Abstract
Quantum dots are nanoparticles, which due to their unique physical and chemical (first of all optical) properties, are promising in biology and medicine. There are many ways for quantum dots synthesis, both in the form of nanoislands self-forming on the surfaces, which can be used as single-photon emitters in electronics for storing information, and in the form of colloidal quantum dots for diagnostic and therapeutic purposes in living systems. The paper describes the main methods of quantum dots synthesis and summarizes medical and biological ways of their use. The main emphasis is laid on the ways of quantum dots surface modification. Influence of the size and form of nanoparticles, charge on the surfaces of quantum dots, and cover type on the efficiency of internalization by cells and cell compartments is shown. The main mechanisms of penetration are considered.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Yashin KD, Osipovich VS, Pitsuk SE (2007) Structure of the nanocrystals of cadmium selenit received by the method of colloid chemistry for the use in medical diagnostics. Rep BGUIR 3:74–79
Guo Z, Tan L (2009) Fundamentals and applications of nanomaterials. Artech House, London, pp 1–249
Musihin SF, Alexandrova OA, Luchinin VV et al (2012) Semiconductor colloidal nanoparticles in biology and medicine. Fund Biomed Eng 5–6:47–55
Rempel SV, Razvodov AA, Nebogatikov MC et al (2013) Sizes and fluorescence of quantum dots of cadmium sulfide. Solid State Phys 55(3):567–571
Zhu JJ, Li JJ, Huang HP et al (2013) Quantum dots for DNA biosensing. Springer Briefs Mol Sci. https://doi.org/10.1007/978-3-642-44910-9-2
Samohvalov PS, Artemyev MV, Nabiev IR (2013) Modern methods of luminescent semiconductor nanocrystals synthesis for biomedical usage. Russ Nanotechnol 8(5–6):119–129
Speranskaya ES, GoryachevaYu I (2013) Fluorescent quantum dots: synthesis, modification and use in the immunoanalysis. Russ Nanotechnol 8(11–12):7–17
Oleynikov VA, Sukhanova AV, Nabiyev IR (2007) Fluorescent semiconductor nanocrystals in biology and medicine. Russ Nanotechnol 2(1–2):160–173
Gao X, Chan WCW, Nie S (2002) Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding. J Biomed Opt 7:532–537
Duran N, Silveira CP, Duran M et al (2015) Silver nanoparticle protein corona and toxicity: mini-review. J Nanobiotechnol 13:1–17
Casals E, Pfaller T, Duschl A et al (2011) Hardening of the nanoparticle-protein corona in metal (Au, Ag) and oxide (Fe3O4, CoO, and CeO2) nanoparticles. Small 7:3479–3486
Kairdolf BA, Smith AM, Stokes TH et al (2013) Semiconductor quantum dots for bioimaging and biodiagnostic applications. Annu Rev Anal Chem 6(1):143–162
Belyaeva TN, Salova AV, Leonteva EA et al (2009) Inappropriate quantum points in vital confocal microscopic examinations of cells. Tsitologiia 51:838–848
Lidke DS, Nagy P, Heintzmann R et al (2004) Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction. Nat Biotechnol 22(2):198–203
Pleskova SN, Balalayev IV, Gushchina YY et al (2009) Distinctions in functional activity of neutrophilic granulocytes in reactions with semiconductor quantum dots. Morphology 3:47–49
Crane JM, Haggie PM, Verkman AS (2009) Quantum dot single molecule tracking reveals a wide range of diffusive motions of membrane transport proteins. Proc SPIE 7489. https://doi.org/10.1117/12.816900
Hanaki K, Momo A, Oku T et al (2003) Semiconductor quantum dot/albumin complex is a long-life and highly photostable endosome marker. Biochem Biophys Res Commun 302:496–501
Lim YT, Kim S, Nakayama A et al (2003) Selection of quantum dot wavelengths for biomedical assays and imaging. Mol Imaging 2(1):50–64
