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Glycomics and Mass Spectrometry

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Glycoscience

Abstract

There is an increasing body of evidence indicating that glycans are implicated in numerous biological processes such as cell–cell interactions, intracellular signaling, and immune response. Glycomics emerges from the necessity to understand the mechanisms underlying the interactions responsible for these activities. The term glycomics is used to describe experimental approaches to studying the structure and function of the glycomes of fluids, cells, tissues, organs etc. Glycomics embraces a variety of technologies amongst which mass spectrometry (MS) plays a pivotal role because it is the method of choice for defining the primary structures of glycopolymers. This chapter provides an insight into MS ‑based structural strategies that are best suited to studying complex glycomes.

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Abbreviations

Asn:

asparagine

CAD:

collisionally activated decomposition

CAD ESI‑MS/MS:

collisionally activated decomposition tandem MS

CID:

collisionally induced decomposition

CHCA:

α‑cyano‑4‑hydroxycinnamic acid

Da:

Dalton

DE:

delayed extraction

DHB:

2,5‑dihydroxybenzoic acid / gentisic acid

ECD:

electron capture dissociation

EI:

electron impact/ionization

ESI:

electrospray ionization

ESI‑MS:

electrospray ionization mass spectrometry

eV:

electron volt

FAB:

fast atom bombardment

FAB-MS:

fast atom bombardment mass spectrometry

FT-ICR:

Fourier transform ion cyclotron resonance

Fuc:

fucose

Gal:

galactose

GalNAc:

N‑acetylgalactosamine

GC-MS:

gaschromatography–mass spectrometry

Glc:

glucose

GlcNAc:

N-acetylglucosamine

Hex:

hexose

HexNAc:

N-acetylhexosamine

HF:

hydrofluoric acid

Hz:

Hertz

kDa:

kilo-Dalton

kV:

kilo-Volt

LC:

liquid chromatography

LC (ESI)-MS:

liquid chromatography (electrospray ionization) mass spectrometry

m/z :

mass / charge

MALDI:

matrix‐assisted laser desorption ionization

MALDI-MS:

matrix‐assisted laser desorption ionization mass spectrometry

MALDI-TOFMS:

matrix‐assisted laser desorption ionization–time-of-flight mass spectrometry

Man:

mannose

MS:

mass spectrometry

MS/MS:

tandem mass spectrometry

MSn :

multiple-stage mass spectrometry

µl:

micro-Liter

NeuAc:

N-acetylneuraminic acid

NeuGc:

N-glycolylneuraminic acid

Nd-YAG:

neodymium-doped yttrium aluminum garnet

PNGase F:

peptide: N‑glycosidase F / N‑glycanase

ppm:

part per million

Q-TOF:

quadrupole/orthogonal acceleration time-of-flight

Ser:

serine

TFA:

trifluoroacetic acid

Thr:

threonine

TOF:

time-of-flight

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Author information

Authors and Affiliations

  1. Biopolymer Mass Spectrometry Group, Division of Molecular Biosciences, Imperial College London, SW7 2AZ, London, UK

    Anne Dell, Jihye Jang-Lee, Poh-Choo Pang, Simon Parry, Mark Sutton-Smith, Berangere Tissot, Howard R. Morris, Maria Panico & Stuart M. Haslam

Authors
  1. Anne Dell
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  2. Jihye Jang-Lee
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  3. Poh-Choo Pang
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  4. Simon Parry
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  5. Mark Sutton-Smith
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  6. Berangere Tissot
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  7. Howard R. Morris
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  8. Maria Panico
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  9. Stuart M. Haslam
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Editor information

Editors and Affiliations

  1. Natural Products and Glycotechnology Research Institute Inc., North Caroline State University, 4118 Swarthmore Road, Durham, NC, 27707, USA

    Bertram O. Fraser-Reid Prof. Dr.

  2. University Graduate School of Science and Engineering, 3-4-1 Ohkubo, Shinjuku, Tokyo, Japan

    Kuniaki Tatsuta Prof. Dr.

  3. Faculty of ScienceDepartment of Chemistry, University of Hamburg, Martin-Luther-King-Platz 6, 20146, Hamburg, Germany

    Joachim Thiem Prof. Dr.

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© 2008 Springer-Verlag Berlin Heidelberg New York

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Dell, A. et al. (2008). Glycomics and Mass Spectrometry . In: Fraser-Reid, B.O., Tatsuta, K., Thiem, J. (eds) Glycoscience. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-540-30429-6_56

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