Abstract
Metabolomics provides an invaluable means to interrogate the function of “orphan” enzymes, i.e., those whose endogenous substrates are not known. Here we describe a high-performance liquid chromatography-coupled mass spectrometry (HPLC-MS)-based metabolomics approach to identify an endogenous substrate of an orphan cytochrome P450.
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References
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Cheng, Q., Guengerich, F.P. (2013). Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_6
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DOI: https://doi.org/10.1007/978-1-62703-321-3_6
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-320-6
Online ISBN: 978-1-62703-321-3
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