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Indigenous PAH-Degrading Bacteria in Oil-Polluted Marine Sediments from Patagonia: Diversity and Biotechnological Properties

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Biology and Biotechnology of Patagonian Microorganisms

Abstract

The diversity of indigenous bacteria recovered from polluted sediment samples from several coasts of Patagonia is described in this chapter. Selective enrichment cultures supplemented with naphthalene, phenanthrene, and pyrene allowed isolation of bacteria with the capability to degrade polycyclic aromatic hydrocarbons (PAHs). Bacterial communities of different composition (analyzed by denaturing gradient gel electrophoresis, DGGE) showed changes along with enrichment culture conditions. The ability of isolates to grow and remove different low and high molecular weight PAHs was demonstrated by detection of the residual substrate by HPLC. The presence and differential expression of naphthalene and catechol dioxygenase genes in several isolates suggest biodegradation potential in these sediments. Successful bacterial isolation with the ability to degrade PAH in pure and mixed cultures allows discussing the possibility to study and to further consider strategies to increase the intrinsic bioremediation opportunities on polluted coasts of Patagonia. Other biotechnological properties are also considered in this chapter, such as biosurfactant production and other biotransformations.

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Author information

Authors and Affiliations

  1. Centro de Investigación y Transferencia - Villa María (CIT-UNVM) CONICET, Arturo Jauretche 1555, Villa María, 5900, Córdoba, Argentina

    Paula Isaac

  2. Planta Piloto de Procesos Industriales Microbiológicos (PROIMI-CONICET), Av. Belgrano y Pje. Caseros, San Miguel de Tucumán, T4001MVB, Tucumán, Argentina

    Natalia Bourguignon, Daniela Maizel & Marcela A. Ferrero

  3. Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Ayacucho 471, San Miguel de Tucumán, T4001MVB, Tucumán, Argentina

    Marcela A. Ferrero

Authors
  1. Paula Isaac
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  2. Natalia Bourguignon
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  3. Daniela Maizel
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  4. Marcela A. Ferrero
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Corresponding author

Correspondence to Marcela A. Ferrero .

Editor information

Editors and Affiliations

  1. Patagonian Institute for the Study of Continental Ecosystems (IPEEC-CENPAT), National Scientific and Technical Research Council (CONICET), Puerto Madryn, Argentina

    Nelda Lila Olivera

  2. Andean-Patagonian Institute for Biological and Geoenvironmental Technologies (IPATEC) CONICET, National University of Comahue, Bariloche, Argentina

    Diego Libkind

  3. Industrial Fermentation Research and Development Center (CINDEFI), CONICET-UNLP, School of Science National University of La Plata, La Plata, Argentina

    Edgardo Donati

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Isaac, P., Bourguignon, N., Maizel, D., Ferrero, M.A. (2016). Indigenous PAH-Degrading Bacteria in Oil-Polluted Marine Sediments from Patagonia: Diversity and Biotechnological Properties. In: Olivera, N., Libkind, D., Donati, E. (eds) Biology and Biotechnology of Patagonian Microorganisms. Springer, Cham. https://doi.org/10.1007/978-3-319-42801-7_3

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