dev Cell 17:175–186
Belkin AM, Stepp MA (2000) Integrins as receptors for laminins. Microsc Res Tech 51:280–301
Bernard BA, Yamada KM, Olden K (1982) Carbohydrates selectively protect a specific domain of fibronectin against proteases. J Biol Chem 257:8549–8554
Beyth RJ, Culp LA (1984) Complementary adhesive responses of human skin fibroblasts to the cell-binding domain of fibronectin and the heparan sulfate-binding protein, platelet factor-4. Exp Cell Res 155:537–548
Bingham RJ, Rudino-Pinera E, Meenan NA, Schwarz-Linek U, Turkenburg JP, Hook M, Garman EF, Potts JR (2008) Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains. Proc Natl Acad Sci USA 105:12254–12258
Bork P, Doolittle RF (1992) Proposed acquisition of an animal protein domain by bacteria. Proc Natl Acad Sci USA 89:8990–8994
Brandenberger R, Schmidt A, Linton J, Wang D, Backus C, Denda S, Muller U, Reichardt LF (2001) Identification and characterization of a novel extracellular matrix protein nephronectin that is associated with integrin alpha8beta1 in the embryonic kidney. J Cell Biol 154:447–458
Brooks PC, Clark RA, Cheresh DA (1994) Requirement of vascular integrin alpha v beta 3 for angiogenesis. Science 264:569–571
Burton-Wurster N, Gendelman R, Chen H, Gu DN, Tetreault JW, Lust G, Schwarzbauer JE, MacLeod JN (1999) The cartilage-specific (V + C)- fibronectin isoform exists primarily in homodimeric and monomeric configurations. Biochem J 341(Pt 3):555–561
Cardin AD, Weintraub HJ (1989) Molecular modeling of protein-glycosaminoglycan interactions. Arteriosclerosis 9:21–32
Carlson CB, Bernstein DA, Annis DS, Misenheimer TM, Hannah BL, Mosher DF, Keck JL (2005) Structure of the calcium-rich signature domain of human thrombospondin-2. Nat Struct Mol Biol 12:910–914
Carnemolla B, Leprini A, Allemanni G, Saginati M, Zardi L (1992) The inclusion of the type III repeat ED-B in the fibronectin molecule generates conformational modifications that unmask a cryptic sequence. J Biol Chem 267:24689–24692
Chen H, Sottile J, O’Rourke KM, Dixit VM, Mosher DF (1994) Properties of recombinant mouse thrombospondin 2 expressed in Spodoptera cells. J Biol Chem 269:32226–32232
Cho J, Mosher DF (2006) Enhancement of thrombogenesis by plasma fibronectin cross-linked to fibrin and assembled in platelet thrombi. Blood 107:3555–3563
Collier IE, Wilhelm SM, Eisen AZ, Marmer BL, Grant GA, Seltzer JL, Kronberger A, He CS, Bauer EA, Goldberg GI (1988) H-ras oncogene-transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen. J Biol Chem 263:6579–6587
Colognato H, Yurchenco PD (2000) Form and function: the laminin family of heterotrimers. Dev Dyn 218:213–234
Colucci S, Giannelli G, Grano M, Faccio R, Quaranta V, Zallone AZ (1996) Human osteoclast-like cells selectively recognize laminin isoforms, an event that induces migration and activates Ca2+ mediated signals. J Cell Sci 109(Pt 6):1527–1535
Constantine KL, Brew SA, Ingham KC, Llinas M (1992) 1H-n.m.r. studies of the fibronectin 13 kDa collagen-binding fragment. Evidence for autonomous conserved type I and type II domain folds. Biochem J 283(Pt 1):247–254
Coussen F, Choquet D, Sheetz MP, Erickson HP (2002) Trimers of the fibronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation. J Cell Sci 115:2581–2590
Curnis F, Longhi R, Crippa L, Cattaneo A, Dondossola E, Bachi A, Corti A (2006) Spontaneous formation of L-isoaspartate and gain of function in fibronectin. J Biol Chem 281:36466–36476
Darribere T, Guida K, Larjava H, Johnson KE, Yamada KM, Thiery JP, Boucaut JC (1990) In vivo analyses of integrin beta 1 subunit function in fibronectin matrix assembly. J Cell Biol 110:1813–1823
Dickinson CD, Gay DA, Parello J, Ruoslahti E, Ely KR (1994a) Crystals of the cell-binding module of fibronectin obtained from a series of recombinant fragments differing in length. J Mol Biol 238:123–127
Dickinson CD, Veerapandian B, Dai XP, Hamlin RC, Xuong NH, Ruoslahti E, Ely KR (1994b) Crystal structure of the tenth type III cell adhesion module of human fibronectin. J Mol Biol 236:1079–1092
Dong LJ, Hsieh JC, Chung AE (1995) Two distinct cell attachment sites in entactin are revealed by amino acid substitutions and deletion of the RGD sequence in the cysteine-rich epidermal growth factor repeat 2. J Biol Chem 270:15838–15843
Dzamba BJ, Jakab KR, Marsden M, Schwartz MA, DeSimone DW (2009) Cadherin adhesion, tissue tension, and noncanonical Wnt signaling regulate fibronectin matrix organization. Dev Cell 16:421–432
Elices MJ, Tsai V, Strahl D, Goel AS, Tollefson V, Arrhenius T, Wayner EA, Gaeta FC, Fikes JD, Firestein GS (1994) Expression and functional significance of alternatively spliced CS1 fibronectin in rheumatoid arthritis microvasculature. J Clin Invest 93:405–416
