Abstract
The nematode Caenorhabditis elegans provides a versatile and expedient platform for the genetic and molecular dissection of mechanisms underlying senescent decline and aging. Indeed, pioneering studies in this organism revealed the first genes and pathways directly influencing lifespan. In this chapter, we present routine, mainstream methods, developed for monitoring aging and senescent decline in C. elegans. These procedures allow the assessment of lifespan parameters on solid growth media that are typically used for rearing nematodes.
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Acknowledgement
Work in the authors’ laboratory is funded by grants from the European Research Council (ERC), the European Commission Framework Programmes, and the Greek Ministry of Education.
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Lionaki, E., Tavernarakis, N. (2013). Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis. In: Galluzzi, L., Vitale, I., Kepp, O., Kroemer, G. (eds) Cell Senescence. Methods in Molecular Biology, vol 965. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-239-1_31
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DOI: https://doi.org/10.1007/978-1-62703-239-1_31
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Publisher Name: Humana Press, Totowa, NJ
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