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Analysis of Nucleic Acids by Capillary Electrophoresis

  • Book
  • © 1997

Overview

Editors:
  1. Christoph Heller

    1. Max-Planck-Institut für molekulare Genetik, Berlin, Germany

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  • Aktuelle Wissenschaft für Praktiker

Part of the book series: CHROMATOGRAPHIA CE-Series (CHROM, volume 1)

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Table of contents (13 chapters)

  1. Front Matter

    Pages I-X
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  2. Basic Concepts

    1. Front Matter

      Pages 1-1
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    2. The Separation Matrix

      • Christoph Heller
      Pages 3-23

    3. Electrophoresis Theories

      • Gary W. Slater
      Pages 24-66

    4. Microscopic Studies on the Migration Mechanism

      • Christina Carlsson, Anette Larsson, Mats Jonsson
      Pages 67-89

  3. Factors Affecting the Separation

    1. Front Matter

      Pages 91-91
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    2. Influence of Polymer Concentration and Electric Field Experimental Study and Comparison with Theory

      • Annelise E. Barron, Christoph Heller
      Pages 93-124

    3. Effects of Sample Matrix and Injection

      • John M. Butler
      Pages 125-134

    4. Developments in Capillary Coating and DNA Separation Matrices

      • Marcella Chiari, Arianna Gelain
      Pages 135-173

  4. Applications

    1. Front Matter

      Pages 175-175
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    2. Analysis of Oligonucleotides and Antisense DNA Analogs

      • Aran Paulus, Gerard J. M. Bruin, Iris Barmé, Angelika Muscate, Markus Ehrat
      Pages 177-194

    3. Separation of DNA Restriction Fragments and PCR Products

      • John M. Butler
      Pages 195-217

    4. Pulsed-Field Capillary Electrophoresis of Large DNA

      • Jan Sudor, Milos V. Novotny
      Pages 218-235

    5. Capillary Electrophoresis for DNA Sequencing

      • Norman J. Dovichi
      Pages 236-254

    6. Mutational Analysis with Capillary Electrophoresis

      • Pier Giorgio Righetti, Cecilia Gelfi
      Pages 255-273

    7. Analysis of Transfer RNA and 5S Ribosomal RNA

      • Eleftheria Katsivela
      Pages 274-293

  5. Recent Developments

    1. Front Matter

      Pages 295-295
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    2. Update on Improvements in DNA Separation

      • Christoph Heller
      Pages 297-308

  6. Back Matter

    Pages 309-314
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Keywords

About this book

During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be­ come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe­ cialization is unavoidable. This evolution is also reflected in the development of instrumen­ tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.

Editors and Affiliations

  • Max-Planck-Institut für molekulare Genetik, Berlin, Germany

    Christoph Heller

About the editor

Dr. Christoph Heller ist Wissenschaftler am Max-Planck-Institut für molekulare Genetik in Berlin.

The editor, Dr. Christoph Heller, is currently working at the Max-Planck-Institute for Molecular Genetics in Berlin-Dahlem, Germany. Since 1985 he is working in the field of DNA separation, both in conventional gels and - since five years - also in capillaries.

Bibliographic Information

  • Book Title: Analysis of Nucleic Acids by Capillary Electrophoresis

  • Editors: Christoph Heller

  • Series Title: CHROMATOGRAPHIA CE-Series

  • DOI: https://doi.org/10.1007/978-3-322-91015-8

  • Publisher: Vieweg+Teubner Verlag Wiesbaden

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Fachmedien Wiesbaden 1997

  • Softcover ISBN: 978-3-322-91017-2Published: 06 January 2013

  • eBook ISBN: 978-3-322-91015-8Published: 29 June 2013

  • Series ISSN: 1615-2255

  • Edition Number: 1

  • Number of Pages: X, 314

  • Topics: Engineering, general

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