Abstract
Ovules are the major female reproductive organs in higher plants. In addition, ovules of Arabidopsis thaliana are successfully used as model system to study plant organogenesis. Here we describe two microscopic techniques to analyze Arabidopsis ovule development from the organ to the cellular level in a rapid and reproducible fashion. Both methods are of great value when comparing the morphology of wild-type and mutant ovule development.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Chevalier D, Sieber P, Schneitz K (2002) The genetic and molecular control of ovule development. In: O’ Neill SD, Roberts JA (eds) Plant reproduction. Sheffield Academic Press, Sheffield
Colombo L, Battaglia R, Kater MM (2008) Arabidopsis ovule development and its evolutionary conservation. Trends Plant Sci 13:444–450
Kelley DR, Gasser CS (2009) Ovule development: genetic trends and evolutionary considerations. Sex Plant Reprod 22:229–234
Shi D, Yang W (2011) Ovule development in Arabidopsis: progress and challenge. Curr Opin Plant Biol 14:74–80
Schneitz K, Hülskamp M, Pruitt RE (1995) Wild-type ovule development in Arabidopsis thaliana: a light microscope study of cleared whole-mount tissue. Plant J 7:731–749
Schneitz K et al (1997) Dissection of sexual organ ontogenesis: a genetic analysis of ovule development in Arabidopsis thaliana. Development 124:1367–1376
Running MP, Clark SE, Meyerowitz EM (1995) Confocal microscopy of the shoot apex. Methods Cell Biol 49:217–229
Smyth DR, Bowman JL, Meyerowitz EM (1990) Early flower development in Arabidopsis. Plant Cell 2:755–767
Kurup S et al (2005) Marking cell lineages in living tissues. Plant J 42:444–453
Acknowledgments
Our work is currently supported by grants SCHN 723/1-3, SCHN 723/3-2, and SCHN 723/6-1 from the German Research Council (DFG) to KS.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Enugutti, B., Oelschner, M., Schneitz, K. (2013). Microscopic Analysis of Ovule Development in Arabidopsis thaliana . In: De Smet, I. (eds) Plant Organogenesis. Methods in Molecular Biology, vol 959. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-221-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-62703-221-6_7
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-220-9
Online ISBN: 978-1-62703-221-6
eBook Packages: Springer Protocols