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Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System

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Plant Transposable Elements

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1057))

Abstract

Transposable genetic elements are considered to be ubiquitous. Despite this, their mutagenic capacity has been exploited in only a few species. The main plant species are maize, Antirrhinum, and Petunia. Representatives of all three major groups of class II elements, viz., hAT-, CACTA- and Mutator-like elements, have been identified in Petunia. Here we focus on the research “history” of the Petunia two-element Act1–dTph1 system and the development of its application in forward- and reverse-genetics studies.

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Acknowledgments

It is with great pleasure and thankfulness that I acknowledge the contribution of so many to the development of the Petunia transposon system described here; in chronological order: Marcel Beld, Eli Vrijlandt, Eric Souer, Wim Veerman, Henk Huits, Ronald Koes, Jan Zethof, Tamara Maes, Dirk van den Broeck, Peter de Keukeleire, and Michiel Vandenbussche, to mention those most heavily involved. Funding for parts of this research was provided by the Netherlands Organization for Scientific Research (H.H. and M.V.), the Flemish IWT (T.M., D.vd B. P.K.), the EC Biotech Program Bio4-CT97-2217 (M.V.), and a HORIZON grant (050-71-036) (M.V.) from the Netherlands Genomics Initiative; the development of the “454-TD” approach was performed in cooperation with Keygene (Wageningen).

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Authors and Affiliations

  1. Department of Plant Genetics/IWWR/Radboud University, Nijmegen, The Netherlands

    Tom Gerats

  2. Plant Genetics, IWWR, Radboud University Nijmegen, Nijmegen, The Netherlands

    Jan Zethof

  3. UMR 5667 CNRS-INRA-ENS Lyon-Unversité Lyon I, RDP Laboratory, ENS Lyon, Lyon, Cedex, France

    Michiel Vandenbussche

Authors
  1. Tom Gerats
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  2. Jan Zethof
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  3. Michiel Vandenbussche
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Editor information

Editors and Affiliations

  1. Dept. Genetics, Development & Cell Biology, Iowa State University, Ames, Iowa, USA

    Thomas Peterson

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Gerats, T., Zethof, J., Vandenbussche, M. (2013). Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System. In: Peterson, T. (eds) Plant Transposable Elements. Methods in Molecular Biology, vol 1057. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-568-2_16

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  • DOI: https://doi.org/10.1007/978-1-62703-568-2_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-567-5

  • Online ISBN: 978-1-62703-568-2

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