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Identification of Posttranslational Modifications (PTMs) of Proteins by Mass Spectrometry

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1140))

Abstract

There are only 30,000 human genes, which, according to the central dogma from biology, it means that there should be 30,000 mRNA and 30,000 proteins. However, there are at least 1-2 million protein entities that are expressed in a cell at a given time. This is primarily due to alternative splicing in different cells and tissues, which may lead to expression of different protein isoforms within one cell, but also different protein isoforms in different tissues. A new level of complexity of proteins and protein isoforms is then given by posttranslational modifications (PTMs) of proteins. Here, we discuss the PTMs in proteins and how they are identified by mass spectrometry and proteomics, with specific examples on identification of acetylation, phosphorylation, glycosylation, alkylation, hydroxinonenal-modification or assignment of intramolecular and intermolecular disulfide bridges.

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Acknowledgements

We would like to thank the past and current lab members for the friendly work environment.

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Authors and Affiliations

  1. Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Roshanak Aslebagh, Kelly L. Wormwood, Devika Channaveerappa, Armand G. Ngounou Wetie, Alisa G. Woods & Costel C. Darie

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  1. Roshanak Aslebagh
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  2. Kelly L. Wormwood
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  3. Devika Channaveerappa
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  4. Armand G. Ngounou Wetie
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  5. Alisa G. Woods
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  6. Costel C. Darie
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Correspondence to Roshanak Aslebagh or Costel C. Darie .

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  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Alisa G. Woods

  2. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Costel C. Darie

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Aslebagh, R., Wormwood, K.L., Channaveerappa, D., Wetie, A.G.N., Woods, A.G., Darie, C.C. (2019). Identification of Posttranslational Modifications (PTMs) of Proteins by Mass Spectrometry. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_11

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