Abstract
Embryonic stem (ES) cells are unique models for investigating early development and cell differentiation. First identified in mouse and later in other mammals, these cells have also been isolated in avian species. Here, using chicken as a model, we describe a set of protocols allowing the isolation, maintenance, genetic modification, differentiation, and injection of the chicken embryonic stem (cES) cells into embryos for obtaining chimeric animals.
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References
Chambers I, Smith A (2004) Self renewal of teratocarcinoma and embryonic stem cells. Oncogene 23:7150–7160
Pain B, Clark ME, Shen M et al (1996) Long term in vitro culture and characterisation of avian embryonic stem cells with multiple morphogenetic potentialities. Development 122:2339–2348
Petitte JN, Liu G, Yang Z (2004) Avian pluripotent stem cells. Mech Dev 121:1159–1168
Pain B, Chenevier P, Samarut J (1999) Chicken embryonic stem cells and transgenic strategies. Cells Tissues Organs 165:212–219
Acloque H, Risson V, Birot AM et al (2001) Identification of a new gene family specifically expressed in chicken embryonic stem cells and early embryo. Mech Dev 103:79–91
Pain B, Samarut J, Valarche I et al (2001) Exogenous protein expression system in avian system. Patent WO03043415
Lavial F, Acloque H, Bertocchini F et al (2007) The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells. Development 134:3549–3563
Acknowledgments
This work was supported by grants from ANR (ANR 09 BLAN 0141 01).
We thank the laboratory members for their help and comments.
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© 2013 Springer Science+Business Media, New York
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Aubel, P., Pain, B. (2013). Chicken Embryonic Stem Cells: Establishment and Characterization. In: Alberio, R. (eds) Epiblast Stem Cells. Methods in Molecular Biology, vol 1074. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-628-3_11
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DOI: https://doi.org/10.1007/978-1-62703-628-3_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-627-6
Online ISBN: 978-1-62703-628-3
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