Abstract
The topology of integral membrane proteins with a weak topological tendency can be influenced when fused to tags, such as these used for topological determination or protein purification. Here, we describe a technique for topology determination of an untagged membrane protein. This technique, optimized for bacterial cells, allows the visualization of the protein in the native environment and incorporates the substituted-cysteine accessibility method.
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Acknowledgments
SS is Mathilda Marks-Kennedy Professor of Biochemistry at the Hebrew University of Jerusalem. Work in our laboratory is supported by National Institutes of Health Grant NS16708 and Grant 11/08 from the Israel Science Foundation.
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Nasie, I., Steiner-Mordoch, S., Schuldiner, S. (2013). Topology Determination of Untagged Membrane Proteins. In: Rapaport, D., Herrmann, J. (eds) Membrane Biogenesis. Methods in Molecular Biology, vol 1033. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-487-6_8
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DOI: https://doi.org/10.1007/978-1-62703-487-6_8
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