Engel J, Odermatt E, Engel A, Madri JA, Furthmayr H, Rohde H, Timpl R (1981) Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix. J Mol Biol 150:97–120
Engvall E, Ruoslahti E, Miller EJ (1978) Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med 147:1584–1595
Ensenberger MG, Annis DS, Mosher DF (2004) Actions of the functional upstream domain of protein F1 of Streptococcus pyogenes on the conformation of fibronectin. Biophys Chem 112:201–207
Erat MC, Slatter DA, Lowe ED, Millard CJ, Farndale RW, Campbell ID, Vakonakis I (2009) Identification and structural analysis of type I collagen sites in complex with fibronectin fragments. Proc Natl Acad Sci USA 106:4195–4200
Erickson HP, Carrell N, McDonagh J (1981) Fibronectin molecule visualized in electron microscopy: a long, thin, flexible strand. J Cell Biol 91:673–678
Esemuede N, Lee T, Pierre-Paul D, Sumpio BE, Gahtan V (2004) The role of thrombospondin-1 in human disease. J Surg Res 122:135–142
Etheredge RE, Han S, Fossel E, Tanzer ML, Glimcher MJ (1985) Identification and quantitation of O-phosphoserine in human plasma fibronectin. FEBS Lett 186:259–262
Felding-Habermann B, Cheresh DA (1993) Vitronectin and its receptors. Curr Opin Cell Biol 5:864–868
ffrench-Constant C (1995) Alternative splicing of fibronectin – many different proteins but few different functions. Exp Cell Res 221:261–271
Fogerty FJ, Akiyama SK, Yamada KM, Mosher DF (1990) Inhibition of binding of fibronectin to matrix assembly sites by anti-integrin (alpha 5 beta 1) antibodies. J Cell Biol 111:699–708
Fox JW, Mayer U, Nischt R, Aumailley M, Reinhardt D, Wiedemann H, Mann K, Timpl R, Krieg T, Engel J et al (1991) Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV. EMBO J 10:3137–3146
Frazier WA (1991) Thrombospondins. Curr Opin Cell Biol 3:792–799
Friedland JC, Lee MH, Boettiger D (2009) Mechanically activated integrin switch controls alpha5beta1 function. Science 323:642–644
Fukuda T, Yoshida N, Kataoka Y, Manabe R, Mizuno-Horikawa Y, Sato M, Kuriyama K, Yasui N, Sekiguchi K (2002) Mice lacking the EDB segment of fibronectin develop normally but exhibit reduced cell growth and fibronectin matrix assembly in vitro. Cancer Res 62:5603–5610
Gechtman Z, Belleli A, Lechpammer S, Shaltiel S (1997) The cluster of basic amino acids in vitronectin contributes to its binding of plasminogen activator inhibitor-1: evidence from thrombin-, elastase- and plasmin-cleaved vitronectins and anti-peptide antibodies. Biochem J 325(Pt 2):339–349
Geiger B, Bershadsky A, Pankov R, Yamada KM (2001) Transmembrane crosstalk between the extracellular matrix–cytoskeleton crosstalk. Nat Rev Mol Cell Biol 2:793–805
George EL, Georges-Labouesse EN, Patel-King RS, Rayburn H, Hynes RO (1993) Defects in mesoderm, neural tube and vascular development in mouse embryos lacking fibronectin. Development 119:1079–1091
Giancotti FG, Ruoslahti E (1990) Elevated levels of the alpha 5 beta 1 fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Cell 60:849–859
Ginsberg MH, Partridge A, Shattil SJ (2005) Integrin regulation. Curr Opin Cell Biol 17:509–516
Givant-Horwitz V, Davidson B, Reich R (2005) Laminin-induced signaling in tumor cells. Cancer Lett 223:1–10
Gonzales M, Haan K, Baker SE, Fitchmun M, Todorov I, Weitzman S, Jones JC (1999) A cell signal pathway involving laminin-5, alpha3beta1 integrin, and mitogen-activated protein kinase can regulate epithelial cell proliferation. Mol Biol Cell 10:259–270
Gresham HD, Graham IL, Griffin GL, Hsieh JC, Dong LJ, Chung AE, Senior RM (1996) Domain-specific interactions between entactin and neutrophil integrins. G2 domain ligation of integrin alpha3beta1 and E domain ligation of the leukocyte response integrin signal for different responses. J Biol Chem 271:30587–30594
Guan JL, Hynes RO (1990) Lymphoid cells recognize an alternatively spliced segment of fibronectin via the integrin receptor alpha 4 beta 1. Cell 60:53–61
Halliday NL, Tomasek JJ (1995) Mechanical properties of the extracellular matrix influence fibronectin fibril assembly in vitro. Exp Cell Res 217:109–117
Harburger DS, Calderwood DA (2009) Integrin signalling at a glance. J Cell Sci 122:159–163
Hintermann E, Quaranta V (2004) Epithelial cell motility on laminin-5: regulation by matrix assembly, proteolysis, integrins and erbB receptors. Matrix Biol 23:75–85
Hirano H, Yamada Y, Sullivan M, de Crombrugghe B, Pastan I, Yamada KM (1983) Isolation of genomic DNA clones spanning the entire fibronectin gene. Proc Natl Acad Sci USA 80:46–50
