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HPV Testing of Head and Neck Cancer in Clinical Practice

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HPV Infection in Head and Neck Cancer

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 206))

Abstract

The pathology laboratory has a central role in providing human papillomavirus (HPV) tests for patients with head and neck cancer. There is an extensive literature around HPV testing and a large number of proprietary HPV tests, which makes the field difficult to navigate. This review provides a concise contemporary overview of the evidence around HPV testing in head and neck cancer and signposts key publications, guideline documents and the most commonly used methods in clinical practice.

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References

  • Ang KK, Harris J, Wheeler R, Weber R, Rosenthal DI, Nguyen-Tan PF et al (2010) Human papillomavirus and survival of patients with oropharyngeal cancer. N Engl J Med 363(1):24–35

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bhatia A, Burtness B (2015) Human papillomavirus-associated oropharyngeal cancer: defining risk groups and clinical trials. J Clin Oncol 33(29):3243–3250

    Article  CAS  PubMed  Google Scholar 

  • Chung CH, Zhang Q, Kong CS, Harris J, Fertig EJ, Harari PM et al (2014) p16 protein expression and human papillomavirus status as prognostic biomarkers of nonoropharyngeal head and neck squamous cell carcinoma. J Clin Oncol 32(35):3930–3938

    Article  PubMed  PubMed Central  Google Scholar 

  • College of American Pathologists (2012) Protocol for the examination of specimens from patients with carcinomas of the pharynx. College of American Pathologists, USA

    Google Scholar 

  • Huang SH, Perez-Ordonez B, Liu FF, Waldron J, Ringash J, Irish J et al (2012) Atypical clinical behavior of p16-confirmed HPV-related oropharyngeal squamous cell carcinoma treated with radical radiotherapy. Int J Radiat Oncol Biol Phys 82(1):276–283

    Article  PubMed  Google Scholar 

  • ISO15189:2012. http://www.iso.org/iso/catalogue_detail?csnumber=56115 (Accessed 06.03.2016)

  • Jordan RC, Lingen MW, Perez-Ordonez B, He X, Pickard R, Koluder M et al (2012) Validation of methods for oropharyngeal cancer HPV status determination in US cooperative group trials. Am J Surg Pathol 36(7):945–954

    Article  PubMed  Google Scholar 

  • Leemans CR, Braakhuis BJ, Brakenhoff RH (2011) The molecular biology of head and neck cancer. Nat Rev Cancer 11(1):9–22

    Article  CAS  PubMed  Google Scholar 

  • Lewis JS Jr (2012) p16 Immunohistochemistry as a standalone test for risk stratification in oropharyngeal squamous cell carcinoma. Head Neck Pathol 6(Suppl 1):S75–S82

    Article  PubMed  Google Scholar 

  • Lewis JS Jr, Thorstad WL, Chernock RD, Haughey BH, Yip JH, Zhang Q et al (2010) p16 positive oropharyngeal squamous cell carcinoma: an entity with a favorable prognosis regardless of tumor HPV status. Am J Surg Pathol 34(8):1088–1096

    Article  PubMed  Google Scholar 

  • Maxwell JH, Kumar B, Feng FY, McHugh JB, Cordell KG, Eisbruch A et al (2010) HPV-positive/p16-positive/EBV-negative nasopharyngeal carcinoma in white North Americans. Head Neck 32(5):562–567

    PubMed  PubMed Central  Google Scholar 

  • McCord C, Xu J, Xu W, Qiu X, McComb RJ, Perez-Ordonez B et al (2013) Association of high-risk human papillomavirus infection with oral epithelial dysplasia. Oral Surg Oral Med Oral Pathol Oral Radiol 115(4):541–549

    Article  PubMed  Google Scholar 

  • Mehanna H, Franklin N, Compton N, Robinson M, Powell N, Biswas-Baldwin N et al (2016) Regional and geographic variability in human papillomavirus-associated oropharyngeal cancer: individual patient data and samples from four multi-national randomised trials. Head Neck. doi:10.1002/hed.24336

    PubMed Central  Google Scholar 

  • Mirghani H, Casiraghi O, Amen F, He M, Ma XJ, Saulnier P et al (2015) Diagnosis of HPV-driven head and neck cancer with a single test in routine clinical practice. Mod Pathol 28(12):1518–1527

    Article  CAS  PubMed  Google Scholar 

  • National Cancer Comprehensive Network (2014) Clinical practice guidelines in oncology: head and neck cancers. National Cancer Comprehensive Network, USA

    Google Scholar 

  • Perrone F, Gloghini A, Cortelazzi B, Bossi P, Licitra L, Pilotti S (2011) Isolating p16-positive/HPV-negative oropharyngeal cancer: an effort worth making. Am J Surg Pathol 35(5):774–777

    Article  PubMed  Google Scholar 

  • Rietbergen MM, Leemans CR, Bloemena E, Heideman DA, Braakhuis BJ, Hesselink AT, Witte BI, Baatenburg de Jong RJ, Meijer CJ, Snijders PJ, Brakenhoff RH (2013a) Increasing prevalence rates of HPV attributable oropharyngeal squamous cell carcinomas in the Netherlands as assessed by a validated test algorithm. Int J Cancer 132(7):1565–1571

    Google Scholar 

  • Rietbergen MM, Brakenhoff RH, Bloemena E, Witte BI, Snijders PJ, Heideman DA, et al (2013b) Human papillomavirus detection and comorbidity: critical issues in selection of patients with oropharyngeal cancer for treatment De-escalation trials. Ann Oncol 24(11):2740–2745

