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Radioimmunoassay (RIA)

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Basic Serological Testing

Abstract

Radioimmunoassay (RIA) quantifies the amount of specific antigens in patient serum. This technique uses antigens labelled with radioisotopes, usually 125I, which requires specific safety measures; therefore, the use of RIA is very rare in clinical laboratories, especially with the presence of ELISA, which replaces the radioisotopes with enzyme. However, this chapter describes the principle, reagents, steps, and results interpretation of RIA.

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References

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Alhabbab, R.Y. (2018). Radioimmunoassay (RIA). In: Basic Serological Testing. Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-319-77694-1_11

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