Abstract
Serrawettins are nonionic biosurfactants produced by Serratia marcescens. Three molecular species, serrawettin W1, cyclo(d-3-hydroxydecanoyl-l-seryl)2; W2, d-3-hydroxydecanoyl-d-leucyl-l-seryl-l-threonyl-d-phenylalanyl-l-isoleucyl lactone; and W3, cyclodepsipeptide composed of five amino acids and one dodecanoic acid, have been reported. Serratia rubidaea produces rubiwettin R1, linked d-3-hydroxy fatty acids and RG1, β-glucopyranosyl linked d-3-hydroxy fatty acids. These biosurfactants are produced mainly at 30°C, but not at 37°C, and secreted through extracellular vesicles on solid media. The contribution of the biosurfactants to spreading growth in surface environments has been determined, and it is prominent under nutrient-poor conditions. Analyses of S. marcescens mutants revealed the involvement of three novel genes for serrawettin W1 production. The gene pswP encodes a phosphopantetheinyl transferase group enzyme, swrW encodes a unimodular synthetase belonging to the nonribosomal peptide synthetase (NRPS) family, and hexS encodes a LysR-type transcriptional regulator working as a downregulator of Serratia exolipids and some exoenzymes. Autoinducer-dependent serrawettin W2 production has been elucidated by the finding of SwrI/SwrR (homolog of LuxI/LuxR) and N-acyl homoserine lactones in the study on quorum-sensing controlled-swarming of S. marcescens.
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Acknowledgments
The authors are indebted to all their coworkers at Niigata University and Chuo University. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and by a grant from the Urakami Foundation.
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Matsuyama, T., Tanikawa, T., Nakagawa, Y. (2011). Serrawettins and Other Surfactants Produced by Serratia . In: Soberón-Chávez, G. (eds) Biosurfactants. Microbiology Monographs, vol 20. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-14490-5_4
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