Abstract
Angiogenesis is a natural and vital phenomenon of neovascularization that occurs from pre-existing vasculature, being present in many physiological processes, namely in development, reproduction and regeneration. Being a highly dynamic and tightly regulated process, its abnormal expression can be on the basis of several pathologies. For that reason, angiogenesis has been a subject of major interest among the scientific community, being transverse to different areas and founding particular attention in tissue engineering and cancer research fields. Microfluidics has emerged as a powerful tool for modelling this phenomenon, thereby surpassing the limitations associated to conventional angiogenic models. Holding a tremendous flexibility in terms of experimental design towards a specific goal, microfluidic systems can offer an unlimited number of opportunities for investigating angiogenesis in many relevant scenarios, namely from its fundamental comprehension in normal physiological processes to the identification and testing of new therapeutic targets involved on pathological angiogenesis. Additionally, microvascular 3D in vitro models are now opening up new prospects in different fields, being used for investigating and establishing guidelines for the development of next generation of 3D functional vascularized grafts. The promising applications of this emerging technology in angiogenesis studies are herein overviewed, encompassing fundamental and applied research.
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Acknowledgments
The authors would like to acknowledge the financial support provided by the Portuguese Foundation for Science and Technology (FCT) through the project B-FABULUS (PTDC/BBB-ECT/2690/2014) and Fun4TE (PTDC/EMD-EMD/31367/2017). The FCT distinctions attributed to J. Silva-Correia (IF/00115/2015) and J. Miguel Oliveira (IF/01285/2015) under the Investigator FCT program are also greatly acknowledged.
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Costa, L., Reis, R.L., Silva-Correia, J., Oliveira, J.M. (2020). Microfluidics for Angiogenesis Research. In: Oliveira, J., Reis, R. (eds) Biomaterials- and Microfluidics-Based Tissue Engineered 3D Models. Advances in Experimental Medicine and Biology, vol 1230. Springer, Cham. https://doi.org/10.1007/978-3-030-36588-2_7
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