Abstract
Laser-scanning in vivo confocal microscopy (IVCM) of the cornea is becoming an increasingly popular tool to examine the living human cornea with cellular-level detail in both healthy and pathologic states. Here, we describe the use of the IVCM technique to examine the processes of tissue healing and regeneration in the living human eye after biomaterial implantation. The regenerative response can be assessed by performing longitudinal IVCM imaging of a laboratory-made, cell-free biomaterial, after direct implantation into a pathologic eye as a primary alternative to human donor tissue transplantation.
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References
Patel DV, McGhee CNJ (2007) Contemporary in vivo confocal microscopy of the living human cornea using white light and laser scanning techniques: a major review. Clin Experiment Ophthalmol 35:71–88
Guthoff RF, Zhivov A, Stachs O (2009) In vivo confocal microscopy, an inner vision of the cornea – a major review. Clin Experiment Ophthalmol 37:100–117
Niederer RL, McGhee CNJ (2010) Clinical in vivo confocal microscopy of the human cornea in health and disease. Prog Retin Eye Res 29:30–58
Reichard M, Hovakimyan M, Wree A, Meyer-Lindenberg A, Nolte I, Junghans C et al (2010) Comparative in vivo confocal microscopical study of the cornea anatomy of different laboratory animals. Curr Eye Res 35:1072–1080
Guthoff RF, Baudouin C, Stave J (2006) Atlas of confocal laser scanning in-vivo microscopy in ophthalmology. Springer, Heidelberg
Stachs O, Zhivov A, Kraak R, Stave J, Guthoff R (2007) In vivo three-dimensional confocal laser scanning microscopy of the epithelial nerve structure in the human cornea. Graefe’s Arch Clin Exp Ophthalmol 245:569–575
Acknowledgments
The authors wish to acknowledge the contribution of Kimberley Merrett (University of Ottawa Eye Institute) and Yuwen Liu (CooperVision), for developing and fabricating, respectively, the biomaterial samples for ex vivo imaging; and the Canadian Stem Cell Network and NSERC Canada for grant funding to May Griffith for biomaterials development. The authors also thank Professor Joachim Stave, University of Rostock, for discussions concerning the Z-Scan Motor Drive for the Rostock Cornea Module.
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Lagali, N., Griffith, M., Fagerholm, P. (2013). In Vivo Confocal Microscopy of the Cornea to Assess Tissue Regenerative Response After Biomaterial Implantation in Humans. In: Wright, B., Connon, C. (eds) Corneal Regenerative Medicine. Methods in Molecular Biology, vol 1014. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-432-6_15
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DOI: https://doi.org/10.1007/978-1-62703-432-6_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-431-9
Online ISBN: 978-1-62703-432-6
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