Abstract
The study of DNA from ancient material adds molecular data from fossils to evolutionary biological research. Examples of successful retrieval and characterization of Pleistocene DNA include mammoths [1-8], mastodon [4], ground sloths [9-11], a sabre-tooth tiger [12], a cave bear [13], the Neanderthal type specimen [14], and modern human remains [15]. Although the fossils studied were from various locations, a few conditions are generally shared. Most samples are derived from permafrost, i. e., the mammoth samples, or cold caves, i. e., an extinct mylodontid ground sloth. Based on these studies and the fact that the only reproducible results date to the late Pleistocene, success is predicted to be confined to samples no older than 50,000 years of age
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hagelberg E, Thomas MG, Cook CEJ, Sher AV et al. (1994) DNA from ancient mammoth bones. Nature 370: 333–334
Höss M, Pääbo S and Vereshchagin NK (1994) Mammoth DNA sequences. Nature 370: 333
Hauf J, Baur A, Chalwatzis N, Zimmermann FK et al. (1995) Selective amplification of a mammoth mitochondrial cytochrome b fragment using an elephant specific primer. Curr. Genet. 27: 486–487
Yang, H., Golenberg EM and Shoshani J (1996) Phylogenetic resolution within the Elephantidae using fossil DNA sequences from the American mastodon (Mammut americanum). Proc. Natl. Acad. Sci. USA 93: 1190–1194
Ozawa T, Hayashi S, Mikhelson VM (1997) Phylogenetic position of the mammoth and Steller’s sea cow within tethytheria demonstrated by mitochondrial DNA sequences. J. Mol. Evol. 44: 406–413
Noro M, Masuda R, Dubrovo IA, Yoshida MC et al. (1998) Molecular phylogenetic inference of the woolly mammoth Mammuthus primigenius based on the complete sequences of the mitochondrial cytochrome b and 12S ribosomal RNA genes. J. Mol. Evol. 46: 314–326
Derenko M, Malyarchuk, Shields GF (1997) Mitochondrial cytochrome b sequence from a 33,000 year-old woolly mammoth (Mammuthus primigenius). Ancient Biomolecules 1: 149–153
Greenwood AD, Capelli C, Possnert G, Pääbo S (1999) Nuclear DNA sequences from late Pleistocene megafauna. Mol. Biol. Evol. 16(11): 1466–1473
Höss M, Dilling A, Currant A, Pääbo S (1996) Molecular phylogeny of the extinct ground sloth Mylodon darwinii. Proc. Natl. Acad. Sci. USA 93: 181–185
Taylor P (1996) Reproducibility of ancient DNA sequences from extinct Pleistocene fauna. Mol. Biol. Evol. 13: 2839
Poinar H, Hofreiter M, Spaulding WG, Martin PS, et al. (1998) Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis. Science 281: 402–406
Janczewski DN, Yuhki N, Gilbert DA, Jefferson GT et al. (1992) Molecular phylogenetic inference from saber-tooth cat fossils of Rancho La Brea. Proc. Natl. Acad. Sci. USA 89: 9769–9773
Hanni C, Laudet V, Stehelin D and Taberlet P (1994) Tracking the origins of the cave bear (Ursus spelaeus) by mitochondrial DNA sequencing. Proc. Natl. Acad. Sci. USA 91: 12336–12340
Krings M, Stone A, Schmitz RW, Krainitzki H, Stoneking M et al. (1997) Neanderthal DNA sequences and the origin of modern humans. Cell 90: 19–30
Büraud-Colomb E, Roubin R, Martin J, Maroc N et al. (1995) Human beta-globin gene polymorphisms characterized in DNA extracted from ancient bones 12,000 years old. Am. J. Hum. Genet. 57: 1267–1274
Lindahl T (1993) Instability and decay of the primary structure of DNA. Nature 362: 709–715
Höss M, Jaruga P, Zastawny TH, Dizdaroglu M et al. (1996) DNA damage and DNA sequence retrieval from ancient tissues. Nucleic Acids Res. 24: 13041307
Zischler H, Höss M, Handto, von Haesler A et al. (1995) Detecting dinosaur DNA. Science 268: 1192–1193
Boom R, Sol CJ, Salimans MM, Janson CL et al. (1990) Rapid and simple method for purification of necleic acids. J. Clin. Microbiol 28(3): 495–503
Höss M, Pääbo S (1993) DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids. Res. 21: 3913–3914
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: A laboratory manual. In: N Ford, C Nolan and M Ferguson (eds): Cold Spring Harbor Laboratory Press, New York
Handto, Richards M, Trommsdorff M, Kilger C et al. (1994) Molecular geneticanalysis of the Tyrolean ice man. Science 264: 1775–1778
Greenwood AD, Pääbo S (1999) Nuclear insertion sequences of mitochondrial DNA predominate in hair but not in blood of elephants. Mol. Ecol. 8: 133–137
Zhang D, Hewitt GM (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends Ecol. Evol. 11: 247–251.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Springer Basel AG
About this chapter
Cite this chapter
Greenwood, A.D. (2002). Late Pleistocene DNA Extraction and Analysis. In: DeSalle, R., Giribet, G., Wheeler, W. (eds) Techniques in Molecular Systematics and Evolution. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-8125-8_13
Download citation
DOI: https://doi.org/10.1007/978-3-0348-8125-8_13
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-7643-6257-7
Online ISBN: 978-3-0348-8125-8
eBook Packages: Springer Book Archive