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Peptide Release, Side-Chain Deprotection, Work-Up, and Isolation

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Peptide Synthesis and Applications

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1047))

Abstract

After having successfully synthesized a peptide, it has to be released from the solid support, unless it is being used for on-resin display. The linker and, in some cases, the cleavage mixture determine the C-terminal functionality of the released peptide. In most cases, the peptide is released with concomitant removal of side-chain protecting groups. However, some combinations of linkers and side-chain protecting groups enable a two-stage procedure, either using orthogonal chemistry or graduated labilities. Herein, we describe protocols for the release of peptides from the most commonly used linker types providing a variety of different C-terminal functionalities, including acids, amides, amines, and aldehydes. Moreover, suggestions for determination of peptide purity and for storage conditions are provided.

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Author information

Authors and Affiliations

  1. IGM, Faculty of Life Sciences, University of Copenhagen, Gubra, Hørsholm, Denmark

    Søren L. Pedersen

  2. Department of Chemistry, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark

    Knud J. Jensen

Authors
  1. Søren L. Pedersen
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  2. Knud J. Jensen
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Editor information

Editors and Affiliations

  1. IGM, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark

    Knud J. Jensen

  2. IGM, Faculty of Science, University of Copenhagen, Glostrup, Denmark

    Pernille Tofteng Shelton

  3. IGM, Faculty of Life Sciences, University of Copenhagen, Hørsholm, Denmark

    Søren L. Pedersen

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Pedersen, S.L., Jensen, K.J. (2013). Peptide Release, Side-Chain Deprotection, Work-Up, and Isolation. In: Jensen, K., Tofteng Shelton, P., Pedersen, S. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology, vol 1047. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-544-6_3

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  • DOI: https://doi.org/10.1007/978-1-62703-544-6_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-543-9

  • Online ISBN: 978-1-62703-544-6

  • eBook Packages: Springer Protocols

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