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Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64A

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Progress in Botany 71

Part of the book series: Progress in Botany ((BOTANY,volume 71))

Abstract

Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals <365 protein-encoding genes and 11 tRNA genes. Products of about 40% of these genes resemble proteins of known function, including many that are unexpected for a virus. Among these is a virus-encoded protein, called Kcv, which forms a functional K+ channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K+ channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.

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Notes

  1. 1.

    The contact angle α describes the orientation of the membrane plane with respect to the inner lining of the small hole in the cell wall. An angle of 0o means that the plane of the membrane is quasi parallel to the wall; e.g. the membrane reveals an omega shape bulge. If at the other extreme the angle is 90o the membrane is perpendicular to the wall; the membrane remains flat in the plane without any budging out.

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Acknowledgments

The authors are grateful for financial support for this research by the Deutsche Forschungsgemeinschaft to GT and the European Drug Initiative on Channels and Transporters (EDICT) project EU FP7 (201924) to A.M. Research in the Van Etten laboratory was supported in part by Public Health Service grant GM32441 from the National Institute of General Medical Sciences and NIH grant P20-RR1565 from the COBRE program of the National Center for Research Resources.

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Authors and Affiliations

  1. Institute of Botany, Technische Universität Darmstadt, 64287, Darmstadt, Germany

    Gerhard Thiel

  2. Dipartimento di Biologia, Università degli Studi di Milano, E Istituto di Biofisica, CNR, U-Milano, 20133, Milano, Italy

    Anna Moroni

  3. Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska, Lincoln, NE, 68583-0722, USA

    David Dunigan & James L. Van Etten

Authors
  1. Gerhard Thiel
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  2. Anna Moroni
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  3. David Dunigan
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  4. James L. Van Etten
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Corresponding author

Correspondence to Gerhard Thiel .

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Editors and Affiliations

  1. Inst. Botanik, TU Darmstadt, Schnittspahnstr. 3-5, Darmstadt, 64287, Germany

    Ulrich Lüttge

  2. Fakultät für Biologie, LS für Experimentelle Ökologie, Universität Bielefeld, Bielefeld, Germany

    Wolfram Beyschlag

  3. FB Biologie, Abt. Allgemeine Botanik, TU Kaiserslautern, Erwin-Schrödinger-Str., Kaiserslautern, 67663, Germany

    Burkhard Büdel

  4. Cardiff School of Biosciences, University of Cardiff, Cardiff, United Kingdom

    Dennis Francis

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Thiel, G., Moroni, A., Dunigan, D., Van Etten, J.L. (2010). Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64A. In: Lüttge, U., Beyschlag, W., Büdel, B., Francis, D. (eds) Progress in Botany 71. Progress in Botany, vol 71. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-02167-1_7

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