Abstract
Immunohistochemical analysis of formalin-fixed, paraffin-embedded (FFPE) tissues can evaluate the characteristics of immune cells in the same tissue that is routinely collected for toxicologic pathology assessment. This chapter will address which antigen markers are best used for assessing toxicologic effects in the hematolymphoid system and for diagnosing hematolymphoid neoplasms in FFPE tissues from mice and rats. These markers can be normally present on immune cells, which may increase or decrease in number or undergo a change in their level of marker expression after exposure to a toxin, toxicant, or immunomodulator.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Adams B, Dorfler P, Aguzzi A, Kozmik Z, Urbanek P, Maurer-Fogy I, Busslinger M (1992) Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev 6:1589–1607
Agis H, Beil WJ, Bankl HC, Fureder W, Sperr WR, Ghannadan M, Baghestanian M, Sillaber C, Bettelheim P, Lechner K, Valent P (1996) Mast cell-lineage versus basophil lineage involvement in myeloproliferative and myelodysplastic syndromes: diagnostic role of cell-immunophenotyping. Leuk Lymphoma 22:187–204
Alison MR (1995) Assessing cellular proliferation: what’s worth measuring? Hum Exp Toxicol 14:935–944
Amanzada A, Malik IA, Nischwitz M, Sultan S, Naz N, Ramadori G (2011) Myeloperoxidase and elastase are only expressed by neutrophils in normal and in inflamed liver. Histochem Cell Biol 135:305–315
Arber DA, Strickler JG, Chen YY, Weiss LM (1997) Splenic vascular tumors: a histologic, immunophenotypic, and virologic study. Am J Surg Pathol 21:827–835
Arber DA, Tamayo R, Weiss LM (1998) Paraffin section detection of the c-kit gene product (CD117) in human tissues: value in the diagnosis of mast cell disorders. Hum Pathol 29:498–504
Arber DA, Weiss LM (1993) CD43: A Review. Appl Immunochistochem 1:88–96
Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K (2005) Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis. Proc Natl Acad Sci U S A 102:18105–18110
Asselin-Paturel C, Brizard G, Pin JJ, Briere F, Trinchieri G (2003) Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J Immunol 171:6466–6477
Batchelder CA, Duru N, Lee CI, Baker CA, Swainson L, Mccune JM, Tarantal AF (2014) Myeloid-lymphoid ontogeny in the rhesus monkey (Macaca mulatta). Anat Rec (Hoboken) 297:1392–1406
Bentolila LA, Fanton d’Andon M, Nguyen QT, Martinez O, Rougeon F, Doyen N (1995) The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization. EMBO J 14:4221–4229
Bhandoola A, Sambandam A (2006) From stem cell to T cell: one route or many? Nat Rev Immunol 6:117–126
Bhargava P, Kallakury BV, Ross JS, Azumi N, Bagg A (2007) CD79a is heterogeneously expressed in neoplastic and normal myeloid precursors and megakaryocytes in an antibody clone-dependent manner. Am J Clin Pathol 128:306–313
Biermann H, Pietz B, Dreier R, Schmid KW, Sorg C, Sunderkotter C (1999) Murine leukocytes with ring-shaped nuclei include granulocytes, monocytes, and their precursors. J Leukoc Biol 65:217–231
Birner P, Ritzi M, Musahl C, Knippers R, Gerdes J, Voigtlander T, Budka H, Hainfellner JA (2001) Immunohistochemical detection of cell growth fraction in formalin-fixed and paraffin-embedded murine tissue. Am J Pathol 158:1991–1996
Blasius AL, Barchet W, Cella M, Colonna M (2007) Development and function of murine B220+CD11c+NK1.1+ cells identify them as a subset of NK cells. J Exp Med 204:2561–2568
Bogue M, Gilfillan S, Benoist C, Mathis D (1992) Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny. Proc Natl Acad Sci U S A 89:11011–11015
Borowitz, M. J., and Chan, J. K. C. 2008. B lymphoblastic leukaemia/lymphoma, not otherwise specified. WHO classification of tumours of haematopoietic and lymphoid tissues.pp 168
Bouayad D, Pederzoli-Ribeil M, Mocek J, Candalh C, Arlet JB, Hermine O, Reuter N, Davezac N, Witko-Sarsat V (2012) Nuclear-to-cytoplasmic relocalization of the proliferating cell nuclear antigen (PCNA) during differentiation involves a chromosome region maintenance 1 (CRM1)-dependent export and is a prerequisite for PCNA antiapoptotic activity in mature neutrophils. J Biol Chem 287:33812–33825
Bowen MA, Lee RK, Miragliotta G, Nam SY, Podack ER (1996) Structure and expression of murine CD30 and its role in cytokine production. J Immunol 156:442–449
Bravo R, Macdonald-Bravo H (1987) Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. J Cell Biol 105:1549–1554
Brenner RM, Slayden OD, Rodgers WH, Critchley HO, Carroll R, Nie XJ, Mah K (2003) Immunocytochemical assessment of mitotic activity with an antibody to phosphorylated histone H3 in the macaque and human endometrium. Hum Reprod 18:1185–1193
Brown KE, Brunt EM, Heinecke JW (2001) Immunohistochemical detection of myeloperoxidase and its oxidation products in Kupffer cells of human liver. Am J Pathol 159:2081–2088
Burkhard MJ, Bienzle D (2013) Making sense of lymphoma diagnostics in small animal patients. Vet Clin North Am Small Anim Pract 43(1331-47):vii