Wu X, Liu H, Liu J et al (2002) Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol 21:41–46
Rhyner MN, Smith AM, Gao XH et al (2006) Quantum dots and multifunctional nanoparticles: new contrast agents for tumor imaging. Nanomedicine 1:209–217
Xing Y, Chaudry Q, Shen C et al (2007) Bioconjugated quantum dots for multiplexed and quantitative immunohistochemistry. Nat Protoc 2:1152–1165
Kim S, Lim YT, Soltesz EG et al (2004) Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. Nat Biotechnol 22:93–97
Gao X, Cui Y, Levenson RM et al (2004) In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 22:969–976
Cai W, Shin DW, Chen K et al (2006) Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects. Nano Lett 6:669–676
Akerman ME, Chan WC, Laakkonen P et al (2002) Nanocrystal targeting in vivo. Proc Natl Acad Sci U S A 99:12617–12621
Yu X, Chen L, Li K et al (2007) Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo. J Biomed Opt 12(1):014008
Zdobnova TA, Lebedenko EN, Deev SM (2011) Quantum dots for molecular diagnostics of tumors. Acta Nat 3(8):30–50
Tada H, Higuchi H, Wanatabe TM et al (2007) In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice. Cancer Res 67:1138–1144
Smith AM, Dave S, Nie S et al (2006) Multicolor quantum dots for molecular diagnostics of cancer. Expert Rev Mol Diagn 6(2):231–244
Parak WJ, Boudreau R, Le Gros M et al (2002) Cell motility and metastatic potential studies based on quantum dot imaging of phagokinetic tracks. Adv Mater 14:882–885
Alivisatos AP, Gu W, Larabell C (2005) Quantum dots as cellular probes. Annu Rev Biomed Eng 7:55–76
Wang J, Wang F, Li F et al (2016) Multifunctional poly(curcumin) nanomedicine for dual-modal targeted delivery, intracellular responsive release, dual-drug treatment and imaging of multidrug resistant cancer cells. J Mater Chem 4:2954–2962
Bagalkot V, Zhang L, Levy-Nissenbaum E et al (2007) Quantum dot-aptamer conjugates for synchronous cancer imaging, therapy, and sensing of drug delivery based on bi-fluorescence resonance energy transfer. Nano Lett 7(10):3065–3070
Samia ACS, Chen X, Burda C (2003) Semiconductor quantum dots for photodynamic therapy. J Am Chem Soc 125(51):15736–15737
Bakalova R, Ohba H, Zhelev Z et al (2004) Quantum dots as photosensitizers? Nat Biotechnol 22(11):1360–1361
Juzenas P, Chen W, Sun YP et al (2008) Quantum dots and nanoparticles for photodynamic and radiation therapies of cancer. Adv Drug Deliv Rev 60:1600–1614
Choi AO, Brown SE, Szyf M et al (2008) Quantum dot-induced epigenetic and genotoxic changes in human breast cancer cells. J Mol Med 86:291–302
Shlyakhto EV (ed) (2009) Nanotechnologies in biology and medicine. Publishing House, St. Petersburg
Kaji N, Tokeshi M, Baba Y (2007) Quantum dots for single bio-molecule imaging. Anal Sci 23:21–24
Hohng S, Ha T (2004) Near-complete suppression of quantum dot blinking in ambient conditions. J Am Chem Soc 126:1324–1325
Chashchin GV, Ponomarev VO, Nosov SV et al (2011) Prospect of use of artificial fluorophor (quantum dots) in ophthalmology. Bull Reg Pub Inst 14:394–396
Chashchin GV, Ponomarev VO, Nosov SV (2012) New inorganic fluorophor and the photosensitive retinal-containing proteins as a basis of element base for quantum nanosurgery of the eye retina. Bull Reg Pub Inst 12:225–228
Nel A, Xia T, Madler L (2006) Toxic potential of materials at the nanolevel. Science 311:622–627
Medina C, Santos-Martinez MJ, Radomski A et al (2007) Nanoparticles: pharmacological and toxicological significance. Br J Pharmacol 150:552–558
Taranova NA, Berlina AN, Zherdeev AV et al (2012) Quantum dota as a marker in immunochromatographic diagnostic test systems. Nanotechnol Health Protect 4:44–47
