Abstract
Glycosyltransferases control the biosynthesis of glycans expressed in cells. Alterations in glycosylation in the gastrointestinal tract stem from deregulation of glycosyltransferase expression. These modifications can be detected in situ by cell and tissue immunolabelling techniques which are highly informative in physiological and pathological contexts. The protocols described here are single and double-labelling immunofluorescence techniques that allow the detection of a specific glycosyltransferase and, in the case of double labelling, the concomitant detection of the glycosyltransferase and its glycan product.
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Acknowledgments
This work was supported by Fundação para a Ciência e a Tecnologia—FCT: project PIC/IC/82716/2007 to CAR and grant SFRH/BD/40563/2007 to JG.
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Gomes, J., Reis, C.A. (2013). Immunodetection of Glycosyltransferases in Gastrointestinal Tissues. In: Brockhausen, I. (eds) Glycosyltransferases. Methods in Molecular Biology, vol 1022. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-465-4_26
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DOI: https://doi.org/10.1007/978-1-62703-465-4_26
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