Abstract
Metabolomics is a comprehensive profiling tool used to identify qualitatively and quantitatively all the metabolites present in a biological system. As the number of metabolites in a living being is assumed to be around 30,000, it is necessary to use an adequate extraction procedure and a good analytical technique to perform metabolomic analyses. Each analytical platform used in metabolomics has both advantages and disadvantages in terms of the sensitivity and resolution for metabolites to be detected. Of the methods, nuclear magnetic resonance (NMR) spectroscopy has been proved to have several advantages over other MS-based methods. NMR provides an efficient, robust, and nondestructive metabolomics analysis of crude extracts or samples as well as easy quantitation without calibration curves for each metabolite, although it shows relatively low sensitivity than MS-based methods. Thus, NMR-based metabolomics have been often used for the first step to capture the insight of the metabolome of organisms. This chapter presents general steps of NMR-based metabolomic analysis involved in the study of biodiversity.
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References
Verpoorte R, Choi YH, Kim HK (2007) NMR-based metabolomics at work in phytochemistry. Phytochem Rev 6:3–14
Schripsema J (2010) Application of NMR in plant metabolomics: techniques, problems and prospects. Phytochem Anal 21:14–21
Verpoorte R, Choi YH, Mustafa NR, Kim HK (2008) Metabolomics: back to basics. Phytochem Rev 7:525–537
Ebbels TMD, Cavill R (2009) Bioinformatic methods in NMR-based metabolic profiling. Prog Nucl Magn Reson Spectrosc 55:361–374
Verpoorte R, Choi YH, Kim HK (2010) Metabolomics: will it stay? Phytochem Anal 21:2–3
Kim HK, Choi YH, Verpoorte R (2010) NMR-based metabolomic analysis of plants. Nat Protoc 5:536–549
Kim HK, Choi YH, Verpoorte R (2011) NMR-based plant metabolomics: where do we stand, where do we go? Trends Biotechnol 29:267–275
Van der Kooy F, Maltese F, Choi YH, Kim HK, Verpoorte R (2009) Quality control of herbal material and phytopharmaceuticals with MS and NMR based metabolic fingerprinting. Planta Med 75:763–775
Choi YH, Sertic S, Kim HK, Wilson EG, Michopoulosa F, Lefeber AWM, Erkelens C, Verpoorte R (2005) Classification of Ilex species based on metabolomic fingerprinting using nuclear magnetic resonance and multivariate data analysis. J Agric Food Chem 53:1237–1245
Kim HK, Saifullah K, Wilson EG, Kricun SDP, Meissner A, Goraler S, Deelder AM, Choi YH, Verpoorte R (2010) Metabolic classification of South American Ilex species by NMR-based metabolomics. Phytochemistry 71:773–784
Hendrawati O, Yao Q, Choi YH, Kim HK, Linthorst HJM, Erkelens C, Lefeber AWM, Verpoorte R (2006) Metabolic differentiation of Arabidopsis treated with methyl jasmonate using nuclear magnetic resonance spectroscopy. Plant Sci 170:1118–1124
Jahangir M, Kim HK, Choi YH, Verpoorte R (2008) Metabolomic response of Brassica rapa submitted to pre-harvest bacterial contamination. Food Chem 107:362–368
Leiss KA, Choi YH, Abdel-Farid IB, Verpoorte R, Klinkhamer PGL (2009) NMR metabolomics of thrips (Frankliniella occidentalis) resistance in Senecio hybrids. J Chem Ecol 35:219–229
Ali K, Maltese F, Figueiredo A, Rex M, Fortes AM, Zyprian E, Pais MS, Verpoorte R, Choi YH (2012) Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points. Plant Sci 191–192:100–107
Choi YH, Kim HK, Hazekamp A, Erkelens C, Lefeber AW, Verpoorte R (2004) Metabolomic differentiation of Cannabis sativa cultivars using 1H NMR spectroscopy and principal component analysis. J Nat Prod 67:953–957
Kim HK, Choi YH, Erkelens C, Lefeber AWM, Verpoorte R (2005) Metabolic fingerprinting of Ephedra species using 1H-NMR spectroscopy and principal component analysis. Chem Pharm Bull 53:105–109
Frédérich M et al (2004) Metabolomic analysis of Strychnos nux-vomica, icaja and ignatii extracts by 1H nuclear magnetic resonance spectrometry and multivariate analysis techniques. Phytochemistry 65:1993–2001
Yang SY, Kim HK, Lefeber AWM, Erkelens C, Angelova C, Choi YH, Verpoorte R (2006) Application of two dimensional nuclear magnetic resonance spectroscopy to quality control of ginseng commercial products. Planta Med 72:364–369
Kim HK, Verpoorte R (2010) Sample preparation for plant metabolomics. Phytochem Anal 21:4–13
Acknowledgments
This work was partly supported by National Institute of Horticultural and Herbal Science, Rural Development Administration of Korea (Project No: PJ008700).
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Pimenta, L.P.S., Kim, H.K., Verpoorte, R., Choi, Y.H. (2013). NMR-Based Metabolomics: A Probe to Utilize Biodiversity. In: Roessner, U., Dias, D. (eds) Metabolomics Tools for Natural Product Discovery. Methods in Molecular Biology, vol 1055. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-577-4_9
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DOI: https://doi.org/10.1007/978-1-62703-577-4_9
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