Carbone A, Manconi R, Poletti A, Colombatti A, Tirelli U, Volpe R (1986) S-100 protein, fibronectin, and laminin immunostaining in lymphomas of follicular center cell origin. Cancer 58:2169–2176
Celis JE, Madsen P (1986) Increased nuclear cyclin/PCNA antigen staining of non S-phase transformed human amnion cells engaged in nucleotide excision DNA repair. FEBS Lett 209:277–283
Ceredig R, Rolink T (2002) A positive look at double-negative thymocytes. Nat Rev Immunol 2:888–897
Chen Z, Irani AA, Bradford TR, Craig SS, Newlands G, Miller H, Huff T, Simmons WH, Schwartz LB (1993) Localization of rat tryptase to a subset of the connective tissue type of mast cell. J Histochem Cytochem 41:961–969
Chiarle R, Podda A, Prolla G, Podack ER, Thorbecke GJ, Inghirami G (1999) CD30 overexpression enhances negative selection in the thymus and mediates programmed cell death via a Bcl-2-sensitive pathway. J Immunol 163:194–205
Chilosi M, Pizzolo G (1995) Review of terminal deoxynecleotidyl transferase. Appl Immunohistochem 3:209–221
Chilosi M, Pizzolo G, Caligaris-Cappio F, Ambrosetti A, Vinante F, Morittu L, Bonetti F, Fiore-Donati L, Janossy G (1985) Immunohistochemical demonstration of follicular dendritic cells in bone marrow involvement of B-cell chronic lymphocytic leukemia. Cancer 56:328–332
Chu P, Weiss L (2009) Modern immunohistochemistry. Cambridge University Press, Cambridge, UK
Chu P, Weiss L, Chang K, Arber D (1999) Practical applications of immunohistochemistry in hematolymphoid neoplasms. Ann Diagn Pathol 3:104–133
Chuang SS, Jung YC, Li CY (2000) von Willebrand factor is the most reliable immunohistochemical marker for megakaryocytes of myelodysplastic syndrome and chronic myeloproliferative disorders. Am J Clin Pathol 113:506–511
Coltrera MD, Gown AM (1991) PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines. J Histochem Cytochem 39:23–30
Corsetti MT, Calabi F (1997) Lineage- and stage-specific expression of runt box polypeptides in primitive and definitive hematopoiesis. Blood 89:2359–2368
Costain DJ, Guha AK, Liwski RS, Lee TD (2001) Murine hypodense eosinophils induce tumour cell apoptosis by a granzyme B-dependent mechanism. Cancer Immunol Immunother 50:293–299
Crawford JM, Barton RW (1986) Thy-1 glycoprotein: structure, distribution, and ontogeny. Lab Invest 54:122–135
Crocker PR, Jefferies WA, Clark SJ, Chung LP, Gordon S (1987) Species heterogeneity in macrophage expression of the CD4 antigen. J Exp Med 166:613–618
DAKO. 2007. Cross-reactivity study 3287 [Online]. Accessed 16 Feb 2017 from http://www.dako.com/us/cross-reactivity_study_3287.pdf
Davies M, Harris S, Rudland P, Barraclough R (1995) Expression of the rat, S-100-related, calcium-binding protein gene, p9Ka, in transgenic mice demonstrates different patterns of expression between these two species. DNA Cell Biol 14:825–832
Debili N, Issaad C, Masse JM, Guichard J, Katz A, Breton-Gorius J, Vainchenker W (1992) Expression of CD34 and platelet glycoproteins during human megakaryocytic differentiation. Blood 80:3022–3035
Di Primio R, Trubiani O, Bollum FJ (1992) Ultrastructural localization of Terminal deoxynucleotidyl Transferase (TdT) in rat thymocytes. Thymus 19:183–190
Dik WA, Pike-Overzet K, Weerkamp F, De Ridder D, De Haas EF, Baert MR, Van Der Spek P, Koster EE, Reinders MJ, Van Dongen JJ, Langerak AW, Staal FJ (2005) New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling. J Exp Med 201:1715–1723
Drew E, Merkens H, Chelliah S, Doyonnas R, Mcnagny KM (2002) CD34 is a specific marker of mature murine mast cells. Exp Hematol 30:1211–1218
Elagib KE, Racke FK, Mogass M, Khetawat R, Delehanty LL, Goldfarb AN (2003) RUNX1 and GATA-1 coexpression and cooperation in megakaryocytic differentiation. Blood 101:4333–4341
Enami Y, Kato H, Murakami M, Fujioka T, Aoki T, Niiya T, Murai N, Ohtsuka K, Kusano M (2001) Anti-transforming growth factor-beta1 antibody transiently enhances DNA synthesis during liver regeneration after partial hepatectomy in rats. J Hepatobiliary Pancreat Surg 8:250–258
Enerback L (1981) The gut mucosal mast cell. Monogr Allergy 17:222–232
Escribano L, Ocqueteau M, Almeida J, Orfao A, San Miguel JF (1998) Expression of the c-kit (CD117) molecule in normal and malignant hematopoiesis. Leuk Lymphoma 30:459–466
Ewing-Finchem T (2008) Effects of Fixation and Decalcification on Kappa and Lambda Staining by Immunohistochemistry. Histologic 41:1–4
Falini B, Fizzotti M, Pucciarini A, Bigerna B, Marafioti T, Gambacorta M, Pacini R, Alunni C, Natali-Tanci L, Ugolini B, Sebastiani C, Cattoretti G, Pileri S, Dalla-Favera R, Stein H (2000) A monoclonal antibody (MUM1p) detects expression of the MUM1/IRF4 protein in a subset of germinal center B cells, plasma cells, and activated T cells. Blood 95:2084–2092
Falini, B., Flenghi, L., Pileri, S., Gambacorta, M., Bigerna, B., Durkop, H., Eitelbach, F., Thiele, J., Pacini, R., CAVALIERE, A. & ET AL. 1993. PG-M1: a new monoclonal antibody directed against a fixative-resistant epitope on the macrophage-restricted form of the CD68 molecule. Am J Pathol, 142, 1359-1372.
