Abstract
Human adipose-derived adult stem cells (ASCs) represent a unique population of multipotent stem cells. Their utility in a variety of tissue engineering applications, and as a model system for the study of molecular mechanisms of differentiation, is well established. In addition, their relative abundance, ease of isolation from human subcutaneous lipoaspirates, and functional stability make them an excellent physiologically relevant platform. Here, we describe detailed procedures for handling and purification of ASCs from lipoaspirate, as well as their expansion, cryopreservation, quality control, and functional assays.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Guilak F, Awad HA, Fermor B et al (2004) Adipose-derived adult stem cells for cartilage tissue engineering. Biorheology 41:389–399
Guilak F, Lott KE, Awad HA et al (2006) Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol 206:229–237
Gimble J, Guilak F (2003) Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy 5:362–369
Gimble JM, Grayson W, Guilak F et al (2011) Adipose tissue as a stem cell source for musculoskeletal regeneration. Front Biosci (Schol Ed) 3:69–81
De Toni F, Poglio S, Youcef AB et al (2011) Human adipose-derived stromal cells efficiently support hematopoiesis in vitro and in vivo: a key step for therapeutic studies. Stem Cells Dev 20:2127–2138
Kilroy GE, Foster SJ, Wu X et al (2007) Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol 212:702–709
Lea-Currie YR, Duffin DJ, Buehrer BM (2011) Use of adipose-derived stem cells in high-throughput screening to identify modulators of lipogenesis. Methods Mol Biol 702:359–368
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Buehrer, B.M., Cheatham, B. (2013). Isolation and Characterization of Human Adipose-Derived Stem Cells for Use in Tissue Engineering. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_1
Download citation
DOI: https://doi.org/10.1007/978-1-62703-363-3_1
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-362-6
Online ISBN: 978-1-62703-363-3
eBook Packages: Springer Protocols