Skip to main content
SpringerLink
Log in

MMP-14 Triggered Fluorescence Contrast Agent

  • Conference paper
  • First Online:
Oxygen Transport to Tissue XXXVIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 923))

Abstract

Matrix metalloproteinase-14 (MMP-14) is involved in cancer invasion, metastasis, and angiogenesis. Therefore, it is considered to be a biomarker for aggressive cancer types, including some of the triple-negative breast cancer. Accurate (i.e., specific) and sensitive detection of MMP-14 can, thus, be important for the early diagnosis of and accurate prognosis for aggressive cancer, including the breast cancer caused by cell line MDA-MB 231. Fluorophore-mediated molecular sensing has been used for detecting biomarkers, for a long time. One way to increase the specificity of the sensing is designing the fluorophore to emit its fluorescence only when it encounters the biomarker of interest. When a fluorophore is placed on the surface of, or very close to a gold nanoparticle (GNP), its fluorescence is quenched. Applying this relationship between the GNP and fluorophore, we have developed a GNP-based, near-infrared fluorescent contrast agent that is highly specific for MMP-14. This agent normally emits only 14–17 % fluorescence of the free fluorophore. When the agent encounters MMP-14, its fluorescence gets fully restored, allowing MMP-14 specific optical signal emission.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Yao G, He P, Chen L, Hu X et al (2013) MT1-MMP in breast cancer: induction of VEGF-C correlates with metastasis and poor prognosis. Cancer Cell Int. doi:10.1186/1475-2867-13-98

    Google Scholar 

  2. Lu SY, Wang Y, Huang H et al (2013) Quantitative FRET imaging to visualize the invasiveness of live breast cancer cells. Plos One. doi:10.1371/journal.pone.0058569

    Google Scholar 

  3. Haage A, Nam DH, Ge X et al (2014) Matrix metalloproteinase-14 is a mechanically regulated activator of secreted MMPs and invasion. Biochem Biophys Res Commun 450:213–218

    Article  CAS  PubMed  Google Scholar 

  4. Curran S, Murray GI (1999) Matrix metalloproteinases in tumour invasion and metastasis. J Pathol 189:300–308

    Article  CAS  PubMed  Google Scholar 

  5. Malemud CJ (2006) Matrix metalloproteinases (MMPs) in health and disease: an overview. Front Biosci 11:1696–1701

    Article  CAS  PubMed  Google Scholar 

  6. Stamenkovic I (2000) Matrix metalloproteinases in tumor invasion and metastasis. Semin Cancer Biol 10:415–433

    Article  CAS  PubMed  Google Scholar 

  7. Kang KA, Wang JT (2014) Conditionally activating optical contrast agent with enhanced sensitivity via gold nanoparticle plasmon energy transfer: feasibility study. J Nanobiotechnol. doi:10.1186/s12951-014-0056-2

    Google Scholar 

  8. Kang KA, Wang JT, Jasinski JB et al (2011) Fluorescence manipulation by gold nanoparticles: from complete quenching to extensive enhancement. J Nanobiotechnol 9:16–29

    Article  CAS  Google Scholar 

  9. Wang JT, Achilefu S et al (2011) Gold nanoparticle-fluorophore complex for conditionally fluorescing signal mediator. Anal Chim Acta 695:96–104

    Article  CAS  PubMed  Google Scholar 

  10. Ye YP, Bloch S, Kao J et al (2005) Multivalent carbocyanine molecular probes: synthesis and applications. Bioconjug Chem 16:51–61

    Article  CAS  PubMed  Google Scholar 

  11. Wang JT, Moore J, Laulhe S et al (2012) Fluorophore-gold nanoparticle complex for sensitive optical biosensing and imaging. Nanotechnology. doi:10.1088/0957-4484/23/9/095501

    PubMed Central  Google Scholar 

  12. Kang KA, Wang JT (2014) Smart dual-mode fluorescent gold nanoparticle agents. Wiley Interdisciplinary Rev Nanomed Nanobiotechnol 6:398–409

    Article  CAS  Google Scholar 

  13. Kridel SJ, Sawai H, Ratnikov BI et al (2002) A unique substrate binding mode discriminates membrane type-1 matrix metalloproteinase from other matrix metalloproteinases. J Biol Chem 277:23788–23793

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial supported from the NIH Grant 1R15CA173693.

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Louisville, Louisville, KY, 40292, USA

    Mai-Dung Nguyen & Kyung A. Kang

Authors
  1. Mai-Dung Nguyen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kyung A. Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kyung A. Kang .

Editor information

Editors and Affiliations

  1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Qingming Luo

  2. Molecular Imaging Laboratory, Department of Radiology, Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Lin Z. Li

  3. Microvascular Measurements, St. Lorenzen, Italy

    David K. Harrison

  4. Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Hua Shi

  5. ISOTT Historian, Ellicott City, Maryland, USA

    Duane F. Bruley

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this paper

Cite this paper

Nguyen, MD., Kang, K.A. (2016). MMP-14 Triggered Fluorescence Contrast Agent. In: Luo, Q., Li, L., Harrison, D., Shi, H., Bruley, D. (eds) Oxygen Transport to Tissue XXXVIII. Advances in Experimental Medicine and Biology, vol 923. Springer, Cham. https://doi.org/10.1007/978-3-319-38810-6_54

Download citation

Publish with us

Policies and ethics

Search

Navigation