Fedrowitz M, Westermann J, Loscher W (2002) Magnetic field exposure increases cell proliferation but does not affect melatonin levels in the mammary gland of female Sprague Dawley rats. Cancer Res 62:1356–1363
Fehniger TA, Cai SF, Cao X, Bredemeyer AJ, Presti RM, French AR, Ley TJ (2007) Acquisition of murine NK cell cytotoxicity requires the translation of a pre-existing pool of granzyme B and perforin mRNAs. Immunity 26:798–811
Flotte TJ, Springer TA, Thorbecke GJ (1983) Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets. Am J Pathol 111:112–124
Foley J, Ton T, Maronpot R, Butterworth B, Goldsworthy TL (1993) Comparison of proliferating cell nuclear antigen to tritiated thymidine as a marker of proliferating hepatocytes in rats. Environ Health Perspect 101(Suppl 5):199–205
Fong LY, Magee PN (1999) Dietary zinc deficiency enhances esophageal cell proliferation and N-nitrosomethylbenzylamine (NMBA)-induced esophageal tumor incidence in C57BL/6 mouse. Cancer Lett 143:63–69
Francke A, Herold J, Weinert S, Strasser RH, Braun-Dullaeus RC (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties. J Histochem Cytochem 59:813–825
Frigeri LG, Liu FT (1992) Surface expression of functional IgE binding protein, an endogenous lectin, on mast cells and macrophages. J Immunol 148:861–867
Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, Stein H (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol 133:1710–1715
Gerlach C, Golding M, Larue L, Alison MR, Gerdes J (1997) Ki-67 immunoexpression is a robust marker of proliferative cells in the rat. Lab Invest 77:697–698
Gewirtz AM (1995) Megakaryocytopoiesis: the state of the art. Thromb Haemost 74:204–209
Godfrey DI, Kennedy J, Suda T, Zlotnik A (1993) A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J Immunol 150:4244–4252
Godfrey DI, Zlotnik A, Suda T (1992) Phenotypic and functional characterization of c-kit expression during intrathymic T cell development. J Immunol 149:2281–2285
Goldsworthy TL, Morgan KT, Popp JA, Butterworth BE (1991) Guidelines for measuring chemically induced cell proliferation in specific rodent target organs. Prog Clin Biol Res 369:253–284
Gordon S, Lawson L, Rabinowitz S, Crocker PR, Morris L, Perry VH (1992) Antigen markers of macrophage differentiation in murine tissues. Curr Top Microbiol Immunol 181:1–37
Gordon S, Lee S, Morris L, Rabinowitz S (1986) Trophic and defense functions of murine macrophages. In: In Mechanism of Host Resistance to Infectious Agents, Agents, Tumors, and Allografts. Rockefeller University Press, New York, NY
Gordon S, Martinez FO (2010) Alternative activation of macrophages: mechanism and functions. Immunity 32:593–604
Gordon, S., Pluddemann, A. & Martinez Estrada, F. 2014. Macrophage heterogeneity in tissues: phenotypic diversity and functions. Immunol Rev, 262, 36-55.
Gouon-Evans V, Lin EY, Pollard JW (2002) Requirement of macrophages and eosinophils and their cytokines/chemokines for mammary gland development. Breast Cancer Res 4:155–164
Gratzner HG (1982) Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science 218:474–475
Grizzle WE (2009) Special symposium: fixation and tissue processing models. Biotech Histochem 84:185–193
Gualco G, Weiss LM, Bacchi CE (2010) MUM1/IRF4: A Review. Appl Immunohistochem Mol Morphol 18:301–310
Gurish MF, Friend DS, Webster M, Ghildyal N, Nicodemus CF, Stevens RL (1997) Mouse mast cells that possess segmented/multi-lobular nuclei. Blood 90:382–390
Hall PA, Levison DA, Woods AL, Yu CC, Kellock DB, Watkins JA, Barnes DM, Gillett CE, Camplejohn R, Dover R (1990) Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 162:285–294
Hallgren J, Gurish MF (2007) Pathways of murine mast cell development and trafficking: tracking the roots and routes of the mast cell. Immunol Rev 217:8–18
Han BS, Park CB, Takasuka N, Naito A, Sekine K, Nomura E, Taniguchi H, Tsuno T, Tsuda H (2001) A ferulic acid derivative, ethyl 3-(4′-geranyloxy-3-methoxyphenyl)-2-propenoate, as a new candidate chemopreventive agent for colon carcinogenesis in the rat. Jpn J Cancer Res 92:404–409
Haniffa M, Collin M, Ginhoux F (2013) Ontogeny and functional specialization of dendritic cells in human and mouse. Adv Immunol 120:1–49
Hans F, Dimitrov S (2001) Histone H3 phosphorylation and cell division. Oncogene 20:3021–3027
Hao, X., Fredrickson, T. N., Chattopadhyay, S. K., Han, W., Qi, C. F., Wang, Z., Ward, J. M., Hartley, J. W. & Morse, H. C., 3RD 2010. The histopathologic and molecular basis for the diagnosis of histiocytic sarcoma and histiocyte-associated lymphoma of mice. Vet Pathol, 47, 434-445.
Hao, X., Shin, M. S., Zhou, J. X., Lee, C. H., Qi, C. F., Naghashfar, Z., Hartley, J. W., Fredrickson, T. N., Ward, J. M. & Morse, H. C., 3RD 2006. Histologic and molecular characterizations of megakaryocytic leukemia in mice. Leuk Res, 30, 397-406.
