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Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioprocess Development

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Animal Cell Culture

Part of the book series: Cell Engineering ((CEEN,volume 9))

Abstract

The genetic sequencing of Chinese hamster ovary cells has initiated a systems biology era for biotechnology applications. In addition to genomics, critical ‘omics data sets also include proteomics, transcriptomics and metabolomics. Recently, the use of proteomics in cell lines for recombinant protein production has increased significantly because proteomics can track changes in protein levels for different cell lines over time, which can be advantageous for bioprocess development and optimization. Specifically, the identification of proteins that affect cell culture processes can aid efforts in media development and cell line engineering to improve growth or productivity, delay the onset of apoptosis, or utilize nutrients efficiently. Mass-spectrometry based and other proteomics methods can provide for the detection of thousands of proteins from cell culture and bioinformatics analysis serves to identify and quantify protein levels. Optimizations of sample preparations and database development, including a detailed CHO proteome now available, have improved the quantity and accuracy of identified proteins. The applications are widespread and expanding, thus suggesting numerous applications of proteomics and combined ‘omics experiments in coming years.

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Authors and Affiliations

  1. Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA

    Kelley Heffner, Amit Kumar, Deniz Baycin-Hizal & Michael Betenbaugh

  2. Network Engineering of Eukaryotic Cell Factories, Technical University of Denmark, Kgs Lyngby, Denmark

    Christian Schroeder Kaas

  3. Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA

    Amit Kumar

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  1. Kelley Heffner
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  2. Christian Schroeder Kaas
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  3. Amit Kumar
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  4. Deniz Baycin-Hizal
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  5. Michael Betenbaugh
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Correspondence to Michael Betenbaugh .

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Editors and Affiliations

  1. University College Dublin, Dublin, Ireland

    Mohamed Al-Rubeai

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Heffner, K., Kaas, C.S., Kumar, A., Baycin-Hizal, D., Betenbaugh, M. (2015). Proteomics in Cell Culture: From Genomics to Combined ‘Omics for Cell Line Engineering and Bioprocess Development. In: Al-Rubeai, M. (eds) Animal Cell Culture. Cell Engineering, vol 9. Springer, Cham. https://doi.org/10.1007/978-3-319-10320-4_19

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