Hsieh JC, Wu C, Chung AE (1994) The binding of fibronectin to entactin is mediated through the 29 kDa amino terminal fragment of fibronectin and the G2 domain of entactin. Biochem Biophys Res Commun 199:1509–1517
Huang JT, Lee V (2005) Identification and characterization of a novel human nephronectin gene in silico. Int J Mol Med 15:719–724
Huang W, Chiquet-Ehrismann R, Moyano JV, Garcia-Pardo A, Orend G (2001) Interference of tenascin-C with syndecan-4 binding to fibronectin blocks cell adhesion and stimulates tumor cell proliferation. Cancer Res 61:8586–8594
Humphries JD, Byron A, Humphries MJ (2006) Integrin ligands at a glance. J Cell Sci 119: 3901–3903
Huveneers S, Truong H, Fassler R, Sonnenberg A, Danen EH (2008) Binding of soluble fibronectin to integrin alpha5 beta1 - link to focal adhesion redistribution and contractile shape. J Cell Sci 121:2452–2462
Hynes R (1990) Fibronectins. Springer, New York
Hynes RO (2002) Integrins: bidirectional, allosteric signaling machines. Cell 110:673–687
Hynes RO (2009) The extracellular matrix: not just pretty fibrils. Science 326:1216–1219
Ido H, Harada K, Futaki S, Hayashi Y, Nishiuchi R, Natsuka Y, Li S, Wada Y, Combs AC, Ervasti JM, Sekiguchi K (2004) Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10. J Biol Chem 279:10946–10954
Ido H, Harada K, Yagi Y, Sekiguchi K (2006) Probing the integrin-binding site within the globular domain of laminin-511 with the function-blocking monoclonal antibody 4C7. Matrix Biol 25:112–117
Ido H, Ito S, Taniguchi Y, Hayashi M, Sato-Nishiuchi R, Sanzen N, Hayashi Y, Futaki S, Sekiguchi K (2008) Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains. J Biol Chem 283:28149–28157
Ido H, Nakamura A, Kobayashi R, Ito S, Li S, Futaki S, Sekiguchi K (2007) The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J Biol Chem 282:11144–11154
Ingham KC, Brew SA, Atha DH (1990) Interaction of heparin with fibronectin and isolated fibronectin domains. Biochem J 272:605–611
Ingham KC, Brew SA, Novokhatny VV (1995) Influence of carbohydrate on structure, stability, and function of gelatin-binding fragments of fibronectin. Arch Biochem Biophys 316:235–240
Izzard CS, Radinsky R, Culp LA (1986) Substratum contacts and cytoskeletal reorganization of BALB/c 3T3 cells on a cell-binding fragment and heparin-binding fragments of plasma fibronectin. Exp Cell Res 165:320–336
Jarnagin WR, Rockey DC, Koteliansky VE, Wang SS, Bissell DM (1994) Expression of variant fibronectins in wound healing: cellular source and biological activity of the EIIIA segment in rat hepatic fibrogenesis. J Cell Biol 127:2037–2048
Jones FS, Jones PL (2000) The tenascin family of ECM glycoproteins: structure, function, and regulation during embryonic development and tissue remodeling. Dev Dyn 218:235–259
Jones GE, Arumugham RG, Tanzer ML (1986) Fibronectin glycosylation modulates fibroblast adhesion and spreading. J Cell Biol 103:1663–1670
Joshi P, Chung CY, Aukhil I, Erickson HP (1993) Endothelial cells adhere to the RGD domain and the fibrinogen-like terminal knob of tenascin. J Cell Sci 106(Pt 1):389–400
Kibbey MC, Grant DS, Kleinman HK (1992) Role of the SIKVAV site of laminin in promotion of angiogenesis and tumor growth: an in vivo Matrigel model. J Natl Cancer Inst 84:1633–1638
Kohfeldt E, Sasaki T, Gohring W, Timpl R (1998) Nidogen-2: a new basement membrane protein with diverse binding properties. J Mol Biol 282:99–109
Kornblihtt AR, Pesce CG, Alonso CR, Cramer P, Srebrow A, Werbajh S, Muro AF (1996) The fibronectin gene as a model for splicing and transcription studies. FASEB J 10:248–257
Kosmehl H, Berndt A, Katenkamp D (1996) Molecular variants of fibronectin and laminin: structure, physiological occurrence and histopathological aspects. Virchows Arch 429: 311–322
Kost C, Stuber W, Ehrlich HJ, Pannekoek H, Preissner KT (1992) Mapping of binding sites for heparin, plasminogen activator inhibitor-1, and plasminogen to vitronectin's heparin-binding region reveals a novel vitronectin-dependent feedback mechanism for the control of plasmin formation. J Biol Chem 267:12098–12105
Kubota S, Tashiro K, Yamada Y (1992) Signaling site of laminin with mitogenic activity. J Biol Chem 267:4285–4288
Kvansakul M, Adams JC, Hohenester E (2004) Structure of a thrombospondin C-terminal fragment reveals a novel calcium core in the type 3 repeats. EMBO J 23:1223–1233
Kvansakul M, Hopf M, Ries A, Timpl R, Hohenester E (2001) Structural basis for the high-affinity interaction of nidogen-1 with immunoglobulin-like domain 3 of perlecan. EMBO J 20:5342–5346
Lark MW, Laterra J, Culp LA (1985) Close and focal contact adhesions of fibroblasts to a fibronectin-containing matrix. Fed Proc 44:394–403
