Abstract
Antibodies are an integral part of biological and medical research. In addition, immunoglobulins are used in many diagnostic tests and are becoming increasingly important in the therapy of diseases. To express antibodies recombinantly, the immunoglobulin heavy and light chains are usually cloned into two different expression plasmids. Here, we describe a method for recombinant antibody expression from a single plasmid.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lindenmann J (1984) Origin of the terms ‘antibody’ and ‘antigen’. Scand J Immunol 19(4):281–285
Feldmann M, Maini RN (2001) Anti-TNF alpha therapy of rheumatoid arthritis: what have we learned? Annu Rev Immunol 19: 163–196
Plosker GL, Figgitt DP (2003) Rituximab: a review of its use in non-Hodgkin’s lymphoma and chronic lymphocytic leukaemia. Drugs 63(8):803–843
Medzhitov R, Janeway CA Jr (2002) Decoding the patterns of self and nonself by the innate immune system. Science 296(5566):298–300
Salinas GF, Braza F et al (2012) The role of B lymphocytes in the progression from autoimmunity to autoimmune disease. Clin Immunol 146(1):34–45
Market E, Papavasiliou FN (2003) V(D)J recombination and the evolution of the adaptive immune system. PLoS Biol 1(1):E16
Pelanda R, Torres RM (2006) Receptor editing for better or for worse. Curr Opin Immunol 18(2):184–190
Maul RW, Gearhart PJ (2010) Controlling somatic hypermutation in immunoglobulin variable and switch regions. Immunol Res 47(1–3):113–122
Tomita M, Tsumoto K (2011) Hybridoma technologies for antibody production. Immunotherapy 3(3):371–380
Kipriyanov SM, Little M (1999) Generation of recombinant antibodies. Mol Biotechnol 12(2): 173–201
Gritzmacher CA (1988) Producing novel antibodies by expression of recombinant immunoglobulin genes. Year Immunol 3:260–274
Humphreys DP, Glover DJ (2001) Therapeutic antibody production technologies: molecules, applications, expression and purification. Curr Opin Drug Discov Devel 4(2):172–185
Crosnier C, Staudt N et al (2010) A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. BMC Biol 8:76
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media, New York
About this protocol
Cite this protocol
Müller-Sienerth, N., Crosnier, C., Wright, G.J., Staudt, N. (2014). Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid. In: Ossipow, V., Fischer, N. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 1131. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-992-5_14
Download citation
DOI: https://doi.org/10.1007/978-1-62703-992-5_14
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-991-8
Online ISBN: 978-1-62703-992-5
eBook Packages: Springer Protocols