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Carbohydrate Secondary and Tertiary Structure Using Raman Spectroscopy

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Polysaccharides

Abstract

Raman spectroscopy is a long-established analytical technique that has now proliferated into a variety of research tools that are able to identify and characterize almost any type of molecule under most conditions. As such, Raman spectroscopies are well suited to the study of carbohydrates, from simple monosaccharides to the largest glycosaminoglycans and from industrial bioreactors to in situ measurements on living cells. This review covers a range of examples of how Raman techniques are addressing the questions of glycobiologists working on diverse aspects of this fascinating but poorly understood class of biomolecules. Focus is placed on the application of Raman, surface-enhanced Raman, Raman optical activity, and related spectroscopies to characterizing carbohydrates of all types, with only a general introduction to the theory of the techniques themselves. Particular attention is also paid to the computational tools now regularly used by spectroscopists to analyze complex data. Although this review is aimed at the glycobiology community, the examples discussed also demonstrate to the expert spectroscopist how their techniques can impact on the exciting opportunities presented by working with carbohydrates.

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Authors and Affiliations

  1. Manchester Institute of Biotechnology and Faculty of Life Sciences, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK

    Shaun Thomas Mutter

  2. School of Applied Sciences, RMIT University, GPO Box 2476, Melbourne, 3001, VIC, Australia

    Ewan William Blanch

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  1. Shaun Thomas Mutter
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Correspondence to Ewan William Blanch .

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  1. Botany Department, M.L.Sukhadia University, Udaipur, India

    Kishan Gopal Ramawat

  2. Groupe d’Etude des Substances Végétales à Activité Biologique, Université de Bordeaux, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France

    Jean-Michel Mérillon

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Mutter, S.T., Blanch, E.W. (2015). Carbohydrate Secondary and Tertiary Structure Using Raman Spectroscopy. In: Ramawat, K., Mérillon, JM. (eds) Polysaccharides. Springer, Cham. https://doi.org/10.1007/978-3-319-16298-0_36

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