Laterra J, Norton EK, Izzard CS, Culp LA (1983a) Contact formation by fibroblasts adhering to heparan sulfate-binding substrata (fibronectin or platelet factor 4). Exp Cell Res 146:15–27
Laterra J, Silbert JE, Culp LA (1983b) Cell surface heparan sulfate mediates some adhesive responses to glycosaminoglycan-binding matrices, including fibronectin. J Cell Biol 96: 112–123
Lawler J (2000) The functions of thrombospondin-1 and-2. Curr Opin Cell Biol 12:634–640
Lawler J, Hynes RO (1989) An integrin receptor on normal and thrombasthenic platelets that binds thrombospondin. Blood 74:2022–2027
Lawler J, Weinstein R, Hynes RO (1988) Cell attachment to thrombospondin: the role of ARG-GLY-ASP, calcium, and integrin receptors. J Cell Biol 107:2351–2361
Leahy DJ, Aukhil I, Erickson HP (1996) 2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region. Cell 84:155–164
Leikina E, Mertts MV, Kuznetsova N, Leikin S (2002) Type I collagen is thermally unstable at body temperature. Proc Natl Acad Sci USA 99:1314–1318
Leiss M, Beckmann K, Giros A, Costell M, Fassler R (2008) The role of integrin binding sites in fibronectin matrix assembly in vivo. Curr Opin Cell Biol 20:502–507
Li S, Harrison D, Carbonetto S, Fassler R, Smyth N, Edgar D, Yurchenco PD (2002) Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation. J Cell Biol 157:1279–1290
Liao YF, Gotwals PJ, Koteliansky VE, Sheppard D, Van De Water L (2002) The EIIIA segment of fibronectin is a ligand for integrins alpha 9beta 1 and alpha 4beta 1 providing a novel mechanism for regulating cell adhesion by alternative splicing. J Biol Chem 277:14467–14474
Linton JM, Martin GR, Reichardt LF (2007) The ECM protein nephronectin promotes kidney development via integrin alpha8beta1-mediated stimulation of Gdnf expression. Development 134:2501–2509
Liu MC, Lipmann F (1985) Isolation of tyrosine-O-sulfate by Pronase hydrolysis from fibronectin secreted by Fujinami sarcoma virus-infected rat fibroblasts. Proc Natl Acad Sci USA 82:34–37
Lotz MM, Burdsal CA, Erickson HP, McClay DR (1989) Cell adhesion to fibronectin and tenascin: quantitative measurements of initial binding and subsequent strengthening response. J Cell Biol 109:1795–1805
MacLeod JN, Burton-Wurster N, Gu DN, Lust G (1996) Fibronectin mRNA splice variant in articular cartilage lacks bases encoding the V, III-15, and I-10 protein segments. J Biol Chem 271:18954–18960
Magnusson MK, Mosher DF (1998) Fibronectin: structure, assembly, and cardiovascular implications. Arterioscler Thromb Vasc Biol 18:1363–1370
Main AL, Harvey TS, Baron M, Boyd J, Campbell ID (1992) The three-dimensional structure of the tenth type III module of fibronectin: an insight into RGD-mediated interactions. Cell 71:671–678
Majack RA, Goodman LV, Dixit VM (1988) Cell surface thrombospondin is functionally essential for vascular smooth muscle cell proliferation. J Cell Biol 106:415–422
Malinda KM, Kleinman HK (1996) The laminins. Int J Biochem Cell Biol 28:957–959
Manabe R, Ohe N, Maeda T, Fukuda T, Sekiguchi K (1997) Modulation of cell-adhesive activity of fibronectin by the alternatively spliced EDA segment. J Cell Biol 139:295–307
Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K (2008) Transcriptome-based systematic identification of extracellular matrix proteins. Proc Natl Acad Sci USA 105:12849–12854
Mao Y, Schwarzbauer JE (2005) Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biol 24:389–399
Marshall JF, Rutherford DC, McCartney AC, Mitjans F, Goodman SL, Hart IR (1995) Alpha v beta 1 is a receptor for vitronectin and fibrinogen, and acts with alpha 5 beta 1 to mediate spreading on fibronectin. J Cell Sci 108(Pt 3):1227–1238
Mayer U, Kohfeldt E, Timpl R (1998) Structural and genetic analysis of laminin-nidogen interaction. Ann NY Acad Sci 857:130–142
McDonald JA, Quade BJ, Broekelmann TJ, LaChance R, Forsman K, Hasegawa E, Akiyama S (1987) Fibronectin’s cell-adhesive domain and an amino-terminal matrix assembly domain participate in its assembly into fibroblast pericellular matrix. J Biol Chem 262:2957–2967
McKeown-Longo PJ, Mosher DF (1985) Interaction of the 70,000-mol-wt amino-terminal fragment of fibronectin with the matrix-assembly receptor of fibroblasts. J Cell Biol 100:364–374
Meloty-Kapella CV, Degen M, Chiquet-Ehrismann R, Tucker RP (2008) Effects of tenascin-W on osteoblasts in vitro. Cell Tissue Res 334:445–455
Menrad A, Menssen HD (2005) ED-B fibronectin as a target for antibody-based cancer treatments. Expert Opin Ther Targets 9:491–500
Mercado ML, Nur-e-Kamal A, Liu HY, Gross SR, Movahed R, Meiners S (2004) Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated by neuronal alpha7beta1 integrin. J Neurosci 24:238–247
