Abstract
The respiratory control system is not fully developed in newborn, and data suggest that adequate nutrition is important for the development of the respiratory control system. Infants need to be fed every 2–4 h to maintain appropriate energy levels, but a skip of feeding can occur due to social economical reasons or mild sickness of infants. Here, we asked questions if a short-term fasting (1) alters carotid body (CB) chemoreceptor activity and integrated function of the respiratory control system; (2) causes epigenetic modification within the respiratory control system. Mouse pups (<P14) were fasted for 3–6 h. Breathing became irregular and slow. The number and duration of apnea increased. Ventilatory response to hypoxia was also depressed even after the pups were returned to own dams. These effects were more prominent when the pups were younger and fasting time was longer. The hypoxic response of the carotid sinus nerve activity appeared to be depressed after fasting. Moreover, fasting increased global 5mC and 5-hmC content in DNA isolated from the CB but not DNA in the superior cervical ganglion (SCG). Methylation specific PCR (MSPCR) revealed that fasting increased methylation of leptin and socs3 genes. The results suggest fasting inhibits CB activity leading to hypoventilation, and low glucose does not compensate the low CB activity. Epigenetic effect on CB function/activity may be related to the prolonged effect of fasting on ventilation.
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References
Alvarez-Buylla A-B (1988) Carotid sinus receptors participate in glucose homeostasis. Respir Physiol 72:347–360
Balbir A, Lande B, Fitzgerald RS, Polotsky V, Mitzner W, Shirahata M (2008) Behavioral and respiratory characteristics during sleep in neonatal DBA/2J and A/J mice. Brain Res 1241:84–91
Bin-Jaliah I, Maskell PD, Kumar P (2004) Indirect sensing of insulin-induced hypoglycaemia by the carotid body in the rat. J Physiol 556:255–266
Dao T, Cheng RY, Revelo MP, Mitzner W, Tang W (2014) Hydroxymethylation as a novel environmental biosensor. Curr Environ Health Rep 1:1–10
de Brito Alves JL, Nogueira VO, de Oliveira GB, da Silva GS, Wanderley AG, Leandro CG, Costa-Silva JH (2014) Short- and long-term effects of a maternal low-protein diet on ventilation, O2/CO2 chemoreception and arterial blood pressure in male rat offspring. Br J Nutr 111:606–615
Edwards BA, Sands SA, Berger PJ (2013) Postnatal maturation of breathing stability and loop gain: the role of carotid chemoreceptor development. Respir Physiol Neurobiol 185:144–155
Gallego-Martin T, Fernandez-Martinez S, Rigual R, Obeso A, Gonzalez C (2012) Effects of low glucose on carotid body chemoreceptor cell activity studied in cultures of intact organs and in dissociated cells. Am J Physiol Cell Physiol 302:C1128–C1140
Gauda EB, Shirahata M, Mason A, Pichard LE, Kostuk EW, Chavez-Valdez R (2013) Inflammation in the carotid body during development and its contribution to apnea of prematurity. Respir Physiol Neurobiol 185:120–131
Gaultier C, Trang H, Dauger S, Gallego J (2005) Pediatric disorders with autonomic dysfunction: what role for PHOX2B? Pediatr Res 58:1–6
Hoe FM (2008) Hypoglycemia in infants and children. Adv Pediatr 55:367–384
Kinney HC, Richerson GB, Dymecki SM, Darnall RA, Nattie EE (2009) The brainstem and serotonin in the sudden infant death syndrome. Annu Rev Pathol 4:517–550
Koyama Y, Coker RH, Stone EE, Lacy DB, Jabbour K, Williams PE, Wasserman DH (2000) Evidence that carotid bodies play an important role in glucoregulation in vivo. Diabetes 49:1434–1442
Pardal R, López-Barneo J (2002) Low glucose-sensing cells in the carotid body. Nat Neurosci 5:197–198
Penatti EM, Barina AE, Raju S, Li A, Kinney HC, Commons KG, Nattie EE (2011) Maternal dietary tryptophan deficiency alters cardiorespiratory control in rat pups. J Appl Physiol 110:318–328
Spencer N, Logan S (2004) Sudden unexpected death in infancy and socioeconomic status: a systematic review. J Epidemiol Community Health 58:366–373
Tang W-Y, Ho S-M (2007) Epigenetic reprogramming and imprinting in origins of disease. Rev Endocr Metab Disord 8:173–182
Zhang M, Buttigieg J, Nurse CA (2007) Neurotransmitter mechanisms mediating low-glucose signalling in cocultures and fresh tissue slices of rat carotid body. J Physiol 578:735–750
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This work was funded, in part, by HL081345 and ES016817.
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Shirahata, M., Tang, WY., Kostuk, E.W. (2015). A Short-Term Fasting in Neonates Induces Breathing Instability and Epigenetic Modification in the Carotid Body. In: Peers, C., Kumar, P., Wyatt, C., Gauda, E., Nurse, C., Prabhakar, N. (eds) Arterial Chemoreceptors in Physiology and Pathophysiology. Advances in Experimental Medicine and Biology, vol 860. Springer, Cham. https://doi.org/10.1007/978-3-319-18440-1_20
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DOI: https://doi.org/10.1007/978-3-319-18440-1_20
Publisher Name: Springer, Cham
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