Hard GC, Snowden RT (1991) Hyaline droplet accumulation in rodent kidney proximal tubules: an association with histiocytic sarcoma. Toxicol Pathol 19:88–97
Hardy RR, Kincade PW, Dorshkind K (2007) The protean nature of cells in the B lymphocyte lineage. Immunity 26:703–714
Hirsch S, Gordon S (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18:229–239
Hisa T, Spence SE, Rachel RA, Fujita M, Nakamura T, Ward JM, Devor-Henneman DE, Saiki Y, Kutsuna H, Tessarollo L, Jenkins NA, Copeland NG (2004) Hematopoietic, angiogenic and eye defects in Meis1 mutant animals. EMBO J 23:450–459
Horny H, Sotlar K, Valent P (2010) Differential diagnoses of systemic mastocytosis in routinely processed bone marrow biopsy specimens: a review. Pathobiology 77:169–180
Horny, H., Sotlar, K. & Valent, P. 2011. Mastocytosis. In: SAUNDERS, E. (ed.) Hematopathology. 1st edn. Elsevier Saunders
Hume DA (2008) Macrophages as APC and the dendritic cell myth. J Immunol 181:5829–5835
Hume DA, Robinson AP, Macpherson GG, Gordon S (1983) The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J Exp Med 158:1522–1536
Hung SI, Chang AC, Kato I, Chang NC (2002) Transient expression of Ym1, a heparin-binding lectin, during developmental hematopoiesis and inflammation. J Leukoc Biol 72:72–82
Imai Y, Ibata I, Ito D, Ohsawa K, Kohsaka S (1996) A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage. Biochem Biophys Res Commun 224:855–862
Ito T, Mitui H, Udaka N, Hayashi H, Okudela K, Kanisawa M, Kitamura H (1998) Ki-67 (MIB 5) immunostaining of mouse lung tumors induced by 4-nitroquinoline 1-oxide. Histochem Cell Biol 110:589–593
Jeong HK, Ji K, Min K, Joe EH (2013) Brain inflammation and microglia: facts and misconceptions. Exp Neurol 22:59–67
Kaiserling E, Xiao JC, Ruck P, Horny HP (1993) Aberrant expression of macrophage-associated antigens (CD68 and Ki-M1P) by Schwann cells in reactive and neoplastic neural tissue. Light- and electron-microscopic findings. Mod Pathol 6:463–468
Kalled SL, Siva N, Stein H, Reinherz EL (1995) The distribution of CD10 (NEP 24.11, CALLA) in humans and mice is similar in non-lymphoid organs but differs within the hematopoietic system: absence on murine T and B lymphoid progenitors. Eur J Immunol 25:677–687
Kamath AT, Pooley J, O’keeffe MA, Vremec D, Zhan Y, Lew AM, D’amico A, Wu L, Tough DF, Shortman K (2000) The development, maturation, and turnover rate of mouse spleen dendritic cell populations. J Immunol 165:6762–6770
Kamel OW, Warnke RA, Banks PM (1992) Localization of proliferating cell nuclear antigen (PCNA/Cyclin) in workshop cases of Hodgkin’s disease and non-Hodgkin’s lymphoma. Semin Diagn Pathol 9:311–314
Kap, Y. S., Van Meurs, M., Van Driel, N., Koopman, G., Melief, M. J., Brok, H. P., Laman, J. D. & T HART, B. A. 2009. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem, 57, 1159-1167.
Kaufman G, D’ovidio R, Kaldawy A, Assy B, Ullmann Y, Etzioni A, Paus R, Gilhar A (2010) An unexpected twist in alopecia areata pathogenesis: are NK cells protective and CD49b+ T cells pathogenic? Exp Dermatol 19:e347–e349
Kawamura T, Takeda K, Mendiratta SK, Kawamura H, Van Kaer L, Yagita H, Abo T, Okumura K (1998) Critical role of NK1+ T cells in IL-12-induced immune responses in vivo. J Immunol 160:16–19
Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, Mclaurin J, Williams K (2006) CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol 168:822–834
Kina T, Ikuta K, Takayama E, Wada K, Majumdar AS, Weissman IL, Katsura Y (2000) The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280–287
King BE, Chen C, Locker J, Kant J, Okuyama K, Falini B, Swerdlow SH (2000) Immunophenotypic and genotypic markers of follicular center cell neoplasia in diffuse large B-cell lymphomas. Mod Pathol 13:1219–1231
Klockars M, Azar HA, Hermida R, Isobe T, Hsu CC, Ansari H, Osserman EF (1974) The relationship of lysozyme to the nephropathy in chloroleukemic rats and the effects of lysozyme loading on normal rat kidneys. Cancer Res 34:47–60
Klockars M, Osserman EF (1974) Localization of lysozyme in normal rat tissues by an immunoperoxidase method. J Histochem Cytochem 22:139–146
Kohler C (2007) Allograft inflammatory factor-1/Ionized calcium-binding adapter molecule 1 is specifically expressed by most subpopulations of macrophages and spermatids in testis. Cell Tissue Res 330:291–302
Kremmidiotis G, Zola H (1995) Changes in CD44 expression during B cell differentiation in the human tonsil. Cell Immunol 161:147–157
Kronenberg M, Gapin L (2002) The unconventional lifestyle of NKT cells. Nat Rev Immunol 2:557–568
Kunder S, Calzada-Wack J, Holzlwimmer G, Muller J, Kloss C, Howat W, Schmidt J, Hofler H, Warren M, Quintanilla-Martinez L (2007) A comprehensive antibody panel for immunohistochemical analysis of formalin-fixed, paraffin-embedded hematopoietic neoplasms of mice: analysis of mouse specific and human antibodies cross-reactive with murine tissue. Toxicol Pathol 35:366–375
Kurki P, Vanderlaan M, Dolbeare F, Gray J, Tan EM (1986) Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle. Exp Cell Res 166:209–219
Lagasse E, Weissman IL (1996) Flow cytometric identification of murine neutrophils and monocytes. J Immunol Methods 197:139–150
Laloux V, Beaudoin L, Ronet C, Lehuen A (2002) Phenotypic and functional differences between NKT cells colonizing splanchnic and peripheral lymph nodes. J Immunol 168:3251–3258
Lappin PB, Black LE (2003) Immune modulator studies in primates: the utility of flow cytometry and immunohistochemistry in the identification and characterization of immunotoxicity. Toxicol Pathol 31(Suppl):111–118
Lau SK, Chu PG, Weiss LM (2004) CD163: a specific marker of macrophages in paraffin-embedded tissue samples. Am J Clin Pathol 122:794–801
Lee JJ, Mcgarry MP (2007) When is a mouse basophil not a basophil? Blood 109:859–861
Leenen PJ, De Bruijn MF, Voerman JS, Campbell PA, Van Ewijk W (1994) Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods 174:5–19