Meredith JE Jr, Winitz S, Lewis JM, Hess S, Ren XD, Renshaw MW, Schwartz MA (1996) The regulation of growth and intracellular signaling by integrins. Endocr Rev 17:207–220
Midwood KS, Schwarzbauer JE (2002) Tenascin-C modulates matrix contraction via focal adhesion kinase- and Rho-mediated signaling pathways. Mol Biol Cell 13:3601–3613
Millard CJ, Campbell ID, Pickford AR (2005) Gelatin binding to the 8F19F1 module pair of human fibronectin requires site-specific N-glycosylation. FEBS Lett 579:4529–4534
Miner JH, Yurchenco PD (2004) Laminin functions in tissue morphogenesis. Annu Rev Cell Dev Biol 20:255–284
Morrison PR, Edsall JT, Miller SG (1948) Preparation and properties of serum and plasma proteins; the separation of purified fibrinogen from fraction I of human plasma. J Am Chem Soc 70:3103–3108
Mosesson MW, Umfleet RA (1970) The cold-insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. J Biol Chem 245:5728–5736
Mosher D (1989) Fibronectin. Academic, San Diego
Mosher DF (1990) Physiology of thrombospondin. Annu Rev Med 41:85–97
Mostafavi-Pour Z, Askari JA, Whittard JD, Humphries MJ (2001) Identification of a novel heparin-binding site in the alternatively spliced IIICS region of fibronectin: roles of integrins and proteoglycans in cell adhesion to fibronectin splice variants. Matrix Biol 20:63–73
Mould AP, Askari JA, Aota S, Yamada KM, Irie A, Takada Y, Mardon HJ, Humphries MJ (1997) Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies. Evidence that the synergy sequence of fibronectin is recognized by the amino-terminal repeats of the alpha5 subunit. J Biol Chem 272: 17283–17292
Mould AP, Komoriya A, Yamada KM, Humphries MJ (1991) The CS5 peptide is a second site in the IIICS region of fibronectin recognized by the integrin alpha 4 beta 1. Inhibition of alpha 4 beta 1 function by RGD peptide homologues. J Biol Chem 266:3579–3585
Moyano JV, Carnemolla B, Albar JP, Leprini A, Gaggero B, Zardi L, Garcia-Pardo A (1999) Cooperative role for activated alpha4 beta1 integrin and chondroitin sulfate proteoglycans in cell adhesion to the heparin III domain of fibronectin. Identification of a novel heparin and cell binding sequence in repeat III5. J Biol Chem 274:135–142
Moyano JV, Carnemolla B, Dominguez-Jimenez C, Garcia-Gila M, Albar JP, Sanchez-Aparicio P, Leprini A, Querze G, Zardi L, Garcia-Pardo A (1997) Fibronectin type III5 repeat contains a novel cell adhesion sequence, KLDAPT, which binds activated alpha4beta1 and alpha4beta7 integrins. J Biol Chem 272:24832–24836
Muller U, Wang D, Denda S, Meneses JJ, Pedersen RA, Reichardt LF (1997) Integrin alpha8beta1 is critically important for epithelial-mesenchymal interactions during kidney morphogenesis. Cell 88:603–613
Muro AF, Chauhan AK, Gajovic S, Iaconcig A, Porro F, Stanta G, Baralle FE (2003) Regulated splicing of the fibronectin EDA exon is essential for proper skin wound healing and normal lifespan. J Cell Biol 162:149–160
Ni H, Yuen PS, Papalia JM, Trevithick JE, Sakai T, Fassler R, Hynes RO, Wagner DD (2003) Plasma fibronectin promotes thrombus growth and stability in injured arterioles. Proc Natl Acad Sci USA 100:2415–2419
Nishimura SL, Sheppard D, Pytela R (1994) Integrin alpha v beta 8. Interaction with vitronectin and functional divergence of the beta 8 cytoplasmic domain. J Biol Chem 269:28708–28715
Nishiuchi R, Takagi J, Hayashi M, Ido H, Yagi Y, Sanzen N, Tsuji T, Yamada M, Sekiguchi K (2006) Ligand-binding specificities of laminin-binding integrins: a comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins. Matrix Biol 25:189–197
Nomizu M, Kuratomi Y, Malinda KM, Song SY, Miyoshi K, Otaka A, Powell SK, Hoffman MP, Kleinman HK, Yamada Y (1998) Cell binding sequences in mouse laminin alpha1 chain. J Biol Chem 273:32491–32499
O’Leary JM, Hamilton JM, Deane CM, Valeyev NV, Sandell LJ, Downing AK (2004) Solution structure and dynamics of a prototypical chordin-like cysteine-rich repeat (von Willebrand Factor type C module) from collagen IIA. J Biol Chem 279:53857–53866
Obara M, Kang MS, Yamada KM (1988) Site-directed mutagenesis of the cell-binding domain of human fibronectin: separable, synergistic sites mediate adhesive function. Cell 53:649–657
Obara M, Sakuma T, Fujikawa K (2010) The third type III module of human fibronectin mediates cell adhesion and migration. J Biochem 147:327–335
Ohashi T, Erickson HP (2005) Domain unfolding plays a role in superfibronectin formation. J Biol Chem 280:39143–39151
Olden K, Pratt RM, Yamada KM (1979) Role of carbohydrate in biological function of the adhesive glycoprotein fibronectin. Proc Natl Acad Sci USA 76:3343–3347