    Google Scholar 

  • Rietbergen MM, Witte BI, Velazquez ER, Snijders PJ, Bloemena E, Speel EJ, Brakenhoff RH, Kremer B, Lambin P, Leemans CR (2015) Different prognostic models for different patient populations: validation of a new prognostic model for patients with oropharyngeal cancer in Western Europe. Br J Cancer 112(11):1733–1736

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Robinson M, Sloan P, Shaw R (2010) Refining the diagnosis of oropharyngeal cancer using human papillomavirus testing. Oral Oncol 46(7):492–496

    Article  PubMed  Google Scholar 

  • Robinson M, Schache A, Sloan P, Thavaraj S (2012) HPV specific testing: a requirement for oropharyngeal squamous cell carcinoma patients. Head Neck Pathol. 6(Suppl 1):S83–S90

    Article  PubMed  Google Scholar 

  • Robinson M, Suh YE, Paleri V, Devlin D, Ayaz B, Pertl L et al (2013) Oncogenic human papillomavirus-associated nasopharyngeal carcinoma: an observational study of correlation with ethnicity, histological subtype and outcome in a UK population. Infect Agent Cancer. 8(1):30

    Article  PubMed  PubMed Central  Google Scholar 

  • Royal College of Pathologists (2013) Dataset for the reporting of mucosal malignancies of the pharynx. Royal College of Pathologists, UK

    Google Scholar 

  • Salazar CR, Anayannis N, Smith RV, Wang Y, Haigentz M Jr, Garg M et al (2014) Combined P16 and human papillomavirus testing predicts head and neck cancer survival. Int J Cancer 135(10):2404–2412

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schache AG, Liloglou T, Risk JM, Filia A, Jones TM, Sheard J et al (2011) Evaluation of human papilloma virus diagnostic testing in oropharyngeal squamous cell carcinoma: sensitivity, specificity, and prognostic discrimination. Clin Cancer Res 17(19):6262–6271

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schache AG, Liloglou T, Risk JM, Jones TM, Ma XJ, Wang H et al (2013) Validation of a novel diagnostic standard in HPV-positive oropharyngeal squamous cell carcinoma. Br J Cancer 108(6):1332–1339

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schache A, Croud J, Robinson M, Thavaraj S (2014) Human papillomavirus testing in head and neck squamous cell carcinoma: best practice for diagnosis. Methods Mol Biol 1180:237–255

    Article  PubMed  Google Scholar 

  • Singhi AD, Westra WH (2010) Comparison of human papillomavirus in situ hybridization and p16 immunohistochemistry in the detection of human papillomavirus-associated head and neck cancer based on a prospective clinical experience. Cancer 116(9):2166–2173

    PubMed  Google Scholar 

  • Smeets SJ, Hesselink AT, Speel EJ, Haesevoets A, Snijders PJ, Pawlita M et al (2007) A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen. Int J Cancer 121(11):2465–2472

    Article  CAS  PubMed  Google Scholar 

  • Stenmark MH, McHugh JB, Schipper M, Walline HM, Komarck C, Feng FY et al (2014) Nonendemic HPV-positive nasopharyngeal carcinoma: association with poor prognosis. Int J Radiat Oncol Biol Phys 88(3):580–588

    Article  PubMed  PubMed Central  Google Scholar 

  • Thavaraj S, Stokes A, Guerra E, Bible J, Halligan E, Long A et al (2011) Evaluation of human papillomavirus testing for squamous cell carcinoma of the tonsil in clinical practice. J Clin Pathol 64(4):308–312

    Article  PubMed  Google Scholar 

  • Ukpo OC, Flanagan JJ, Ma XJ, Luo Y, Thorstad WL, Lewis JS Jr (2011) High-risk human papillomavirus E6/E7 mRNA detection by a novel in situ hybridization assay strongly correlates with p16 expression and patient outcomes in oropharyngeal squamous cell carcinoma. Am J Surg Pathol 35(9):1343–1350

    Article  PubMed  Google Scholar 

  • Weichert W, Schewe C, Denkert C, Morawietz L, Dietel M, Petersen I (2009) Molecular HPV typing as a diagnostic tool to discriminate primary from metastatic squamous cell carcinoma of the lung. Am J Surg Pathol 33(4):513–520

    Article  PubMed  Google Scholar 

  • Westra WH (2014) Detection of human papillomavirus (HPV) in clinical samples: evolving methods and strategies for the accurate determination of HPV status of head and neck carcinomas. Oral Oncol 50(9):771–779

    Article  PubMed  PubMed Central  Google Scholar 

  • Woo SB, Cashman EC, Lerman MA (2013) Human papillomavirus-associated oral intraepithelial neoplasia. Mod Pathol 26(10):1288–1297

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Oral Pathology, Centre for Oral Health Research, Newcastle University, Framlington Place, Newcastle upon Tyne, NE2 4BW, UK

    Max Robinson

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  1. Max Robinson
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Correspondence to Max Robinson .

Editor information

Editors and Affiliations

  1. Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Poznań, Poland

    Wojciech Golusiński

  2. Department of Otolaryngology-Head and Neck Surgery, VU University Medical Center/Cancer Center Amsterdam, Amsterdam, The Netherlands

    C. René Leemans

  3. Dept. of Head Medicine and Oral Health, University of Leipzig Clinic of Otolaryngology, Leipzig, Germany

    Andreas Dietz

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Robinson, M. (2017). HPV Testing of Head and Neck Cancer in Clinical Practice. In: Golusiński, W., Leemans, C., Dietz, A. (eds) HPV Infection in Head and Neck Cancer. Recent Results in Cancer Research, vol 206. Springer, Cham. https://doi.org/10.1007/978-3-319-43580-0_7

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  • DOI: https://doi.org/10.1007/978-3-319-43580-0_7

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-43578-7

  • Online ISBN: 978-3-319-43580-0

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