Abstract
Lack of tyrosine O Sulfation compromises both rod and cone electroretinographic responses emphasizing the importance of this post-translational modification for vision. To identify tyrosine sulfated proteins in retina, cow retinal lysates were subjected to immunoaffinity purification using an anti-sulfotyrosine antibody. The tyrosine sulfated proteins were eluted from the column using a sulfotyrosine pentapeptide and identified using mass spectrometry. Similarly, tyrosine sulfated proteins secreted by the 661W cell line were identified. Proteins identified were vitronectin, fibronectin, fibulin 2, nidogen, collagen V alpha 2, complement component 3 and C4 and fibrinogen beta. All proteins were subjected to analysis by ‘Sulfinator’ to determine potential sulfated tyrosines.
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References
Costagliola S, Panneels V, Bonomi M et al (2002) Tyrosine sulfation is required for agonist recognition by glycoprotein hormone receptors. EMBO J 21: 504–513
Fessler LI, Brosh S, Chapin S et al (1986) Tyrosine sulfation in precursors of collagen V. J Biol Chem 261:5034–5040
Hoffhines AJ, Damoc E, Bridges KG et al (2006) Detection and purification of tyrosine-sulfated proteins using a novel anti-sulfotyrosine monoclonal antibody. J Biol Chem 281:37877–37887
Hoffhines AJ, Jen CH, Leary JA et al (2009) Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem 284:3096–3105
Hortin G, Sims H, Strauss AW (1986) Identification of the site of sulfation of the fourth component of human complement. J Biol Chem 261:1786–1793
Kanan Y, Hoffhines A, Rauhauser A et al (2009) Protein tyrosine-O-sulfation in the retina. Exp Eye Res 89:559–567
Kanan Y, Hamilton RA, Moore KL et al (2012) Protein tyrosine-O-sulfation in bovine ocular tissues. Adv Exp Med Biol 723:835–841
Kanan Y, Brobst D, Han Z et al (2014a) Fibulin 2, a tyrosine O-sulfated protein, is up-regulated following retinal detachment. J Biol Chem 289:13419–13433
Kanan, Y, Siefert JC, Kinter M (2014b) Complement factor H, Vitronectin and Opticin are tyrosine sulfated proteins of the retinal pigmented epithelium. PLoS One e105409
Liu MC, Suiko M (1987) Tyrosine sulfation site is located in the C-terminal fibrin-binding domain in secreted fibronectin from rat embryo fibroblasts, line 3Y1. Arch Biochem Biophys 255:162–167
Monigatti F, Gasteiger E, Bairoch A, Jung E (2002) The Sulfinator: predicting tyrosine sulfation sites in protein sequences. Bioinformatics 18:769–70
Moore KL (2003) The biology and enzymology of protein tyrosine O-sulfation. J Biol Chem 278:24243–24246
Paulsson M, Dziadek M, Suchanek C et al (1985) Nature of sulphated macromolecules in mouse Reichert’s membrane. Evidence for tyrosine O-sulphate in basement-membrane proteins. Biochem J 231:571–579
Ramachandran V, Nollert MU, Qiu H et al (1999) Tyrosine replacement in P-selectin glycoprotein ligand-1 affects distinct kinetic and mechanical properties of bonds with P- and L-selectin. Proc Natl Acad Sci U S A 96:13771–13776
Sherry DM, Murray AR, Kanan Y et al (2010) Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy. Eur J Neurosci 32:1461–1472
Sherry DM, Kanan Y, Hamilton R et al (2012) Differential developmental deficits in retinal function in the absence of either protein tyrosine sulfotransferase-1 or -2. PLoS ONE 7:e39702
Tan E, Ding XQ, Saadi A et al (2004) Expression of cone-photoreceptor-specific antigens in a cell line derived from retinal tumors in transgenic mice. Investig Ophthalmol Vis Sci 45:764–768
Zhu JZ, Millard CJ, Ludeman JP et al (2011) Tyrosine sulfation influences the chemokine binding selectivity of peptides derived from chemokine receptor CCR3. Biochemistry 50:1524–1534
Acknowledgments
This study was supported by grants from Oklahoma Center for Advancement of Science and Technology (OCAST to YK), Foundation Fighting Blindness (MRA), the National Eye Institute EY018137 (MRA) and a P30EY021725. The funders had no role in design, collection and analysis of data, decision to publish, or manuscript preparation.
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Kanan, Y., Al-Ubaidi, M. (2016). Identification of Tyrosine O Sulfated Proteins in Cow Retina and the 661W Cell Line. In: Bowes Rickman, C., LaVail, M., Anderson, R., Grimm, C., Hollyfield, J., Ash, J. (eds) Retinal Degenerative Diseases. Advances in Experimental Medicine and Biology, vol 854. Springer, Cham. https://doi.org/10.1007/978-3-319-17121-0_86
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DOI: https://doi.org/10.1007/978-3-319-17121-0_86
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