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Tissue Microarrays: Construction and Use

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Pancreatic Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 980))

Abstract

Tissue microarrays (TMAs) enable high-throughput tissue analysis by selecting a large number of ­paraffin-embedded donor tissue block cores and transferring these tissue cores into a positionally encoded array in the recipient TMA block. Once TMAs are constructed, a variety of analysis may be performed on the arrays including histochemical, immunohistochemical, or immunofluorescent staining, and in situ hybridization for DNA or RNA. TMAs offer a cost-effective method for performing parallel analysis of a large number of tissue samples. In this chapter we outline the method of TMA construction with an emphasis on providing useful information in the analysis of a variety of pancreatic neoplasms, including pancreatic adenocarcinomas and pre-invasive lesions. The technique of TMA construction in this chapter is restricted to the use of formalin-fixed paraffin-embedded tissue.

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Acknowledgments

The author would like to acknowledge the assistance of Lin Yang, Rina Wu, Ting Ting Wu, and Mariam Amrani, MD, in the preparation of this chapter.

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Authors and Affiliations

  1. Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA

    Helen Remotti

  2. The New York Presbyterian Hospital, New York, NY, USA

    Helen Remotti

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  1. Helen Remotti
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Correspondence to Helen Remotti .

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Editors and Affiliations

  1. , Irving Cancer Research Center, Columbia University, St. Nicholas Avenue 1130, New York, 10032, New York, USA

    Gloria H. Su

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Remotti, H. (2013). Tissue Microarrays: Construction and Use. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_2

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  • DOI: https://doi.org/10.1007/978-1-62703-287-2_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-286-5

  • Online ISBN: 978-1-62703-287-2

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