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Production and Characterization of Humanized Rag2−/−γc−/− Mice

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Mouse Models of Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1031))

Abstract

Mice reconstituted with human immune cells represent a model to study the development and functionality of the human immune system. Recent improvements in humanized mice have resulted in multi-lineage hematopoiesis, prolonged human cell engraftment that is detectable in many mouse organs, and the ability to generate de novo human innate and adaptive immune responses. Here, we describe the methods used to produce and characterize humanized Rag2−/−γc−/− mice.

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Acknowledgments

This work was supported by a Mentoring Environment Grant from Brigham Young University.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, USA

    Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner & Bradford K. Berges

Authors
  1. Freddy M. Sanchez
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  2. German I. Cuadra
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  3. Stanton J. Nielsen
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  4. Anne Tanner
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  5. Bradford K. Berges
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Editor information

Editors and Affiliations

  1. , Dept. of Biomedical Sciences & Pathobiol, Virginia Tech, Duckpond Drive, Blacksburg, 24061, Virginia, USA

    Irving C. Allen

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Sanchez, F.M., Cuadra, G.I., Nielsen, S.J., Tanner, A., Berges, B.K. (2013). Production and Characterization of Humanized Rag2−/−γc−/− Mice. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1031. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-481-4_2

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  • DOI: https://doi.org/10.1007/978-1-62703-481-4_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-480-7

  • Online ISBN: 978-1-62703-481-4

  • eBook Packages: Springer Protocols

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