Leenen PJ, Radosevic K, Voerman JS, Salomon B, Van Rooijen N, Klatzmann D, Van Ewijk W (1998) Heterogeneity of mouse spleen dendritic cells: in vivo phagocytic activity, expression of macrophage markers, and subpopulation turnover. J Immunol 160:2166–2173
Lesley J, Schulte R, Trotter J, Hyman R (1988) Qualitative and quantitative heterogeneity in Pgp-1 expression among murine thymocytes. Cell Immunol 112:40–54
Li C-Y (2007) Mastocytosis. In: Hsi ED (ed) Hematopathology. Elsevier Saunders, Philadelphia, PA
Li SL, Kaaya E, Feichtinger H, Biberfeld G, Biberfeld P (1993) Immunohistochemical distribution of leucocyte antigens in lymphoid tissues of cynomolgus monkeys (Macaca fascicularis). J Med Primatol 22:285–293
Li WV, Kapadia SB, Sonmez-Alpan E, Swerdlow SH (1996) Immunohistochemical characterization of mast cell disease in paraffin sections using tryptase, CD68, myeloperoxidase, lysozyme, and CD20 antibodies. Mod Pathol 9:982–988
Lopez AF, Strath M, Sanderson CJ (1984) Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies. Br J Haematol 57:489–494
Lynch F, Ceredig R (1989) Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation. Eur J Immunol 19:223–229
Malorny U, Michels E, Sorg C (1986) A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res 243:421–428
Marchesi F, Minucci S, Pelicci PG, Gobbi A, Scanziani E (2006) Immunohistochemical detection of Ym1/Ym2 chitinase-like lectins associated with hyalinosis and polypoid adenomas of the transitional epithelium in a mouse with acute myeloid leukemia. Vet Pathol 43:773–776
Matsui Y, Natori S, Obinata M (1985) Induction of glycophorin gene expression in cultured murine erythroleukemia cells. Differentiation 29:268–274
Mazzucchelli R, Durum SK (2007) Interleukin-7 receptor expression: intelligent design. Nat Rev Immunol 7:144–154
Mccafferty DM, Miampamba M, Sihota E, Sharkey KA, Kubes P (1999) Role of inducible nitric oxide synthase in trinitrobenzene sulphonic acid induced colitis in mice. Gut 45:864–873
Mccomb RD, Jones TR, Pizzo SV, Bigner DD (1982) Specificity and sensitivity of immunohistochemical detection of factor VIII/von Willebrand factor antigen in formalin-fixed paraffin-embedded tissue. J Histochem Cytochem 30:371–377
Mcdorman KC, Rojko J, Satterwhite C, Morrison J (2013) Special techniques in toxicologic pathology. In: Haschek-Hock WMR, Wallig MA, Rousseaux CG (eds) Haschek and Rousseaux’s Handbook of toxicologic pathology, 3rd edn. Elsevier, Amsterdam
Mcgarry MP, Stewart CC (1991) Murine eosinophil granulocytes bind the murine macrophage-monocyte specific monoclonal antibody F4/80. J Leukoc Biol 50:471–478
Mckenna K, Beignon AS, Bhardwaj N (2005) Plasmacytoid dendritic cells: linking innate and adaptive immunity. J Virol 79:17–27
Mikaelian I, Nanney LB, Parman KS, Kusewitt DF, Ward JM, Naf D, Krupke DM, Eppig JT, Bult CJ, Seymour R, Ichiki T, Sundberg JP (2004) Antibodies that label paraffin-embedded mouse tissues: a collaborative endeavor. Toxicol Pathol 32:181–191
Milner JD, Orekov T, Ward JM, Cheng L, Torres-Velez F, Junttila I, Sun G, Buller M, Morris SC, Finkelman FD, Paul WE (2010) Sustained IL-4 exposure leads to a novel pathway for hemophagocytosis, inflammation, and tissue macrophage accumulation. Blood 116:2476–2483
Miyachi K, Fritzler MJ, Tan EM (1978) Autoantibody to a nuclear antigen in proliferating cells. J Immunol 121:2228–2234
Morris GF, Mathews MB (1989) Regulation of proliferating cell nuclear antigen during the cell cycle. J Biol Chem 264:13856–13864
Morse, H. C., 3RD, Anver, M. R., Fredrickson, T. N., Haines, D. C., Harris, A. W., Harris, N. L., Jaffe, E. S., Kogan, S. C., Maclennan, I. C., Pattengale, P. K., Ward, J. M. (2002). Bethesda proposals for classification of lymphoid neoplasms in mice. Blood, 100, 246-258.
Morton J, Coles B, Wright K, Gallimore A, Morrow JD, Terry ES, Anning PB, Morgan BP, Dioszeghy V, Kuhn H, Chaitidis P, Hobbs AJ, Jones SA, O’donnell VB (2008) Circulating neutrophils maintain physiological blood pressure by suppressing bacteria and IFNgamma-dependent iNOS expression in the vasculature of healthy mice. Blood 111:5187–5194
Muler JH, Valdez R, Hayes C, Kaminski MS (2002) Acute megakaryocytic leukemia presenting as hypercalcemia with skeletal lytic lesions. Eur J Haematol 68:392–396
Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB (2003) Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem 51:1681–1688
Nabe T, Matsuya K, Akamizu K, Fujita M, Nakagawa T, Shioe M, Kida H, Takiguchi A, Wakamori H, Fujii M, Ishihara K, Akiba S, Mizutani N, Yoshino S, Chaplin DD (2013) Roles of basophils and mast cells infiltrating the lung by multiple antigen challenges in asthmatic responses of mice. Br J Pharmacol 169:462–476
Nagendra S, Schlueter AJ (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58:195–200
Nagra RM, Becher B, Tourtellotte WW, Antel JP, Gold D, Paladino T, Smith RA, Nelson JR, Reynolds WF (1997) Immunohistochemical and genetic evidence of myeloperoxidase involvement in multiple sclerosis. J Neuroimmunol 78:97–107
Nair MG, Cochrane DW, Allen JE (2003) Macrophages in chronic type 2 inflammation have a novel phenotype characterized by the abundant expression of Ym1 and Fizz1 that can be partly replicated in vitro. Immunol Lett 85:173–180
NCI. 2016. PHL Murine Immunohistochemistry Database [Online]. National Cancer Institute (NCI)-Frederick. Accessed 16 Feb 2016 from https://ncifrederick.cancer.gov/rtp/lasp/phl/immuno/
Neuhauser TS, Derringer GA, Thompson LD, Fanburg-Smith JC, Miettinen M, Saaristo A, Abbondanzo SL (2000) Splenic angiosarcoma: a clinicopathologic and immunophenotypic study of 28 cases. Mod Pathol 13:978–987
Newman RA, Boss MA (1980) Expression of binding sites for peanut agglutinin during murine B lymphocyte differentiation. Immunology 40:193–200
NIEHS. 2016. Immunohistochemistry [Online] Available: https://www.niehs.nih.gov/research/resources/protocols/protocols-immuno/index.cfm [Accessed February 16, 2016].
