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Neuronal differentiation and long-term culture of the human neuroblastoma line SH-SY5Y

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Neuropsychiatric Disorders An Integrative Approach

Part of the book series: Journal of Neural Transmission. Supplementa ((NEURALTRANS,volume 72))

Abstract

Parkinson’s disease (PD) is the second most prevalent neurodegenerative disorder in industrialized countries. Present cell culture models for PD rely on either primary cells or immortal cell lines, neither of which allow for long-term experiments on a constant population, a crucial requisite for a realistic model of slowly progressing neurodegenerative diseases.

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References

  • Barzilai A, Melamed E, Shirvan A (2001) Is there a rationale for neuro-protection against dopamine toxicity in Parkinson’s disease? Cell Mol Neurobio 121: 215–235

    Article  Google Scholar 

  • Biedler JL, Helson L, Spengler BA (1973) Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res 33: 2643–2652

    PubMed  CAS  Google Scholar 

  • Biedler JL, Roffler-Tarlov S, Schachner M, Freedman LS (1978) Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res 38: 3751–3757

    PubMed  CAS  Google Scholar 

  • Binder LI, Frankfurter A, Rebhun LI (1985) The distribution of tau in the mammalian central nervous system. J Cell Biol 101: 1371–1378

    Article  PubMed  CAS  Google Scholar 

  • Blander G, de Oliveira RM, Conboy CM, Haigis M, Guarente L (2003) Superoxide dismutase 1 knock-down induces senescence in human fibroblasts. J Biol Chem 278: 38966–38969

    Article  PubMed  CAS  Google Scholar 

  • Bove J, Prou D, Perier C, Przedborski S (2005) Toxin-induced models of Parkinson’s disease. NeuroRx 2: 484–494

    Article  PubMed  Google Scholar 

  • Cajal SR y (1928) Degeneration and regeneration of the nervous system. University Press, London

    Google Scholar 

  • Dauer W, Przedborski S (2003) Parkinson’s disease: mechanisms and models. Neuron 39: 889–909

    Article  PubMed  CAS  Google Scholar 

  • Duggal N, Hammond RR (2002) Nestin expression in ganglioglioma. Exp Neurol 174: 89–95

    Article  PubMed  CAS  Google Scholar 

  • Edsjo A, Lavenius E, Nilsson H, Hoehner JC, Simonsson P, Culp LA, Martinsson T, Larsson C, Pahlman S (2003) Expression of trkB in human neuroblastoma in relation to MYCN expression and retinoic acid treatment. Lab Invest 83: 813–823

    PubMed  Google Scholar 

  • Encinas M, Iglesias M, Liu Y, Wang H, Muhaisen A, Cena V, Gallego C, Cornelia JX (2000) Sequential treatment of SH-SY5Y cells with retinoic acid and brain-derived neurotrophic factor gives rise to fully differentiated, neurotrophic factor-dependent, human neuron-like cells. J Neurochem 75: 991–1003

    Article  PubMed  CAS  Google Scholar 

  • Farooqui SM (1994) Induction of adenylate cyclase sensitive dopamine D2-receptors in retinoic acid induced differentiated human neuro-blastoma SH-SY5Y cells. Life Sci 55: 1887–1893

    Article  PubMed  CAS  Google Scholar 

  • Gaardsvoll H, Obendorf D, Winkler H, Bock E (1988) Demonstration of immunochemical identity between the synaptic vesicle proteins synaptin and synaptophysin/p38. FEBS Lett 242: 117–120

    Article  PubMed  CAS  Google Scholar 

  • Gage FH (2002) Neurogenesis in the adult brain. J Neurosci 22: 612–613

    PubMed  CAS  Google Scholar 

  • Gates MA, Torres EM, White A, Fricker-Gates RA, Dunnett SB (2006) Re-examining the ontogeny of substantia nigra dopamine neurons. Eur J Neurosci 23: 1384–1390