Ding SG, Chen JX, Jiang HY et al (2006) Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue. J Agric Food Chem 54:6139–6142
Chen YP, Ning BA, Liu N et al (2010) A rapid and sensitive fluoroimmunoassay based on quantum dot for the detection of chlorpyrifos residue in drinking water. J Environ Sci Health B 45:508–515
Tully E, Hearty S, Leonard P et al (2006) The development of rapid fluorescence-based immunoassays, using quantum dot-labeled antibodies for the detection of Listeria monocytogenes cell surface proteins. Int J Biol Macromol 39:127–134
Wang XL, Tao GH, Meng YH (2009) A novel CdSe/CdS quantum dot-based competitive fluoroimmunoassay for the detection of clenbuterol residue in pig urine using magnetic core/shell Fe3O4/Au nanoparticles as a solid carrier. Anal Sci 25:1409–1413
Trapiella-Alfonso L, Costa-Fernandez JM, Pereiro R et al (2011) Development of a quantum dot-based fluorescent immunoassay for progesterone determination in bovine milk. Biosens Bioelectron 26:4753–4759
Korzhevsky E, Kirik OV, Gilyarov AV et al (2010) Use of semiconductor nanocrystals (quantum dots) in immunocytochemistry studies. Morphology 3:71–74
Oleynikov VA (2011) Semiconductor fluorescent nanocrystals (quantum dots) in protein biochips. Bioorg Chem 37(2):171–189
Rejman J, Oberle V, Zuhorn IS et al (2004) Size-dependent internalization of particles via pathways of clatrin-and caveolae-mediated endocytosis. Biochem J 377:159–169
Champion JA, Mitragotri S (2006) Role of target geometry in phagocytosis. Proc Natl Acad Sci U S A 103:4930–4934
Nabiev I, Mitchell S, Davies A et al (2007) Non functionalized nanocrystals can exploit a cell’s active transport machinery delivering them to specific nuclear and cytoplasmic compartments. Nano Lett 7:3452–3461
Lovrić J, Bazzi HS, Cuie Y et al (2005) Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots. J Mol Med 83:377–385
Jiang W, Kim BYS, Rutka JT et al (2008) Nanoparticle-mediated cellular response is size-dependent. Nat Biotechnol 3:145–150
Shan Y, Hao X, Shang X et al (2011) Recording force events of single quantum-dot endocytosis. Chem Commun 47:3377–3379
Hoshino A, Fujioka K, Oku T et al (2004) Quantum dots targeted to the assigned organelle in living cells. Microbiol Immunol 48:985–994
Jaiswal JK, Mattoussi H, Mauro JM et al (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21:47–51
Harush-Frenkel O, Debotton N, Benita S et al (2007) Targeting of nanoparticles to the clathrin-mediated endocytic pathway. Biochem Biophys Res Commun 353:26–32
Khan JA, Pillai B, Das TK et al (2007) Molecular effects of uptake of gold nanoparticles in Hela cells. Chem Biol Chem 8:1237–1240
Xie J, Xu C, Kohler N et al (2007) Controlled PEGylation of monodisperse Fe3O4 nanoparticles for reduced non-specific uptake by macrophage cells. Adv Mater 19:3163–3166
Acknowledgements
This work was supported by the Russian Science Foundation, project â„– 16-14-10179.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 The Author(s)
About this chapter
Cite this chapter
Pleskova, S., Mikheeva, E., Gornostaeva, E. (2018). Using of Quantum Dots in Biology and Medicine. In: Saquib, Q., Faisal, M., Al-Khedhairy, A., Alatar, A. (eds) Cellular and Molecular Toxicology of Nanoparticles. Advances in Experimental Medicine and Biology, vol 1048. Springer, Cham. https://doi.org/10.1007/978-3-319-72041-8_19
Download citation
DOI: https://doi.org/10.1007/978-3-319-72041-8_19
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-72040-1
Online ISBN: 978-3-319-72041-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)