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Studying Ubiquitination of MHC Class I Molecules

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

Abstract

The covalent attachment of ubiquitin to a protein is one of the most common post-translational modifications and regulates diverse eukaryotic cellular processes. Ubiquitination of MHC class I was first described in the context of viral proteins which target MHC class I for degradation in the endoplasmic reticulum and at the cell surface. Study of viral-induced MHC class I degradation has been extremely instructive in elucidating cellular pathways for degradation of membrane and secretory proteins. More recently, ubiquitination of endogenous MHC class I heavy chains which fail to achieve their native conformation and undergo endoplasmic-reticulum associated degradation has been demonstrated.

In this chapter we describe methods for identification of endogenous ubiquitinated MHC class I heavy chains by MHC class I-immunoprecipitation and ubiquitin-specific immunoblot or by metabolic labeling and immunoprecipitation.

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Acknowledgements

This work was supported by the Wellcome Trust and the NIHR Cambridge Biomedical Research Centre.

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Authors and Affiliations

  1. Cambridge Institute for Medical Research, University of Cambridge, Addenbrooke’s Hospital, Cambridge, UK

    Marian L. Burr & Jessica M. Boname

  2. Cambridge Institute for Medical Research, Addenbrooke’s Hospital, University of Cambridge, Cambridge, UK

    Paul J. Lehner

Authors
  1. Marian L. Burr
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  2. Jessica M. Boname
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  3. Paul J. Lehner
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Corresponding author

Correspondence to Paul J. Lehner .

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Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Burr, M.L., Boname, J.M., Lehner, P.J. (2013). Studying Ubiquitination of MHC Class I Molecules. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_9

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

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