    Article  PubMed  Google Scholar 

  • Gille G, Rausch WD, Hung ST, Moldzio R, Ngyuen A, Janetzky B, Engfer A, Reichmann H (2002) Protection of dopaminergic neurons in primary culture by lisuride. J Neural Transm 109: 157–169

    Article  PubMed  CAS  Google Scholar 

  • Hashemi SH, Li JY, Ahlman H, Dahlstrom A (2003) SSR2(a) receptor expression and adrenergic/cholinergic characteristics in differentiated SH-SY5Y cells. Neurochem Res 28: 449–460

    Article  PubMed  CAS  Google Scholar 

  • Herman GE (2002) Mouse models of human disease: lessons learned and promises to come. ILAR J 43: 55–56

    PubMed  CAS  Google Scholar 

  • Laifenfeld D, Klein E, Ben Shachar D (2002) Norepinephrine alters the expression of genes involved in neuronal sprouting and differentiation: relevance for major depression and antidepressant mechanisms. J Neurochem 83: 1054–1064

    Article  PubMed  CAS  Google Scholar 

  • Lee JE, Hollenberg SM, Snider L, Turner DL, Lipnick N, Weintraub H (1995) Conversion of xenopus ectoderm into neurons by neurod, a basic helix-loop-helix protein. Science 268: 836–844

    Article  PubMed  CAS  Google Scholar 

  • Lee MK, Tuttle JB, Rebhun LI, Cleveland DW, Frankfurter A (1990) The expression and posttranslational modification of a neuron-specific beta-tubulin isotype during chick embryogenesis. Cell Motil Cytoskeleton 17: 118–132

    Article  PubMed  CAS  Google Scholar 

  • Ma Q, Kintner C, Anderson DJ (1996) Identification of neurogenin, a vertebrate neuronal determination gene. Cell 87: 43–52

    Article  PubMed  CAS  Google Scholar 

  • Maden M, Hind M (2003) Retinoic acid, a regeneration-inducing molecule. Dev Dyn 226: 237–244

    Article  PubMed  CAS  Google Scholar 

  • Maruyama W, Benedetti MS, Takahashi T, Naoi M (1997) A neurotoxin N-methyl(R)salsolinol induces apoptotic cell death in differentiated human dopaminergic neuroblastoma SH-SY5Y cells. Neurosci Lett 232: 147–150

    Article  PubMed  CAS  Google Scholar 

  • Melino G, Thiele CJ, Knight RA, Piacentini M (1997) Retinoids and the control of growth/death decisions in human neuroblastoma cell lines. J Neurooncol 31: 65–83

    Article  PubMed  CAS  Google Scholar 

  • Minuth WW, Schumacher K, Strehl R, Kloth S (2000) Physiological and cell biological aspects of perfusion culture technique employed to generate differentiated tissues for long term biomaterial testing and tissue engineering. J Biomater Sci Polym Ed 11: 495–522

    Article  PubMed  CAS  Google Scholar 

  • Minuth WW, Steiner P, Strehl R, Schumacher K, de Vries U, Kloth S (1999) Modulation of cell differentiation in perfusion culture. Exp Nephrol 7: 394–406

    Article  PubMed  CAS  Google Scholar 

  • Mullen RJ, Buck CR, Smith AM (1992) NeuN, a neuronal specific nuclear protein in vertebrates. Development 116: 201–211

    PubMed  CAS  Google Scholar 

  • Nestler EJ, Aghajanian GK (1997) Molecular and cellular basis of addiction. Science 278: 58–63

    Article  PubMed  CAS  Google Scholar 

  • Nicolini G, Miloso M, Zoia C, Di Silvestro A, Cavaletti G, Tredici G (1998) Retinoic acid differentiated SH-SY5Y human neuroblastoma cells: an in vitro model to assess drug neurotoxicity. Anticancer Res 18: 2477–2481

