Abstract
3D-stacks of optical sections through the vertebrate retina with fluorescent stained cell nuclei were measured with a laser scanning microscope. The evaluation of the data volumes with dedicated digital imaging algorithms gives access to complex morphometric tissue-characters that are discussed in terms of functional morphology. The thickness of nuclear layers and the 3D-coordinates of cell nuclei are detected automatically to measure cell densities, cell ratios and to create character-distribution-maps of the entire retina.
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Heß, M. (2007). Semi-automated Mapping of Cell Nuclei in 3D-Stacks from Optical-Sectioning Microscopy. In: Perner, P., Salvetti, O. (eds) Advances in Mass Data Analysis of Signals and Images in Medicine, Biotechnology and Chemistry. MDA 2007. Lecture Notes in Computer Science(), vol 4826. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-76300-0_16
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DOI: https://doi.org/10.1007/978-3-540-76300-0_16
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-76299-7
Online ISBN: 978-3-540-76300-0
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