Nio J, Fujimoto W, Konno A, Kon Y, Owhashi M, Iwanaga T (2004) Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry. Histochem Cell Biol 121:473–482
O’connell FP, Pinkus JL, Pinkus GS (2004) CD138 (syndecan-1), a plasma cell marker immunohistochemical profile in hematopoietic and nonhematopoietic neoplasms. Am J Clin Pathol 121:254–263
Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H (2007) Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood 110:913–920
Orazi A, Cotton J, Cattoretti G, Kotylo PK, John K, Manning JT, Neiman RS (1994) Terminal deoxynucleotidyl transferase staining in acute leukemia and normal bone marrow in routinely processed paraffin sections. Am J Clin Pathol 102:640–645
Ormerod LD, Osborn KG, Lowenstine LJ, Meyer PR, Parker JW, Smith RE, Taylor CR (1988) Lymphoid immunohistochemistry of macaque primates. Clin Exp Immunol 74:435–442
Orsmark C, Skoog T, Jeskanen L, Kere J, Saarialho-Kere U (2007) Expression of allograft inflammatory factor-1 in inflammatory skin disorders. Acta Derm Venereol 87:223–227
Osmond DG, Rolink A, Melchers F (1998) Murine B lymphopoiesis: towards a unified model. Immunol Today 19:65–68
Pardo J, Wallich R, Ebnet K, Iden S, Zentgraf H, Martin P, Ekiciler A, Prins A, Mullbacher A, Huber M, Simon MM (2007) Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin. Cell Death Differ 14:1768–1779
Patel JL, Smith LM, Anderson J, Abromowitch M, Campana D, Jacobsen J, Lones MA, Gross TG, Cairo MS, Perkins SL (2012) The immunophenotype of T-lymphoblastic lymphoma in children and adolescents: a Children’s Oncology Group report. Br J Haematol 159:454–461
Pedrocchi M, Hauer CR, Schafer BW, Erne P, Heizmann CW (1993) Analysis of CA(2+)-binding S100 proteins in human heart by HPLC-electrospray mass spectrometry. Biochem Biophys Res Commun 197:529–535
Perry VH, Crocker PR, Gordon S (1992) The blood-brain barrier regulates the expression of a macrophage sialic acid-binding receptor on microglia. J Cell Sci 101(Pt 1):201–207
Perry VH, Lawson LJ, Reid DM (1994) Biology of the mononuclear phagocyte system of the central nervous system and HIV infection. J Leukoc Biol 56:399–406
Pileri SA, Grogan TM, Harris NL, Banks P, Campo E, Chan JK, Favera RD, Delsol G, De Wolf-Peeters C, Falini B, Gascoyne RD, Gaulard P, Gatter KC, Isaacson PG, Jaffe ES, Kluin P, Knowles DM, Mason DY, Mori S, Muller-Hermelink HK, Piris MA, Ralfkiaer E, Stein H, Su IJ, Warnke RA, Weiss LM (2002) Tumours of histiocytes and accessory dendritic cells: an immunohistochemical approach to classification from the International Lymphoma Study Group based on 61 cases. Histopathology 41:1–29
Pilozzi E, Pulford K, Jones M, Muller-Hermelink HK, Falini B, Ralfkiaer E, Pileri S, Pezzella F, De Wolf-Peeters C, Arber D, Stein H, Mason D, Gatter K (1998) Co-expression of CD79a (JCB117) and CD3 by lymphoblastic lymphoma. J Pathol 186:140–143
Pinkus GS, Lones MA, Matsumura F, Yamashiro S, Said JW, Pinkus JL (2002) Langerhans cell histiocytosis immunohistochemical expression of fascin, a dendritic cell marker. Am J Clin Pathol 118:335–343
Pinkus GS, Pinkus JL (1991) Myeloperoxidase: a specific marker for myeloid cells in paraffin sections. Mod Pathol 4:733–741
Piriou-Guzylack L, Salmon H (2008) Membrane markers of the immune cells in swine: an update. Vet Res 39:54
Pusztaszeri MP, Seelentag W, Bosman FT (2006) Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues. J Histochem Cytochem 54:385–395
Raffegerst SH, Hoelzlwimmer G, Kunder S, Mysliwietz J, Quintanilla-Martinez L, Schendel DJ (2009) Diverse hematological malignancies including hodgkin-like lymphomas develop in chimeric MHC class II transgenic mice. PLoS One 4:e8539
Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426
Ramos-Vara JA, Miller MA (2014) When tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemistry--the red, brown, and blue technique. Vet Pathol 51:42–87
Rausch PG, Moore TG (1975) Granule enzymes of polymorphonuclear neutrophils: A phylogenetic comparison. Blood 46:913–919
Ree HJ, Hsu SM (1983) Lectin histochemistry of malignant tumors. I. Peanut agglutinin (PNA) receptors in follicular lymphoma and follicular hyperplasia: an immunohistochemical study. Cancer 51:1631–1638
Rehg JE, Bush D, Ward JM (2012) The utility of immunohistochemistry for the identification of hematopoietic and lymphoid cells in normal tissues and interpretation of proliferative and inflammatory lesions of mice and rats. Toxicol Pathol 40:345–374
Rehg JE, Rahija R, Bush D, Bradley A, Ward JM (2015) Immunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice. [Corrected]. Toxicol Pathol 43:1025–1034
Rehg JE, Sundberg JP (2008) Utility of antiPax5 in the diagnosis of lymphoproliferative disorders and neoplasia in mice. Comp Med 58:246–252
Rensen SS, Slaats Y, Nijhuis J, Jans A, Bieghs V, Driessen A, Malle E, Greve JW, Buurman WA (2009) Increased hepatic myeloperoxidase activity in obese subjects with nonalcoholic steatohepatitis. Am J Pathol 175:1473–1482
