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Cre/loxP-Mediated Chromosome Engineering of the Mouse Genome

  • Chapter
Conditional Mutagenesis: An Approach to Disease Models

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 178))

Abstract

Together with numerous other genome modifications, chromosome engineering offers a very powerful tool to accelerate the functional analysis of the mammalian genome. The technology, based on the Cre/loxP system, is used more and more in the scientific community in order to generate new chromosomes carrying deletions, duplications, inversions and translocations in targeted regions of interest. In this review, we will present the basic principle of the technique either in vivo or in vitro and we will briefly describe some applications to provide highly valuable genetic tools, to decipher the mammalian genome organisation and to analyze human diseases in the mouse.

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Authors and Affiliations

  1. Institut de Transgénose, IEM, UMR6812, CNRS Uni-Orléans, 3B rue de la Férollerie, 45071, Orléans Cedex 2, France

    V. Brault, V. Besson, L. Magnol, A. Duchon & Y. Hérault

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  1. V. Brault
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  2. V. Besson
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  3. L. Magnol
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  4. A. Duchon
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  5. Y. Hérault
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Editors and Affiliations

  1. Interfakultäres Institut für Biochemie (IFIB) Signaltransduktion — transgene Modelle, Universität Tübingen, Hoppe-Seyler-Str. 4, 72076, Tübingen, Germany

    Robert Feil

  2. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), 1, rue Laurent Fries, 67404, Illkirch, France

    Daniel Metzger

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Brault, V., Besson, V., Magnol, L., Duchon, A., Hérault, Y. (2007). Cre/loxP-Mediated Chromosome Engineering of the Mouse Genome. In: Feil, R., Metzger, D. (eds) Conditional Mutagenesis: An Approach to Disease Models. Handbook of Experimental Pharmacology, vol 178. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-35109-2_2

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