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Protective Effect of Dl-3-n-Butylphthalide on Recovery from Cardiac Arrest and Resuscitation in Rats

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Oxygen Transport to Tissue XXXVIII

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 923))

Abstract

In this study we investigated the effect of Dl-3-n-butylphthalide (NBP), a clinically used drug for stroke patients in China, on the recovery following cardiac arrest and resuscitation in rats. Male Wistar rats (3-month old) underwent cardiac arrest (12 min) and resuscitation. Rats were randomly assigned to the following groups: sham non-arrested group, vehicle group (vehicle-treated, 7 days before cardiac arrest and 4 days post-resuscitation), NBP pre-treated group (NBP-treated, 7 days before cardiac arrest), and NBP post-treated group (NBP-treated, 4 days post-resuscitation). Overall survival rates and hippocampal neuronal counts were determined in each group at 4 days post-resuscitation. Results showed that NBP pre-treated group (80 %) and NBP post-treated group (86 %) had significantly higher survival rates compared to that of the vehicle group (50 %). At 4 days of recovery, only about 20 % of hippocampal neurons were preserved in the vehicle group compared to the sham non-arrested group. The hippocampal CA1 cell counts in the NBP pre-treated group and NBP post-treated group were significantly higher than the counts in the vehicle group, about 50–60 % of the counts of non-arrested rats. The data suggest that NBP has both preventive and therapeutic effect on improving outcome following cardiac arrest and resuscitation, and NBP might be a potential early phase treatment for patients recovered from cardiac arrest and resuscitation.

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Acknowledgments

This study was supported by NIH grant NINDS 1 R01 NS46074. NBP was a gift from Shijiazhuang Pharma Grp NBP Co., LTD, Shijiazhuang, China.

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Authors and Affiliations

  1. Institute of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China

    Le Zhang

  2. Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA

    Michelle A. Puchowicz

  3. Department of Physiology and Biophysics, Case Western Reserve University, Robbins Building E732, 10900 Euclid Ave., Cleveland, OH, 44139, USA

    Joseph C. LaManna & Kui Xu

Authors
  1. Le Zhang
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  2. Michelle A. Puchowicz
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  3. Joseph C. LaManna
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  4. Kui Xu
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Corresponding author

Correspondence to Kui Xu .

Editor information

Editors and Affiliations

  1. Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Qingming Luo

  2. Molecular Imaging Laboratory, Department of Radiology, Britton Chance Laboratory of Redox Imaging, Johnson Research Foundation, Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Lin Z. Li

  3. Microvascular Measurements, St. Lorenzen, Italy

    David K. Harrison

  4. Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China

    Hua Shi

  5. ISOTT Historian, Ellicott City, Maryland, USA

    Duane F. Bruley

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© 2016 Springer International Publishing Switzerland

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Zhang, L., Puchowicz, M.A., LaManna, J.C., Xu, K. (2016). Protective Effect of Dl-3-n-Butylphthalide on Recovery from Cardiac Arrest and Resuscitation in Rats. In: Luo, Q., Li, L., Harrison, D., Shi, H., Bruley, D. (eds) Oxygen Transport to Tissue XXXVIII. Advances in Experimental Medicine and Biology, vol 923. Springer, Cham. https://doi.org/10.1007/978-3-319-38810-6_4

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