Abstract
It has been known for many years that transmissible spongiform encephalopathy (TSE) agents exhibit strain variation [1], a phenomenon that has been studied most extensively in experimental mouse models. Numerous distinct TSE strains have been isolated in mice from a range of scrapie, bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease (CJD) sources. The methods used for TSE strain discrimination are based on simple observations of disease characteristics, the most useful being the length of the incubation period of the disease and the type and extent of the pathological changes seen hi the brains of infected animals [24]. Formal strain typing protocols in mice, based on incubation periods and neuropathology, have been used extensively as tools in basic research, for example in studies exploring the nature of TSE agents.
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Reference
Further reading
Dickinson AG, Meikle VMH (1971) Host-genotype and agent effects in scrapie incubation: change in allelic interaction with different strains of agent. Mol Gen Genet 112: 73–79
Fraser H, Dickinson AG (1968) The sequential development of the brain lesions of scrapie in three strains of mice. J Comp Pathol 78: 301–311
Bruce ME (2003) TSE strain variation. Br Med Bull 66: 99–108
Bruce ME, McConnell I, Fraser H et al (1991) The disease characteristics of different strains of scrapie in Sinc congenic mouse lines: implications for the nature of the agent and host control of pathogenesis. J Gen Virol 72: 595–603
Bruce ME, Boyle A, Cousens S et al (2002) Strain characterization of natural sheep scrapie and comparison with BSE. J Gen Virol 83: 695–704
Reference
Bruce ME (2003) TSE strain variation. Br Med Bull 66: 99–108
Dickinson AG, Meikle VMH (1971) Host- 8 genotype and agent effects in scrapie incubation: change in allelic interaction with different strains of agent. Mol Gen Genet 112: 73–79
Fraser H, Dickinson AG (1968) The se- 9 quential development of the brain le-sions of scrapie in three strains of mice. J Comp Pathol 78: 301–311
Bruce ME, McConnell I, Fraser H et al (1991) The disease characteristics of different strains of scrapie in Sinc con-genic mouse lines: implications for the nature of the agent and host control of 11 pathogenesis. J Gen Virol 72: 595–603
Westaway D, Goodman PA, Mirenda CA et al (1987) Distinct prion proteins in short and long scrapie incubation period 12 mice. Cell 51: 651–662
Dickinson AG, Meikle VMH, Fraser H (1968) Identification of a gene which controls the incubation period of some strains of scrapie agent in mice. J Comp Pathol 78: 293–299
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Moore RC, Hope J, McBride PA et al (1998) Mice with gene targetted prion protein alterations show that Prnp, Sinc and Prni are congruent. Nat Genet 18: 118–125
Fraser H (1993) Diversity in the neuropathology of scrapie-like diseases in animals. Br Med Bull 49: 792–809
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Bruce, M.E., Boyle, A., McConnell, I. (2004). TSE Strain Typing in Mice. In: Lehmann, S., Grassi, J. (eds) Techniques in Prion Research. Methods and Tools in Biosciences and Medicine. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-7949-1_10
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DOI: https://doi.org/10.1007/978-3-0348-7949-1_10
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-7643-2224-3
Online ISBN: 978-3-0348-7949-1
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