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Increased N-Acetyltaurine in Serum and Urine After Endurance Exercise in Human

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Taurine 9

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 803))

Abstract

N-acetyltaurine (NAT) that is a novel metabolite of taurine conjugated with acetate has been reported to increase in urine during alcohol catabolism. Because acetate production would be also increased by endurance exercise, NAT might be produced during the exercise. This study aimed to determine the NAT in serum and urine after endurance exercises. Serum and urinary levels of taurine and NAT after a transient exercise or full-marathon race were measured by HPLC-MS/MS system. In addition, the synthesis of NAT was evaluated in cultured skeletal muscle cell (C2C12 myotube) exposed to taurine and acetate. Serum taurine and NAT concentrations were significantly increased immediately after full-marathon compared to those before the marathon, and recovered to the normal levels after a day. Urinary excretions of taurine and NAT were markedly higher in 24-h period after the transient exercise compared to those before the exercise. In culture cell, the excretion of NAT to medium was significantly increased following the exposing to both taurine and acetate. The present study could confirm the significant increase of NAT in serum and urine after endurance exercise, and it is suggesting that taurine may conjugate with excess acetyl-group in the skeletal muscles during exercise.

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Abbreviations

NAT:

N-acetyltaurine

ACS2:

Acetyl-CoA synthase 2

GM:

Growth medium

DM:

Differentiation medium

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Acknowledgements

This work was supported by Kakenhi grants Number (25750334).

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Authors and Affiliations

  1. Tokyo Medical University Ibaraki Medical Center, Ami, Ibaraki, Japan

    Teruo Miyazaki, Akira Honda, Yasunori Miyamoto & Yasushi Matsuzaki

  2. University of Tsukuba, Tsukuba, Ibaraki, Japan

    Keisuke Ishikura, Song-Gyu Ra, Shoichi Komine & Hajime Ohmori

Authors
  1. Teruo Miyazaki
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  2. Keisuke Ishikura
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  3. Akira Honda
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  4. Song-Gyu Ra
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  5. Shoichi Komine
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  6. Yasunori Miyamoto
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  7. Hajime Ohmori
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  8. Yasushi Matsuzaki
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Corresponding author

Correspondence to Teruo Miyazaki .

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Editors and Affiliations

  1. Chair of Immunology, Medical College Jagiellonian University, Krakow, Poland

    Janusz Marcinkiewicz

  2. Department of Pharmacology, College of Medicine University of South Alabama, Mobile, Alabama, USA

    Stephen W. Schaffer

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Miyazaki, T. et al. (2015). Increased N-Acetyltaurine in Serum and Urine After Endurance Exercise in Human. In: Marcinkiewicz, J., Schaffer, S. (eds) Taurine 9. Advances in Experimental Medicine and Biology, vol 803. Springer, Cham. https://doi.org/10.1007/978-3-319-15126-7_5

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