Abstract
Ribonucleic acid (RNA) extraction is the necessary first step in many protocols, primarily to investigate genes and gene expression. RNA comes in a variety of forms: total RNA, ribosomal RNA, messenger RNA (mRNA), and small interfering RNA (siRNA) to name a few. In some instances, total RNA is all that is required; however most applications will require the enrichment for some particular form of RNA. In plants, including cereals, total RNA is a mixture of many types of RNA and enrichment is generally required. In this protocol, the TRIzol® method of RNA extraction from cereal leaf material is described, as it is a relatively simple technique.
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References
Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159
TRIzol® Reagent technical insert (Life Technologies Australia, Pty Ltd)
RNaseZap® technical insert (Life Technologies Australia, Pty Ltd)
RNAlater® technical insert (Life Technologies Australia, Pty Ltd)
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© 2014 Springer Science+Business Media New York
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Pattemore, J.A. (2014). RNA Extraction from Cereal Vegetative Tissue. In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_3
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DOI: https://doi.org/10.1007/978-1-62703-715-0_3
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-714-3
Online ISBN: 978-1-62703-715-0
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