Orend G, Chiquet-Ehrismann R (2000) Adhesion modulation by antiadhesive molecules of the extracellular matrix. Exp Cell Res 261:104–110
Ozhogina OA, Trexler M, Banyai L, Llinas M, Patthy L (2001) Origin of fibronectin type II (FN2) modules: structural analyses of distantly-related members of the kringle family idey the kringle domain of neurotrypsin as a potential link between FN2 domains and kringles. Protein Sci 10:2114–2122
Pankov R, Yamada KM (2002) Fibronectin at a glance. J Cell Sci 115:3861–3863
Patarroyo M, Tryggvason K, Virtanen I (2002) Laminin isoforms in tumor invasion, angiogenesis and metastasis. Semin Cancer Biol 12:197–207
Paul JI, Hynes RO (1984) Multiple fibronectin subunits and their post-translational modifications. J Biol Chem 259:13477–13487
Pickford AR, Potts JR, Bright JR, Phan I, Campbell ID (1997) Solution structure of a type 2 module from fibronectin: implications for the structure and function of the gelatin-binding domain. Structure 5:359–370
Pickford AR, Smith SP, Staunton D, Boyd J, Campbell ID (2001) The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding. EMBO J 20: 1519–1529
Pierschbacher MD, Ruoslahti E (1984) Cell attachment activity of fibronectin can be duplicated by small synthetic fragments of the molecule. Nature 309:30–33
Poschl E, Mayer U, Stetefeld J, Baumgartner R, Holak TA, Huber R, Timpl R (1996) Site-directed mutagenesis and structural interpretation of the nidogen binding site of the laminin gamma1 chain. EMBO J 15:5154–5159
Potts JR, Bright JR, Bolton D, Pickford AR, Campbell ID (1999) Solution structure of the N-terminal F1 module pair from human fibronectin. Biochemistry 38:8304–8312
Preissner KT (1991) Structure and biological role of vitronectin. Annu Rev Cell Biol 7:275–310
Prieto AL, Edelman GM, Crossin KL (1993) Multiple integrins mediate cell attachment to cytotactin/tenascin. Proc Natl Acad Sci USA 90:10154–10158
Reinhardt D, Mann K, Nischt R, Fox JW, Chu ML, Krieg T, Timpl R (1993) Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc. J Biol Chem 268:10881–10887
Rozzo C, Ratti P, Ponzoni M, Cornaglia-Ferraris P (1993) Modulation of alpha 1 beta 1, alpha 2 beta 1 and alpha 3 beta 1 integrin heterodimers during human neuroblastoma cell differentiation. FEBS Lett 332:263–267
Ruoslahti E (2003) The RGD story: a personal account. Matrix Biol 22:459–465
Ruoslahti E, Engvall E, Hayman EG, Spiro RG (1981) Comparative studies on amniotic fluid and plasma fibronectins. Biochem J 193:295–299
Salmivirta K, Talts JF, Olsson M, Sasaki T, Timpl R, Ekblom P (2002) Binding of mouse nidogen-2 to basement membrane components and cells and its expression in embryonic and adult tissues suggest complementary functions of the two nidogens. Exp Cell Res 279:188–201
Sasaki T, Gohring W, Pan TC, Chu ML, Timpl R (1995) Binding of mouse and human fibulin-2 to extracellular matrix ligands. J Mol Biol 254:892–899
Sato Y, Uemura T, Morimitsu K, Sato-Nishiuchi R, Manabe R, Takagi J, Yamada M, Sekiguchi K (2009) Molecular basis of the recognition of nephronectin by integrin alpha8beta1. J Biol Chem 284:14524–14536
Savill J, Dransfield I, Hogg N, Haslett C (1990) Vitronectin receptor-mediated phagocytosis of cells undergoing apoptosis. Nature 343:170–173
Scarborough RM, Naughton MA, Teng W, Rose JW, Phillips DR, Nannizzi L, Arfsten A, Campbell AM, Charo IF (1993) Design of potent and specific integrin antagonists. Peptide antagonists with high specificity for glycoprotein IIb-IIIa. J Biol Chem 268:1066–1073
Scheele S, Nystrom A, Durbeej M, Talts JF, Ekblom M, Ekblom P (2007) Laminin isoforms in development and disease. J Mol Med 85:825–836
Schnapp LM, Hatch N, Ramos DM, Klimanskaya IV, Sheppard D, Pytela R (1995) The human integrin alpha 8 beta 1 functions as a receptor for tenascin, fibronectin, and vitronectin. J Biol Chem 270:23196–23202
Schvartz I, Seger D, Shaltiel S (1999) Vitronectin. Int J Biochem Cell Biol 31:539–544
Schwartz MA, Assoian RK (2001) Integrins and cell proliferation: regulation of cyclin-dependent kinases via cytoplasmic signaling pathways. J Cell Sci 114:2553–2560
Schwarz-Linek U, Hook M, Potts JR (2004) The molecular basis of fibronectin-mediated bacterial adherence to host cells. Mol Microbiol 52:631–641
Schwarzbauer JE (1991) Identification of the fibronectin sequences required for assembly of a fibrillar matrix. J Cell Biol 113:1463–1473
Sechler JL, Corbett SA, Schwarzbauer JE (1997) Modulatory roles for integrin activation and the synergy site of fibronectin during matrix assembly. Mol Biol Cell 8:2563–2573
Sechler JL, Cumiskey AM, Gazzola DM, Schwarzbauer JE (2000) A novel RGD-independent fibronectin assembly pathway initiated by alpha4beta1 integrin binding to the alternatively spliced V region. J Cell Sci 113(Pt 8):1491–1498