Richter N, Petersson AS, Wennmalm A, Schimke I (1999) Detection of an approximately 100 kD protein with strong immunoreactivity to antibodies specific for inducible nitric oxide synthase (iNOS) but without NOS activity in neutrophils of patients suffering from sepsis: results of a preliminary study. Inflamm Res 48:651–656
Rosas M, Thomas B, Stacey M, Gordon S, Taylor PR (2010) The myeloid 7/4-antigen defines recently generated inflammatory macrophages and is synonymous with Ly-6B. J Leukoc Biol 88:169–180
Rose ML, Malchiodi F (1981) Binding of peanut lectin to thymic cortex and germinal centres of lymphoid tissue. Immunology 42:583–591
Sadahira Y, Kanzaki A, Wada H, Yawata Y (1999) Immunohistochemical identification of erythroid precursors in paraffin embedded bone marrow sections: spectrin is a superior marker to glycophorin. J Clin Pathol 52:919–921
Schafernak KT, Variakojis D, Goolsby CL, Tucker RM, Martinez-Escala ME, Smith FA, Dittman D, Chenn A, Guitart J (2014) Clonality assessment of cutaneous B-cell lymphoid proliferations: a comparison of flow cytometry immunophenotyping, molecular studies, and immunohistochemistry/in situ hybridization and review of the literature. Am J Dermatopathol 36:781–795
Schlitzer A, Ginhoux F (2014) Organization of the mouse and human DC network. Curr Opin Immunol 26:90–99
Schluter C, Duchrow M, Wohlenberg C, Becker MH, Key G, Flad HD, Gerdes J (1993) The cell proliferation-associated antigen of antibody Ki-67: a very large, ubiquitous nuclear protein with numerous repeated elements, representing a new kind of cell cycle-maintaining proteins. J Cell Biol 123:513–522
Scholzen T, Gerdes J (2000) The Ki-67 protein: from the known and the unknown. J Cell Physiol 182:311–322
Schwarting R (1993) Little missed markers and Ki-67. Lab Invest 68:597–599
Schwarting R, Gerdes J, Durkop H, Falini B, Pileri S, Stein H (1989) BER-H2: a new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood 74:1678–1689
Scudamore CH, Kh HEATHER, Patterson LEE, Macdonald ANNA, Brown FIONA, Smith KEN (2011) The effect of post-mortem delay on immunohistochemical labelling--a short review. Comp Clin Pathol 20:95–101
Seagal J, Leider N, Wildbaum G, Karin N, Melamed D (2003) Increased plasma cell frequency and accumulation of abnormal syndecan-1plus T-cells in Igmu-deficient/lpr mice. Int Immunol 15:1045–1052
Sharon N (1983) Lectin receptors as lymphocyte surface markers. Adv Immunol 34:213–298
Shi SR, Shi Y, Taylor CR (2011) Antigen retrieval immunohistochemistry: review and future prospects in research and diagnosis over two decades. J Histochem Cytochem 59:13–32
Shivji KK, Kenny MK, Wood RD (1992) Proliferating cell nuclear antigen is required for DNA excision repair. Cell 69:367–374
Shortman K, Liu YJ (2002) Mouse and human dendritic cell subtypes. Nat Rev Immunol 2:151–161
Speece AJ (1964) Histochemical Distribution of Lysozyme Activity in Organs of Normal Mice and Radiation Chimeras. J Histochem Cytochem 12:384–391
Stewart CA, Walzer T, Robbins SH, Malissen B, Vivier E, Prinz I (2007) Germ-line and rearranged Tcrd transcription distinguish bona fide NK cells and NK-like gammadelta T cells. Eur J Immunol 37:1442–1452
Swerdlow SCE, Harris N, Jaffe E, Pileri S, Stein H, Thiele J, Vardiman J (2008) WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, Washington, DC, International Agency for Research on. World Health Organization, Cancer
Sykora KW, Tomeczkowski J, Reiter A (1997) C-kit receptors in childhood malignant lymphoblastic cells. Leuk Lymphoma 25:201–216
Tacchini-Cottier F, Zweifel C, Belkaid Y, Mukankundiye C, Vasei M, Launois P, Milon G, Louis JA (2000) An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major. J Immunol 165:2628–2636
Takahashi K, Isobe T, Ohtsuki Y, Sonobe H, Takeda I, Akagi T (1984) Immunohistochemical localization and distribution of S-100 proteins in the human lymphoreticular system. Am J Pathol 116:497–503
Takeda K, Hayakawa Y, Van Kaer L, Matsuda H, Yagita H, Okumura K (2000) Critical contribution of liver natural killer T cells to a murine model of hepatitis. Proc Natl Acad Sci U S A 97:5498–5503
Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S (2005) Macrophage receptors and immune recognition. Annu Rev Immunol 23:901–944
Thai TH, Kearney JF (2004) Distinct and opposite activities of human terminal deoxynucleotidyltransferase splice variants. J Immunol 173:4009–4019
Thomas, J., Haseman, J. K., Goodman, J. I., Ward, J. M., Loughran, T. P., JR. & SPENCER, P. J. 2007. A review of large granular lymphocytic leukemia in Fischer 344 rats as an initial step toward evaluating the implication of the endpoint to human cancer risk assessment. Toxicol Sci, 99, 3-19.
Torchia, E. C., Boyd, K., Rehg, J. E., QU, C. & BAKER, S. J. 2007. EWS/FLI-1 induces rapid onset of myeloid/erythroid leukemia in mice. Mol Cell Biol, 27, 7918-7934.