    PubMed  CAS  Google Scholar 

  • Pahlman S, Hoehner JC, Nanberg E, Hedborg F, Fagerstrom S, Gestblom C, Johansson I, Larsson U, Lavenius E, Ortoft E, Soderholm H (1995) Differentiation and Survival Influences of Growth-Factors in Human Neuroblastoma. Eur J Cancer 31A: 453–458

    Article  PubMed  CAS  Google Scholar 

  • Pleasure SJ, Page C, Lee VM (1992) Pure, postmitotic, polarized human neurons derived from NTera 2 cells provide a system for expressing exogenous proteins in terminally differentiated neurons. J Neurosci 12: 1802–1815

    PubMed  CAS  Google Scholar 

  • Presgraves SP, Ahmed T, Borwege S, Joyce JN (2004) Terminally differentiated SH-SY5Y cells provide a model system for studying neuro-protective effects of dopamine agonists. Neurotox Res 5: 579–598

    Article  PubMed  Google Scholar 

  • Rakic P (2002) Adult neurogenesis in mammals: an identity crisis. J Neurosci 22: 614–618

    PubMed  Google Scholar 

  • Rasband WS (2006) Image J. U. S. National Institutes of Health, Bethesda, Maryland, USA, http://rsb.info.nih.gov/ij/

  • Rebhan M, Vacun G, Bayreuther K, Rosner H (1994) Altered ganglioside expression by SH-SY5Y cells upon retinoic acid-induced neuronal differentiation. Neuroreport 5: 941–944

    Article  PubMed  CAS  Google Scholar 

  • Ross RA, Spengler BA, Biedler JL (1983) Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J Natl Cancer Inst 71: 741–747

    PubMed  CAS  Google Scholar 

  • Sherer TB, Trimmer PA, Borland K, Parks JK, Bennett JP Jr, Tuttle JB (2001) Chronic reduction in complex I function alters calcium signaling in SH-SY5Y neuroblastoma cells. Brain Res 891: 94–105

    Article  PubMed  CAS  Google Scholar 

  • Singh US, Pan J, Kao YL, Joshi S, Young KL, Baker KM (2003) Tissue transglutaminase mediates activation of RhoA and MAP kinase pathways during retinoic acid-induced neuronal differentiation of SH-SY5Y cells. J Biol Chem 278: 391–399

    Article  PubMed  CAS  Google Scholar 

  • Storch A, Ludolph AC, Schwarz J (2004) Dopamine transporter: involvement in selective dopaminergic neurotoxicity and degeneration. J Neural Transm 111: 1267–1286

    Article  PubMed  CAS  Google Scholar 

  • Timpl R, Brown JC (1994) The laminins. Matrix Biol 14: 275–281

    Article  PubMed  CAS  Google Scholar 

  • Willets JM, Lambert DG, Lunec J, Griffiths HR (1995) Studies on the neurotoxicity of 6,7-dihydroxy-l-methyl-l,2,3,4-tetrahydroiso-quinoline(salsolinol) in SH-SY5Y cells. Eur J Pharmacol 293: 319–326

    Article  PubMed  CAS  Google Scholar 

  • Wood JG, Mirra SS, Pollock NJ, Binder LI (1986) Neurofibrillary tangles of Alzheimer disease share antigenic determinants with the axonal microtubule-associated protein tau (tau). Proc Natl Acad Sci USA 83: 4040–4043

    Article  PubMed  CAS  Google Scholar 

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Constantinescu, R., Constantinescu, A.T., Reichmann, H., Janetzky, B. (2007). Neuronal differentiation and long-term culture of the human neuroblastoma line SH-SY5Y. In: Gerlach, M., Deckert, J., Double, K., Koutsilieri, E. (eds) Neuropsychiatric Disorders An Integrative Approach. Journal of Neural Transmission. Supplementa, vol 72. Springer, Vienna. https://doi.org/10.1007/978-3-211-73574-9_3

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  • DOI: https://doi.org/10.1007/978-3-211-73574-9_3

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-73573-2

  • Online ISBN: 978-3-211-73574-9

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