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Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1051))

Abstract

Novel heterofunctional glyoxyl-agarose supports were prepared. These supports contained the maximal concentration of glyoxyl groups to promote maximization of covalent immobilization and groups’ capability to adsorb proteins by various mechanisms (e.g., ionic exchange, metal-chelate formation). Immobilization on various supports makes it possible to orientate and rigidify an enzyme in various regions of its surface. The use of different heterofunctional supports allowed for obtaining catalysts with different activity, stability, and selectivity properties.

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Author information

Authors and Affiliations

  1. Institute of Catalysis, CSIC, CAMPUS UAM-Cantoblanco, Madrid, Spain

    Cesar Mateo, Javier Rocha-Martin & Juan M. Bolivar

  2. Instituto de Investigación en Ciencias de la Alimentación (CIAL) CSIC-UAM, Madrid, Spain

    Gloria Fernandez-Lorente

  3. Instituto de Catalisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

Authors
  1. Cesar Mateo
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  2. Gloria Fernandez-Lorente
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  3. Javier Rocha-Martin
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  4. Juan M. Bolivar
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  5. Jose M. Guisan
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Editor information

Editors and Affiliations

  1. Instituto de Catlisis y Petroleoquimica, CSIC, Madrid, Spain

    Jose M. Guisan

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Mateo, C., Fernandez-Lorente, G., Rocha-Martin, J., Bolivar, J.M., Guisan, J.M. (2013). Oriented Covalent Immobilization of Enzymes on Heterofunctional-Glyoxyl Supports. In: Guisan, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 1051. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-550-7_6

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  • DOI: https://doi.org/10.1007/978-1-62703-550-7_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-549-1

  • Online ISBN: 978-1-62703-550-7

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