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Heat Shock Proteins and Pesticide Stress

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Regulation of Heat Shock Protein Responses

Part of the book series: Heat Shock Proteins ((HESP,volume 13))

Abstract

Heat shock proteins (HSP) are highly conserved bio-molecules found almost in all taxa from protozoa to higher vertebrates. HSP mainly function as chaperones to protect proteins from unfolding, aggregation and destruction. They also help to cope with a range of environmental stresses. Pesticides in majority are synthetic chemicals used in agricultural fields to kill pests, but when ingested by non-target organisms, they generate reactive oxygen species and oxidative stress (OS). OS catalyzes unfolding and aggregation of native proteins. In response, HSP are synthesized within cells and block protein degradation. Therefore, HSP are produced to counteract pesticide stress and hence they are designated as potent biomarkers of OS. Several techniques are available that can monitor HSP level following pesticide insult. Reporter gene assay (HSP70-LacZ or HSP70-GFP) is a common practice and it provides qualitative information. Northern and Western Blot analysis presents quantitative measures of HSP at both mRNA and protein levels. Therefore, HSP constitute a part of cell protection machinery as well as they belong to first tier of biomarkers considered in risk assessment of pesticides.

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Acknowledgements

Authors show deep sense of gratitude towards the Head, Department of Zoology (DST-FIST, UGC-DRS sponsored), The University of Burdwan for infrastructural facilities. Continuous support obtained from other members of our research group during writing this book chapter is thankfully acknowledged.

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Authors and Affiliations

  1. Post Graduate Department of Zoology, A. B. N. Seal College, Cooch Behar, West Bengal, India

    Prem Rajak

  2. Toxicology Research Unit, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, India

    Sumedha Roy

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  1. Prem Rajak
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  2. Sumedha Roy
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Editors and Affiliations

  1. Department of Medicine and Precision Therapeutics Proteogenomics Diagnostic Center, Eleanor N. Dana Cancer Center, University of Toledo College of Medicine and Life Sciences, Toledo, Ohio, USA

    Alexzander A A Asea

  2. Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA

    Punit Kaur

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Rajak, P., Roy, S. (2018). Heat Shock Proteins and Pesticide Stress. In: Asea, A., Kaur, P. (eds) Regulation of Heat Shock Protein Responses. Heat Shock Proteins, vol 13. Springer, Cham. https://doi.org/10.1007/978-3-319-74715-6_2

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