Seiffert D, Crain K, Wagner NV, Loskutoff DJ (1994) Vitronectin gene expression in vivo. Evidence for extrahepatic synthesis and acute phase regulation. J Biol Chem 269:19836–19842
Sharma A, Askari JA, Humphries MJ, Jones EY, Stuart DI (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin. EMBO J 18:1468–1479
Shinde AV, Bystroff C, Wang C, Vogelezang MG, Vincent PA, Hynes RO, Van De Water L (2008) Identification of the peptide sequences within the EIIIA (EDA) segment of fibronectin that mediate integrin alpha9beta1-dependent cellular activities. J Biol Chem 283:2858–2870
Skorstengaard K, Thogersen HC, Petersen TE (1984) Complete primary structure of the collagen-binding domain of bovine fibronectin. Eur J Biochem 140:235–243
Skorstengaard K, Thogersen HC, Vibe-Pedersen K, Petersen TE, Magnusson S (1982) Purification of twelve cyanogen bromide fragments from bovine plasma fibronectin and the amino acid sequence of eight of them. Overlap evidence aligning two plasmic fragments, internal homology in gelatin-binding region and phosphorylation site near C terminus. Eur J Biochem 128:605–623
Smith JW, Vestal DJ, Irwin SV, Burke TA, Cheresh DA (1990) Purification and functional characterization of integrin alpha v beta 5. An adhesion receptor for vitronectin. J Biol Chem 265:11008–11013
Sottile J, Schwarzbauer J, Selegue J, Mosher DF (1991) Five type I modules of fibronectin form a functional unit that binds to fibroblasts and Staphylococcus aureus. J Biol Chem 266: 12840–12843
Springer TA, Zhu J, Xiao T (2008) Structural basis for distinctive recognition of fibrinogen gammaC peptide by the platelet integrin alphaIIbbeta3. J Cell Biol 182:791–800
Sriramarao P, Mendler M, Bourdon MA (1993) Endothelial cell attachment and spreading on human tenascin is mediated by alpha 2 beta 1 and alpha v beta 3 integrins. J Cell Sci 105(Pt 4): 1001–1012
Stetefeld J, Mayer U, Timpl R, Huber R (1996) Crystal structure of three consecutive laminin-type epidermal growth factor-like (LE) modules of laminin gamma1 chain harboring the nidogen binding site. J Mol Biol 257:644–657
Sun X, Skorstengaard K, Mosher DF (1992) Disulfides modulate RGD-inhibitable cell adhesive activity of thrombospondin. J Cell Biol 118:693–701
Takahashi S, Leiss M, Moser M, Ohashi T, Kitao T, Heckmann D, Pfeifer A, Kessler H, Takagi J, Erickson HP, Fassler R (2007) The RGD motif in fibronectin is essential for development but dispensable for fibril assembly. J Cell Biol 178:167–178
Thiagarajan P, Kelly K (1988) Interaction of thrombin-stimulated platelets with vitronectin (S-protein of complement) substrate: inhibition by a monoclonal antibody to glycoprotein IIb–IIIa complex. Thromb Haemost 60:514–517
Timpl R (1989) Structure and biological activity of basement membrane proteins. Eur J Biochem 180:487–502
Timpl R, Brown JC (1996) Supramolecular assembly of basement membranes. Bioessays 18: 123–132
Timpl R, Tisi D, Talts JF, Andac Z, Sasaki T, Hohenester E (2000) Structure and function of laminin LG modules. Matrix Biol 19:309–317
Tomasini BR, Mosher DF (1991) Vitronectin. Prog Hemost Thromb 10:269–305
Tomasini-Johansson BR, Annis DS, Mosher DF (2006) The N-terminal 70-kDa fragment of fibronectin binds to cell surface fibronectin assembly sites in the absence of intact fibronectin. Matrix Biol 25:282–293
Tucker RP, Chiquet-Ehrismann R (2009) Evidence for the evolution of tenascin and fibronectin early in the chordate lineage. Int J Biochem Cell Biol 41:424–434
Tzu J, Marinkovich MP (2008) Bridging structure with function: structural, regulatory, and developmental role of laminins. Int J Biochem Cell Biol 40:199–214
Vogel T, Guo NH, Krutzsch HC, Blake DA, Hartman J, Mendelovitz S, Panet A, Roberts DD (1993) Modulation of endothelial cell proliferation, adhesion, and motility by recombinant heparin-binding domain and synthetic peptides from the type I repeats of thrombospondin. J Cell Biochem 53:74–84
Wagner TE, Frevert CW, Herzog EL, Schnapp LM (2003) Expression of the integrin subunit alpha8 in murine lung development. J Histochem Cytochem 51:1307–1315
Watt FM (2002) Role of integrins in regulating epidermal adhesion, growth and differentiation. EMBO J 21:3919–3926
Wayner EA, Garcia-Pardo A, Humphries MJ, McDonald JA, Carter WG (1989) Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J Cell Biol 109:1321–1330
Weeks BS, DiSalvo J, Kleinman HK (1990) Laminin-mediated process formation in neuronal cells involves protein dephosphorylation. J Neurosci Res 27:418–426
Weeks BS, Papadopoulos V, Dym M, Kleinman HK (1991) cAMP promotes branching of laminin-induced neuronal processes. J Cell Physiol 147:62–67
