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Characteristics of Mammalian Cells and Requirements for Cultivation

  • Chapter
Cell and Tissue Reaction Engineering

Part of the book series: Principles and Practice ((PRINCIPLES))

Abstract

This chapter aims at presenting a general overview on the specific characteristics of mammalian cells to those not familiar with cell biology. At first, differences between mammalian cells, plant cells and microbes are discussed. Then consequences for design of bioreactors and processes are also discussed. Different types of mammalian cells (primary cells, permanent (established) cell lines, hybridom cells) are introduced. Techniques required, to get from primary cells to permanent (established) cell lines and the hybridom technique for production of monoclonal antibodies are also briefly outlined. Culture collections and cell banking are discussed with respect to its purpose and the build-up. Appropriate culture media is an essential requirement for successful mammalian cell culture and is therefore introduced in depth. The chapter also focuses on characteristics of cell growth and metabolism. A short introduction to cell metabolism is followed by a detailed discussion on aspects related to modelling of cell growth and metabolism of mammalian cells.

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Abbreviations

c P :

product concentration

c S :

substrate concentration

d 0,d 1 :

fit parameter

i :

index for substrate

j :

index for product

k d :

cell specific death rate

k d,max :

maximal cell specific death rate

k d,min :

minimal cell specific death rate

k d,P :

kinetic constant for effect of metabolite on cell death

k d,S :

kinetic constant for effect of substrate on cell death

k p :

metabolite inhibition constant

k s :

substrate limitation constant

m:

maintenance term

NH3:

index for undissoziated ammonia

q P :

cell specific production rate

q S :

cell specific substrate uptake rate

q S,max :

maximal cell specific substrate uptake rate

Y x/s :

yield coefficient cell number per mol substrate

ÎĽ:

cell specific growth rate

ÎĽmax :

maximal cell specific growth rate

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  1. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Denickestr. 15, D-21073, Hamburg, Germany

    R. Pörtner

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© 2009 Springer-Verlag Berlin Heidelberg

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Pörtner, R. (2009). Characteristics of Mammalian Cells and Requirements for Cultivation. In: Cell and Tissue Reaction Engineering. Principles and Practice. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-68182-3_2

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