Abstract
Uridine addition at the 3′ end of RNAs (i.e., uridylation) emerges as a critical posttranscriptional modification promoting RNA degradation. Uridylation has been notably linked to the degradation of small RNAs, correlated with the 5′ shortening of RISC-cleaved transcripts and the degradation of mRNAs. We describe here a method based on 3′ RACE (3′ Rapid Amplification of cDNA End) PCR that has been successfully used to investigate nucleotide addition at the 3′ end of RISC-cleaved transcripts and full-length mRNAs in plants.
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Acknowledgment
This work was supported by Centre National de la Recherche Scientifique (France).
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Sement, F.M., Gagliardi, D. (2014). Detection of Uridylated mRNAs. In: Rorbach, J., Bobrowicz, A. (eds) Polyadenylation. Methods in Molecular Biology, vol 1125. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-971-0_4
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DOI: https://doi.org/10.1007/978-1-62703-971-0_4
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Publisher Name: Humana Press, Totowa, NJ
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