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Introduction to Cotranscriptional RNA Splicing

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Spliceosomal Pre-mRNA Splicing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1126))

Abstract

The discovery that many intron-containing genes can be cotranscriptionally spliced has led to an increased understanding of how splicing and transcription are intricately intertwined. Cotranscriptional splicing has been demonstrated in a number of different organisms and has been shown to play roles in coordinating both constitutive and alternative splicing. The nature of cotranscriptional splicing suggests that changes in transcription can dramatically affect splicing, and new evidence suggests that splicing can, in turn, influence transcription. In this chapter, we discuss the mechanisms and consequences of cotranscriptional splicing and introduce some of the tools used to measure this process.

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Acknowledgments

We would like to thank members of the Johnson Lab for critical reading of the manuscript and apologize to any colleagues whose work is not referenced due to unintentional oversight or space constraints. Funding was provided by the National Institutes of General Medical Sciences (GM085474), the National Science Foundation (MCB-1051921), and an IRACDA fellowship to E.C.M. (K12 GM068524).

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Authors and Affiliations

  1. Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA

    Evan C. Merkhofer & Peter Hu

  2. Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Terasaki Life Sciences Building Room 5159 610 Charles Young Dr. E, Los Angeles, CA, 90095-7239, USA

    Tracy L. Johnson

Authors
  1. Evan C. Merkhofer
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  2. Peter Hu
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  3. Tracy L. Johnson
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Editors and Affiliations

  1. University of California, Irvine, California, USA

    Klemens J. Hertel

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Merkhofer, E.C., Hu, P., Johnson, T.L. (2014). Introduction to Cotranscriptional RNA Splicing. In: Hertel, K. (eds) Spliceosomal Pre-mRNA Splicing. Methods in Molecular Biology, vol 1126. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-980-2_6

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  • DOI: https://doi.org/10.1007/978-1-62703-980-2_6

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  • Publisher Name: Humana Press, Totowa, NJ

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