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Antisense Effects of PNAs in Bacteria

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Peptide Nucleic Acids

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1050))

Abstract

Antisense peptide nucleic acid (PNA) can be used to control bacterial gene expression. PNAs are designed to target sequences within messenger RNA and knock-down gene expression. PNAs targeted to the translation initiation region of mRNA are particularly effective and result in mRNA degradation and reduced protein expression from the targeted gene. The antisense effects can be sufficient to alter phenotypes and even kill bacteria. PNAs provide useful tools for the study of bacterial gene function, and with improvements in cell uptake antisense PNAs may find applications as antimicrobial agents. This chapter details methods for designing antisense PNAs and testing their activities in bacteria, including suggestions for control experiments.

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Author information

Authors and Affiliations

  1. Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, London, UK

    Shan Goh & Liam Good

  2. Department of Biology, Newcastly University, Newcastle, UK

    Jem Stach

Authors
  1. Shan Goh
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  2. Jem Stach
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  3. Liam Good
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Editor information

Editors and Affiliations

  1. Dept. Cellular & Molecular Medicine, University of Copenhagen Faculty of Health Sciences, Copenhagen N, Denmark

    Peter E Nielsen

  2. NIH NIDDK, Bethesda, USA

    Daniel H. Appella

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Goh, S., Stach, J., Good, L. (2014). Antisense Effects of PNAs in Bacteria. In: Nielsen, P., Appella, D. (eds) Peptide Nucleic Acids. Methods in Molecular Biology, vol 1050. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-553-8_18

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  • DOI: https://doi.org/10.1007/978-1-62703-553-8_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-552-1

  • Online ISBN: 978-1-62703-553-8

  • eBook Packages: Springer Protocols

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