The enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used technique in modern diagnostics. A modification of the standard ELISA is the competitive ELISA (cELISA). It is a suitable diagnostic tool for seroepidemiological surveillance in human and veterinary medicine and it is an established system for diagnostic of a multiplicity of pathogens. An advantage to other diagnostic methods is species independency, since the detection system detects the generic monoclonal antibody used for competition. For the same reason false positive results caused by cross-reactions with closely related pathogens are also reduced.
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References
Afshar A et al (1987) Comparison of competitive and indirect enzyme-linked immunosorbent assays for detection of bluetongue virus antibodies in serum and whole blood. J Clin Microbiol 25(9):1705–1710
Afshar A, Shakarchi NH, Dulac GC (1993) Development of a competitive enzyme-linked immunosorbent assay for detection of bovine, ovine, porcine, and equine antibodies to vesicular stomatitis virus. J Clin Microbiol 31(7):1860–1865
Engvall E, Perlmann P (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8(9):871–874
Mecham JO, Jochim MM (2000) Development of an enzyme-linked immunosorbent assay for the detection of antibody to epizootic hemorrhagic disease of deer virus. J Vet Diagn Invest 12(2):142–145 Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Mecham JO, Wilson WC (2004) Antigen capture competitive enzyme-linked immunosorbent assays using baculovirus-expressed antigens for diagnosis of bluetongue virus and epizootic hemorrhagic disease virus. J Clin Microbiol 42(2):518–523
Renukaradhya GJ et al (2003) Competitive enzyme-linked immunosorbent assay based on monoclonal antibody and recombinant hemagglutinin for serosurveillance of rinderpest virus. J Clin Microbiol 41(3):943–947
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Meyer, T. (2010). Competitive ELISA. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Protocols Handbooks. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-01144-3_47
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DOI: https://doi.org/10.1007/978-3-642-01144-3_47
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