Toschi L, Bravo R (1988) Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis. J Cell Biol 107:1623–1628
Tripp CH, Chang-Rodriguez S, Stoitzner P, Holzmann S, Stossel H, Douillard P, Saeland S, Koch F, Elbe-Burger A, Romani N (2004) Ontogeny of Langerin/CD207 expression in the epidermis of mice. J Invest Dermatol 122:670–672
Tsuta K, Liu DC, Kalhor N, Wistuba II, Moran CA (2011) Using the mitosis-specific marker anti-phosphohistone H3 to assess mitosis in pulmonary neuroendocrine carcinomas. Am J Clin Pathol 136:252–259
Tung JW, Mrazek MD, Yang Y, Herzenberg LA, Herzenberg LA (2006) Phenotypically distinct B cell development pathways map to the three B cell lineages in the mouse. Proc Natl Acad Sci U S A 103:6293–6298
Ugajin T, Kojima T, Mukai K, Obata K, Kawano Y, Minegishi Y, Eishi Y, Yokozeki H, Karasuyama H (2009) Basophils preferentially express mouse Mast Cell Protease 11 among the mast cell tryptase family in contrast to mast cells. J Leukoc Biol 86:1417–1425
Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattei MG, Ait-Yahia S, Bates EE, Malissen B, Koch F, Fossiez F, Romani N, Lebecque S, Saeland S (2002) Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol 168:782–792
Van Den Berg TK, Puklavec MJ, Barclay AN, Dijkstra CD (2001) Monoclonal antibodies against rat leukocyte surface antigens. Immunol Rev 184:109–116
Voehringer D (2013) Protective and pathological roles of mast cells and basophils. Nat Rev Immunol 13:362–375
Vosshenrich CA, Lesjean-Pottier S, Hasan M, Richard Le-Goff O, Corcuff E, Mandelboim O, Di Santo JP (2007) CD11cloB220+ interferon-producing killer dendritic cells are activated natural killer cells. J Exp Med 204:2569–2578
Ward JM, Erexson CR, Faucette LJ, Foley JF, Dijkstra C, Cattoretti G (2006) Immunohistochemical markers for the rodent immune system. Toxicol Pathol 34:616–630
Ward JM, Rehg JE (2014) Rodent immunohistochemistry: pitfalls and troubleshooting. Vet Pathol 51:88–101
Ward JM, Rehg JE, Morse HC, 3RD (2012) Differentiation of rodent immune and hematopoietic system reactive lesions from neoplasias. Toxicol Pathol 40:425–434
Ward JM, Rehm S (1990) Applications of immunohistochemistry in rodent tumor pathology. Exp Pathol 40:301–312
Ward JM, Sheldon W (1993) Expression of mononuclear phagocyte antigens in histiocytic sarcoma of mice. Vet Pathol 30:560–565
Ward JM, Yoon M, Anver MR, Haines DC, Kudo G, Gonzalez FJ, Kimura S (2001) Hyalinosis and Ym1/Ym2 gene expression in the stomach and respiratory tract of 129S4/SvJae and wild-type and CYP1A2-null B6, 129 mice. Am J Pathol 158:323–332
Webster JD, Miller MA, Dusold D, Ramos-Vara J (2009) Effects of prolonged formalin fixation on diagnostic immunohistochemistry in domestic animals. J Histochem Cytochem 57:753–761
Weerkamp F, Pike-Overzet K, Staal FJ (2006) T-sing progenitors to commit. Trends Immunol 27:125–131
Weiss L, Arber D, Chang K (1994) CD68: A Review. Appl Immunohistochem 2:2–8
Weiss LM, Arber DA, Chang KL (1993) CD45: A Review. Appl Immunohistochem 1:166–181
Welle M (1997) Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase. J Leukoc Biol 61:233–245
Wick M (2006) Immunohistology of melanocytic neoplasm, Diagnostic immunohistochemistry. Churchill Livingstone, Elsevier, Philadelphia, PA
Woloschak GE, Krco CJ (1987) Regulation of kappa/lambda immunoglobulin light chain expression in normal murine lymphocytes. Mol Immunol 24:751–757
Wood RD, Shivji MK (1997) Which DNA polymerases are used for DNA-repair in eukaryotes? Carcinogenesis 18:605–610
Yamagishi H, Mochizuki Y, Hamakubo T, Obata K, Ugajin T, Sato S, Kawano Y, Minegishi Y, Karasuyama H (2011) Basophil-derived mouse mast cell protease 11 induces microvascular leakage and tissue edema in a mast cell-independent manner. Biochem Biophys Res Commun 415:709–713
Zhang, F. R., Tao, L. H., Shen, Z. Y., LV, Z., XU, L. Y. & LI, E. M. 2008. Fascin expression in human embryonic, fetal, and normal adult tissue. J Histochem Cytochem, 56, 193-199.
Zhu JD (1999) Myeloid cell-lineage and premylocytic-stage-specific- expression of the mouse myeloperoxidase gene is controlled at initiation as well as elongation levels of transcription. Cell Res 9:107–134
Zon LI, Gurish MF, Stevens RL, Mather C, Reynolds DS, Austen KF, Orkin SH (1991) GATA-binding transcription factors in mast cells regulate the promoter of the mast cell carboxypeptidase A gene. J Biol Chem 266:22948–22953
Acknowledgments
The authors thank Dorothy Bush for her excellent immunohistochemistry services, Debbie Cuffee for her secretarial assistance, and Keith Laycock for his editorial assistance.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this chapter
Cite this chapter
Rehg, J.E., Ward, J.M. (2017). Application of Immunohistochemistry in Toxicologic Pathology of the Hematolymphoid System. In: Parker, G. (eds) Immunopathology in Toxicology and Drug Development. Molecular and Integrative Toxicology. Humana Press, Cham. https://doi.org/10.1007/978-3-319-47377-2_10
Download citation
DOI: https://doi.org/10.1007/978-3-319-47377-2_10
Published:
Publisher Name: Humana Press, Cham
Print ISBN: 978-3-319-47375-8
Online ISBN: 978-3-319-47377-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)