Wei Y, Waltz DA, Rao N, Drummond RJ, Rosenberg S, Chapman HA (1994) Identification of the urokinase receptor as an adhesion receptor for vitronectin. J Biol Chem 269:32380–32388
Wennerberg K, Lohikangas L, Gullberg D, Pfaff M, Johansson S, Fassler R (1996) Beta 1 integrin-dependent and -independent polymerization of fibronectin. J Cell Biol 132:227–238
White DP, Caswell PT, Norman JC (2007) alpha v beta3 and alpha5beta1 integrin recycling pathways dictate downstream Rho kinase signaling to regulate persistent cell migration. J Cell Biol 177:515–525
Williams MJ, Phan I, Harvey TS, Rostagno A, Gold LI, Campbell ID (1994) Solution structure of a pair of fibronectin type 1 modules with fibrin binding activity. J Mol Biol 235:1302–1311
Woods A, Couchman JR, Johansson S, Hook M (1986) Adhesion and cytoskeletal organisation of fibroblasts in response to fibronectin fragments. EMBO J 5:665–670
Wu C, Chung AE, McDonald JA (1995a) A novel role for alpha 3 beta 1 integrins in extracellular matrix assembly. J Cell Sci 108(Pt 6):2511–2523
Wu C, Keivens VM, O’Toole TE, McDonald JA, Ginsberg MH (1995b) Integrin activation and cytoskeletal interaction are essential for the assembly of a fibronectin matrix. Cell 83:715–724
Xiong JP, Stehle T, Zhang R, Joachimiak A, Frech M, Goodman SL, Arnaout MA (2002) Crystal structure of the extracellular segment of integrin alpha Vbeta3 in complex with an Arg-Gly-Asp ligand. Science 296:151–155
Xu J, Bae E, Zhang Q, Annis DS, Erickson HP, Mosher DF (2009) Display of cell surface sites for fibronectin assembly is modulated by cell adherence to 1F3 and C-terminal modules of fibronectin. PLoS ONE 4:e4113
Yamada KM, Kennedy DW, Kimata K, Pratt RM (1980) Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. J Biol Chem 255:6055–6063
Yang JT, Hynes RO (1996) Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins. Mol Biol Cell 7:1737–1748
Yi XY, Wayner EA, Kim Y, Fish AJ (1998) Adhesion of cultured human kidney mesangial cells to native entactin: role of integrin receptors. Cell Adhes Commun 5:237–248
Yokosaki Y, Matsuura N, Higashiyama S, Murakami I, Obara M, Yamakido M, Shigeto N, Chen J, Sheppard D (1998) Identification of the ligand binding site for the integrin alpha9 beta1 in the third fibronectin type III repeat of tenascin-C. J Biol Chem 273:11423–11428
Yurchenco PD, Schittny JC (1990) Molecular architecture of basement membranes. FASEB J 4:1577–1590
Yurchenco PD, Wadsworth WG (2004) Assembly and tissue functions of early embryonic laminins and netrins. Curr Opin Cell Biol 16:572–579
Zaidel-Bar R, Itzkovitz S, Ma’ayan A, Iyengar R, Geiger B (2007) Functional atlas of the integrin adhesome. Nat Cell Biol 9:858–867
Zhang Q, Checovich WJ, Peters DM, Albrecht RM, Mosher DF (1994) Modulation of cell surface fibronectin assembly sites by lysophosphatidic acid. J Cell Biol 127:1447–1459
Zhang Q, Magnusson MK, Mosher DF (1997) Lysophosphatidic acid and microtubule-destabilizing agents stimulate fibronectin matrix assembly through Rho-dependent actin stress fiber formation and cell contraction. Mol Biol Cell 8:1415–1425
Zhang WM, Kapyla J, Puranen JS, Knight CG, Tiger CF, Pentikainen OT, Johnson MS, Farndale RW, Heino J, Gullberg D (2003) alpha 11beta 1 integrin recognizes the GFOGER sequence in interstitial collagens. J Biol Chem 278:7270–7277
Zheng X, Saunders TL, Camper SA, Samuelson LC, Ginsburg D (1995) Vitronectin is not essential for normal mammalian development and fertility. Proc Natl Acad Sci USA 92: 12426–12430
Zhong C, Chrzanowska-Wodnicka M, Brown J, Shaub A, Belkin AM, Burridge K (1998) Rho-mediated contractility exposes a cryptic site in fibronectin and induces fibronectin matrix assembly. J Cell Biol 141:539–551
Zhou A, Huntington JA, Pannu NS, Carrell RW, Read RJ (2003) How vitronectin binds PAI-1 to modulate fibrinolysis and cell migration. Nat Struct Biol 10:541–544
Zhou X, Rowe RG, Hiraoka N, George JP, Wirtz D, Mosher DF, Virtanen I, Chernousov MA, Weiss SJ (2008) Fibronectin fibrillogenesis regulates three-dimensional neovessel formation. Genes Dev 22:1231–1243
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Berlin Heidelberg
About this chapter
Cite this chapter
Xu, J., Mosher, D. (2011). Fibronectin and Other Adhesive Glycoproteins. In: Mecham, R. (eds) The Extracellular Matrix: an Overview. Biology of Extracellular Matrix. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16555-9_2
Download citation
DOI: https://doi.org/10.1007/978-3-642-16555-9_2
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-16554-2
Online ISBN: 978-3-642-16555-9
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)