Abstract
The induction of allergic contact dermatitis by chemicals depends on their ability to activate a chemical-specific T cell response. This key event that concludes the skin sensitization process is monitored in an in vitro assay, the human T cell priming assay (hTCPA). Naive human T cells are incubated with autologous dendritic cells pulsed with the chemical of interest. After restimulation with the same chemical, the induction of an antigen-specific T cell response can be assessed by the detection of T cell proliferation, cytokine production or surface marker expression. The hTCPA still needs optimization but may nevertheless be a valuable in vitro assay within an integrated testing strategy aiming at replacement of animal testing for contact allergen identification.
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Esser, P.R., Martin, S.F. (2017). The Human T Cell Priming Assay (hTCPA). In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_31
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DOI: https://doi.org/10.1007/978-3-319-50353-0_31
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